FF - Trabalhos de Conclusão de Curso

URI Permanente para esta coleção

http://200.137.215.59//handle/ri/1418

Navegar

Agora exibindo 1 - 14 de 14

Administração bucal de biomoléculas terapêuticas: descrição dos desafios e das estratégias tecnológicas descritas na literatura
(Universidade Federal de Goiás, 2024-01-22) Pimenta, Anna Karolyna Nery; Dall’Ara, Inghrid Ferreira; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Ferreira, Renata Luiza; Pires, Jader
Buccal delivery of therapeutic biomolecules is an attractive route of administration that has been extensively studied in recent years. This route bypasses first-pass hepatic and intestinal metabolism and can be used for drugs both locally and systemically, in addition to having a milder pH, when compared to the stomach, and has few proteolytic enzymes. However, although it is a route of administration that has numerous advantages, there are obstacles such as the limited absorption area and barrier properties that the mucosa presents. To overcome these obstacles, strategies were planned with promising approaches, such as the use of nanoparticles in oral films, gels and tablets, as well as the use of vaccines using microneedles that guarantee an improvement in the bioavailability of biopharmaceuticals/antigens administered via the oral route. Overall, the aim of this review was discussing recent advances on nanotechnological approaches developed to date to improve buccal delivery of therapeutic biomolecules.
Atividade leishmanicida de três derivados de chalcona e sua associação com a rigidez da membrana avaliada pela espectroscopia de RPE
(Universidade Federal de Goiás, 2023-08-11) Borges, Ellyêssa do Nascimento; Alonso, Antonio; Alonso, Antonio; Menegatti, Ricardo; Silva, Kleber Santiago Freitas e
Leishmaniasis is a neglected tropical disease that affects the world's poorest populations. Currently, therapeutic options have high toxicity, with prolonged therapeutic regimens. Still, the emergence of resistance highlights the need to seek new therapeutic agents. Chalcones are precursors of flavonoid biosynthesis with clinical potential for the treatment of leishmaniasis. Novel chalcone-like compounds, LQFM332 (4), LQFM333 (5), and LQFM341 (6), were synthesized and their activity against Leishmania (L.) amazonensis was evaluated using electron paramagnetic resonance (EPR) spectroscopy and assays of cell viability. Compound 4 was synthesized from the molecular hybridization of vanillin (9) and butylated hydroxytoluene (BHT), forming a chalcone scaffold. BHT was replaced by another apocynin to give compound 5, while for compound 6 the Michael acceptor double bond from compound 4 was reduced. For cell viability assays, the IC 50 values for compounds 4, 5, and 6 against the Leishmania parasite were 7, 20, and 16 µM for the promastigote form, and 19, 11, and 1 µM for the amastigote form inside infected macrophages, respectively. EPR spectroscopy of a lipid spin label incorporated into the parasite plasma membrane demonstrated that treatment with these compounds in culture medium causes membrane rigidity in a concentration-dependent manner, owing to oxidative processes. From the IC 50 values with different cell concentrations, the membrane-water partition coefficient (K M/W ) of the compounds could be estimated, as well as their concentrations in the membrane (c m50 ) and in the aqueous culture medium (c w50 ) that reduce antiproliferative activity by 50%. Values for these biophysical parameters were similar to those reported for the antileishmanial drug, miltefosine. K M/W values indicated that the compounds have high affinity for the parasite membrane, with the compound containing the BHT group having the highest affinity. Compound 5, which contained a two 4-hydroxy-3-methoxyphenyl scaffold, showed the highest values of cw50 and cm50 . Compound 6 showed that the reduction of the double bond decreased the membrane affinity and the c w50 value, but maintained the c m50 , demonstrating the same activity as compound 4 for assays with higher concentration of cells. As cytotoxic activities were modulated by effects on the cell membrane, our results suggest that the primary action of the compounds is on the parasite membrane.
Biocurativos e suas aplicações em medicina regenerativa: uma revisão descritiva
(Universidade Federal de Goiás, 2024-01-18) Ribeiro, Camila Silva; Silva, Artur Christian Garcia da; Silva, Artur Christian Garcia da; Santos, Jordana Andrade; Passos, Maria Cláudia
Biocuratives are part of an area of research and technology that advances the development of biological materials in order to repair or regenerate tissues, generating healing in chronic and advanced injuries. These materials have excellent properties such as: biocompatibility, low toxicity, biological adaptability, sustainability, potential for customization, among others. The aim of the latest research on biocuratives is to promote effective cellular therapy, with high regenerative power, maintaining tissue function in advanced, chronic injuries or in wounds that do not heal in a timely manner, generating disruption and compromising the lives of patients. There are several targets of interest in the scientific community in clinical treatments, regenerative medicine stands out, since such biological dressings have the power to intervene in hydroelectrolytic losses, avoid bacterial contamination, favor the epithelialization of granulation tissues for grafting, being widely used in burns, chronic ulcers, reconstructive surgeries and others, being highly explored in clinical uses. The latest clinical research has demonstrated favorable results, along with histological presentations, tensiometric and histochemical properties, in the different aspects related to such materials. The objective of this review was to present the main aspects that biocuratives present regarding their use in tissue engineering, cell therapy and tissue regeneration. From this review, it was observed that future studies should emphasize the production of biological materials together with clinical trials to promote wound healing, with success.
Desenvolvimento e caracterização de carreador lipídico nanoestruturado contendo leflunomida
(Universidade Federal de Goiás, 2023-08-02) Nakamura, Juliana Midori; Silva, Luís Antônio Dantas; Salomão, Mariana Arraes; Hayasaki, Tacio Gonçalves; Silva, Luís Antônio Dantas
Rheumatoid arthritis (RA) is an autoimmune disease characterized by deformation and destruction of peripheral joints. One of the drugs indicated for its treatment is leflunomide (LEF). There are strategies that can be adopted to improve the low aqueous solubility of the drug and minimize its systemic adverse effects (gastrointestinal symptoms, allergic reactions alopecia, skin eruptions, opportunistic infections and arterial hypertension), such as nanoencapsulation and local administration. This project aimed to develop nanostructured lipid carriers (NLC) and to evaluate their physicochemical characteristics, storage stability, kinetic stability and encapsulation efficiency. The method of emulsification and solvent evaporation associated with microfluidization was used. In the physical-chemical characterization and in the stability study, the average size and polydispersity index (PdI) were evaluated through dynamic light scattering (DLS). The kinetic stability of NLC was evaluated through multiple light scattering. The encapsulation efficiency was determined using the indirect method, quantifying the concentration of free drug present in the formulation. The formation of nanometric and homogeneous particles (average size of 213 nm and PdI of 0.239) was observed. The NLC showed low backscattering variation (∆BS), less than 2%, and encapsulation efficiency of 73.3% ± 9.1. The preparation method by emulsification and solvent evaporation obtained systems with appropriate characteristics for future in vitro studies of release and cutaneous permeation of nanoencapsulated LEF.
Estratégias biotecnológicas para melhoria das características sensoriais e nutricionais dos alimentos
(Universidade Federal de Goiás, 2023-08-04) Silva Júnior, Rodrigo Soares da; Silva, Luís Antônio Dantas; Moraes, Mariana Cristina de; Silva, Aline Gomes de Moura e; Silva, Luís Antônio Dantas
Food biotechnology is a research area that develops improvement activities, as a strategy to increase the stability of the microbiological, nutritional and sensory aspects of food products, which includes the application of various biotechnological methods that aim to avoid changes, whether of enzymatic, physical, microbial or chemical origin, from the origin to the manufacture of the food. These technologies employed, benefit food in nutritional and sensory aspects. This study aims to understand and describe how different strategies can be used effectively and accurately to improve the sensory characteristics of foods, in order to promote a new perspective on it. This is a literature review, focusing on the period from 2013 to 2023, where an electronic search was carried out in the Google Scholar and Scielo databases to identify relevant studies and scientific articles. Through this research, it can be concluded that a healthy and balanced diet, along with maintaining the nutritional and sensory properties of food, has aroused great interest in the world population. Several studies with the use of biotechnology have been emerging around the world in order to identify and improve the nutritional and sensory quality of foods. These food technologies are mainly used in nutritional enrichment and disease prevention, aiming at food quality. Plants are genetically modified, aiming to add new properties with benefits to human health and, as a consequence, the food generated from these plants is preserved for a longer time, avoiding losses resulting from the supply system and the effects of seasonality that generate damage to the quality of the products. foods. In conclusion, biotechnology offers immense potential to improve the sensory characteristics of foods and satisfy consumer preferences, and by understanding and effectively applying biotechnological techniques, the food industry can drive innovation, creating products that are more attractive and in line with the demands of the market and, the search for continuous improvement of the sensory characteristics of foods is fundamental to offer satisfactory gastronomic experiences, conquer consumers' loyalty and remain competitive in the food sector in constant evolution.
Estratégias para melhora da biodisponibilidade oral da insulina: uma descrição dos desafios e das abordagens descritas na literatura
(Universidade Federal de Goiás, 2023-08-09) Silva, Marcela Amorim da; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Pascoa, Henrique; Lemes, Érick de Oliveira
Diabetes Mellitus is a metabolism syndrome characterized by a permanent increase in plasma glucose. Conventional treatment, in addition to being painful and uncomfortable, generates psychological stress due to subcutaneous perforation, which may cause side effects of hyperinsulinemia, weight gain and risks of hypoglycemia. About 45-60% of patients abandon conventional treatment due to needle phobia, and oral insulin can minimize these statistics. The main challenges of oral insulin administration are maintaining the integrity of the peptide throughout the gastrointestinal tract, effecting absorption and increasing sufficient bioavailability for its therapeutic effect. Biopharmaceutical strategies can be employed, among which are: delivery systems, enzyme inhibitors, absorption enhancers, mucoadhesive systems and chemical modifications. Some of the strategies demonstrated a significant increase in bioavailability, as well as in structure protection and increased stability, resulting in promising approaches.
Estudo e compreensão do papel da microbiota cutânea como estratégia para o delineamento de cosméticos antienvelhecimento cutâneo
(Universidade Federal de Goiás, 2023-08-09) Pereira, Dyovanna Pazini; Silva, Shirley Souza; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Silva, Andreia Luiza Pereira; Maciel, Iara Mendes
The skin is the largest organ of the human body and plays a vital role in protecting and regulating the organism. It acts as a physical barrier that shields against injuries and external agents such as bacteria, viruses, pollutants, and solar radiation. The skin also assists in regulating body temperature by acting as thermal insulation and aiding in heat dissipation through perspiration. Additionally, it serves to harbor resident microorganisms with important biological functions, known as the microbiome. Hence, the study of the microbiome, referred to as microbiomics, is on the rise. Over the years, the skin undergoes chemical and physical processes that lead to cellular breakdown, resulting in wrinkles and skin aging. The study of omics sciences is essential for developing personalized strategies to maintain or restore the functions of aging skin. The pharmaceutical professional plays a significant role in this field through research and pharmaceutical care. The objective of this work is to discuss and present the existing relationship between skin aging and the microbiome. We will explore how the skin's microbiota can contribute to the development of strategies, such as the formulation of dermocosmetic products, aimed at mitigating or reversing the damages caused by skin aging. Furthermore, the role played by the pharmaceutical professional in this context will be discussed.
Estudo e compreensão do papel da microbiota cutânea como estratégia para o delineamento de cosméticos antienvelhecimento cutâneo
(Universidade Federal de Goiás, 2023-08-09) Pereira, Dyovanna Pazini; Silva, Shirley Souza; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Silva, Andreia Luiza Pereira; Maciel, Iara Mendes
The skin is the largest organ of the human body and plays a vital role in protecting and regulating the organism. It acts as a physical barrier that shields against injuries and external agents such as bacteria, viruses, pollutants, and solar radiation. The skin also assists in regulating body temperature by acting as thermal insulation and aiding in heat dissipation through perspiration. Additionally, it serves to harbor resident microorganisms with important biological functions, known as the microbiome. Hence, the study of the microbiome, referred to as microbiomics, is on the rise. Over the years, the skin undergoes chemical and physical processes that lead to cellular breakdown, resulting in wrinkles and skin aging. The study of omics sciences is essential for developing personalized strategies to maintain or restore the functions of aging skin. The pharmaceutical professional plays a significant role in this field through research and pharmaceutical care. The objective of this work is to discuss and present the existing relationship between skin aging and the microbiome. We will explore how the skin's microbiota can contribute to the development of strategies, such as the formulation of dermocosmetic products, aimed at mitigating or reversing the damages caused by skin aging. Furthermore, the role played by the pharmaceutical professional in this context will be discussed.
Estudo morfoanatômico, variabilidade sazonal e atividade larvicida dos óleos voláteis das folhas de Campomanesia pubescens (DC.) O. Berg (Myrtaceae)
(Universidade Federal de Goiás, 2023-08-08) Costa, Henrique Margues da; Paula, José Realino de; Fiuza, Tatiana de Sousa; Ferreira, Heleno Dias; Romano, Camila Aline; Fiuza, Tatiana de Sousa; Paula, José Realino de
The aim of this study was to perform from the morpho-anatomical study, physicochemical characterization, chemical composition, seasonal variability and larvicidal activity of the volatile oils of Campomanesia pubescens (DC.) O. Berg leaves. The botanical material was collected in Hidrolândia, Goiás. Morpho-anatomical characterization and phytochemical screening were performed by conventional methods. Pulverized samples were submitted to hydrodistillation in the Clevenger apparatus and the identification of the chemical components of the volatile oils obtained was done by GC-MS. The larvicidal activity was carried out with third-stage larvae of the Aedes aegypti. The leaf blade, the main vein, and the young stem have a uni-stratified epidermis covered by a cuticle. The mesophyll and cortical parenchyma of the main vein and young stem have secretory cavities and idioblasts with crystals. The main rib contains a bicolateral bundle in an open arch. The youngstem has a discontinuous band of sclerenchyma external to the phloem cells. Most volatile oil compounds are spathulenol, caryophyllene oxide, α-macrocarpene, and z-caryophylleneIn phytochemical analysis, tannins, digitalis, flavonoids, and total phenols were detected. The content of volatile compounds was 7.36%, that of total ash was 1.77%, and that of mucilage was 3.52 ml. The volatile oil at the concentration used was inactive against Ae. aegypti larvae.The present study contributes to the taxonomic knowledge of the species and provides parameters for quality control of the plant raw material. This work represents the first description of the chemical compounds and seasonal variability of volatile oils from C. pubescens leaves collected in Goiás state.
Identificação molecular de Metarhizium sp. em adultos de Aedes aegypti infectados
(Universidade Federal de Goiás, 2023-07-28) Silva, Fernanda Cristina Soares da; Rodrigues Filho, Juscelino; Luz, Wolf Christian; Rocha, Luiz Fernando Nunes; Rodrigues Filho, Juscelino
The use of entomopathogenic fungi has been an alternative of biorational control to control Aedes aegypti. A device containing mycoinsecticide based on Metarhizium humberi IP 46 for the focal control of A. aegypti adults is in developing. The evaluation of the effectiveness of this device under field conditions can be done with the aid of sensitive methods for detecting IP 46 in diseased mosquitoes captured in the field. To standardizing a methodology to detect IP 46 DNA present in A. aegypti adults after fungal exposure, adults were exposed to IP 46 formulations under laboratory or semi-field conditions for up to 8 or 14 days, respectively. In laboratory testing, the DNA extraction buffers by Saghai-Maroof et al. (1984) (I), Raeder and Broda (1985) (II) and Doyle and Doyle (1987) (III) were evaluated. For the semi-field test, the most efficient buffer in DNA extraction was used in the treated samples. The extractions and amplifications were confirmed by electrophoresis. The TEF-intron region was amplified by PCR using primers EF1T and EF2T. For positive control, use IP 46 mycelium, and for negative control, use unexposed mosquitoes. PCR products with positive amplification were sequenced. The results of the sequencing appreciated that, with buffer I, it was possible to extract DNA from IP 46 present in A. aegypti with incubation of 0, 6 or 8 days at 75% relative humidity (RH) and on 2 or 8 days more of 98% RH. With buffer II, fungal DNA transmission occurred in mosquitoes incubated for 4, 6 or 8 days at 75% RH and for 2, 4, 6 or 8 days at more than 98% RH. IP 46 DNA was obtained from mosquitoes incubated for 2, 6 or 8 days at 75% RH and 1, 2, 4 or 8 days at more than 98% RH, using buffer III. Buffer II showed the most consistent results, so it was used in field trials. With this methodology, it was possible to detect IP 46 in live or dead mosquitoes at different chances of fungal infection. The primers used in the PCR were specific for M. humberi, with no amplification of DNA from othermicroorganisms present in the tested mosquitoes. The buffer described by Raeder and Broda (1985) (II) was the most efficient in the transmission of M. humberi IP 46 DNA in A. aegypti adults, showing potential to be used in a technique for monitoring the effectiveness of the device under conditions field. The evaluation of the protocol in semi-field samples still needs to be done in a more standardized way.
Modulação da imunidade pela microbiota intestinal: uma revisão descritiva sobre o uso de probióticos como imunorreguladores
(Universidade Federal de Goiás, 2024-01-22) Oliveira, Alexandre Martins de; Alves, Marcos Rafael de Oliveira; Silva, Luís Antônio Dantas; Silva, Luís Antônio Dantas; Alcântara, Keila Correia De; Silva, Aline Gomes de Moura e
This study sought to understand how the use of probiotics can contribute to human health. A descriptive review was conducted with the terms MALT, GALT, gut and physiology, mucosal immunity, gut microbiota, microbiota host interactions, M cells, intestinal immune cells, Probiotic, Probiotic and diseases. The main findings indicated that probiotics can contribute to human health through immunoregulation, by stimulating the production of IgA’s and modulating inflammation via TLR activation. These microorganisms also assist the human body by their ability to produce and secrete substances with antimicrobial properties, such as the 925A strain of Lactobacillus brevis. In addition to presenting the ability to competitively inhibit a wide bacterial spectrum, including E. coli, Salmonella, Helicobacter pylori, Listeria monocytogenes and Rotavirus, as is the case with strains of Lactobacilli and Bifidobacteria. It was concluded that probiotics can positively alter the dynamics of human health through immunoregulation, production of antimicrobial substances and by the competitive exclusion of pathogens. Such understandings are fundamental tools for the development of new approaches and products that explore the use of probiotics in modulating the immune system, treating pathologies and even in optimizing the efficacy of vaccines, and that more research is needed to optimize their use in clinical practice.
Presença de antibióticos na água e seus efeitos tóxicos sobre os ecossistemas e a saúde humana
(Universidade Federal de Goiás, 2023-07-28) Nascimento, Letícia Alves; Oliveira, Gisele Augusto Rodrigues de; Mendonça, Reginaldo Teixeira; Alves, Virgínia Farias; Oliveira, Gisele Augusto Rodrigues de
Medicines are developed for therapeutic purposes, but the presence of these compounds in the environment has led them to be considered emerging contaminants due to their toxicity to biota. Antibiotics have been found in various water matrices in several countries. In view of this, the aim of this study was to analyze the presence of antibiotics in water resources and their toxic effects on aquatic biota and humans. To this end, a bibliographic search was carried out using the main PUBMED, MEDLINE and GOOGLE ACADEMIC databases. The descriptors used were: antibiotics and wastewater, antibiotics and water and human toxicity, antibiotics and water sources and toxicity, antibiotics in wastewater or aquatic toxicity. A total of 150 articles were found, 134 of which were excluded as only 19 met the inclusion criteria, i.e. articles presenting data on the toxicity of antibiotics present in water resources. In terms of geographical distribution, China and Europe accounted for 31.6% of the publications on this subject. It has been observed that antibiotics are present in the most diverse water matrices: lakes, wastewater, groundwater and even drinking water. Among the main classes of antibiotics found in water resources are fluoroquinolones and sulfonamides. Malformations in aquatic organisms and the selection of resistant bacteria were the main effects observed in relation to the presence of antibiotics in water. The results of this review also indicated that the detection and quantification of these compounds in water resources depends on very sensitive analytical methods, since antibiotics are present in low concentrations. Therefore, it can be concluded that the presence of antibiotics in aquatic resources constitutes a serious public and environmental health problem and that preventive and corrective actions are necessary to minimize this source of exposure and generate subsidies for the establishment of exposure limits to these compounds in water resources.
Revisão da literatura sobre o impacto da estimulação da microbiota intestinal com prebióticos e probióticos na integridade cutânea
(Universidade Federal de Goiás, 2023-08-10) Ribeiro, Cibele Valadares; Viana, Narielly Cristina Rezende; Silva , Luís Antônio Dantas; Campos, Geovana Batista de; Chaul, Luíza Toubas; Silva, Luís Antônio Dantas
The manifestation of skin diseases is favored by several factors, among which are: genetic, environmental, psychosocial, and immunological. Considered a key regulator of the immune system, the microbiota acts in gut homeostasis. Changes in the microbiota can contribute to the development of several disease states, including diseases of the integumentary system. Aim: To analyze and present the effects of oral probiotics and prebiotics on the intestinal microbiota and their effects on the maintenance of sq1 kin characteristics, to improve the quality of life of a relevant portion of the world's population that suffers from skin problems. Method: The PubMed, Scielo and CAPES Periodicals Portal database from 2007 to 2023 was used with the following descriptors: “probiotics”, “prebiotics” “skin”, “intestinal microbiota”, “skin care” and “Skin microbiota”. The selected articles were read in full to develop the narration. Results: A healthy balance of gut microbiota is necessary for optimal skin health, resulting in metabolic and immune homeostasis, according to this narrative review. More research has linked changes in gut microbiota composition to the worsening of inflammatory skin conditions such as eczema and psoriasis. Any alteration in the diversity of intestinal microorganisms (dysbiosis) can make the host more vulnerable and interrupt the immune tolerance of the mucosa, influencing the health of the skin. As a result, a variety of dermatological conditions have been linked to intestinal dysbiosis. In the case of dysbiosis, probiotics and prebiotics should be used. Conclusion: Oral probiotics and prebiotics are beneficial in the clinical treatment of skin diseases involving immunological, inflammatory and dysbiotic factors in most of the studies addressed. Both probiotics and prebiotics can help you achieve a healthy complexion while alleviating existing skin conditions. Try them out by incorporating them into your daily skincare routine as well as your diet.
Sistemas lipídicos como abordagens tecnológicas para melhora das propriedades biofarmacêuticas de fármacos: levantamento das estratégias e mecanismos descritos na literatura
(Universidade Federal de Goiás, 2023-08-08) Carvalho, Gabriel Cruvinel; Gomes , Lara Ferreira; Silva, Luís Antônio Dantas; Morais, Mariana Cristina de; Alves, Guilherme Liberato; Silva, Luís Antônio Dantas
In the current situation, technological approaches have been sought to optimize the delivery of drugs and guarantee their therapeutic efficacy. The use of lipid systems to improve the bioavailability of orally administered drugs can help correct problems related to the physicochemical properties of the drug, such as low solubility and/or low permeability. The aim of this article was to address lipid systems as technological strategies for improving the biopharmaceutical properties of orally administered drugs. The methodology used was a survey of studies described in the literature over the last 20 years, describing the subject, and especially the benefits and challenges of using lipid systems for orally administered drugs. The theoretical framework therefore presented the parameters for the use of lipid systems as drug carriers, considering their challenges and classifications, highlighting strategies to overcome absorption problems through the use of lipid systems and the main mechanisms, as well as current applications. The technological development of lipid-based drug delivery systems therefore appears to be a promising strategy for improving the biopharmaceutical properties of drugs. With an in-depth understanding of the physicochemical characteristics of drugs, formulation techniques and the gastrointestinal system, it is possible to overcome the challenges of the applicability of lipid formulations and highlight their benefits, which will contribute to significant advances in the field of pharmaceutical technology.

Navegar

Navegando FF - Trabalhos de Conclusão de Curso por curso "Farmácia (RMG)"

Resultados por página

Opções de Ordenação