Doutorado em Ciência Animal (EVZ)
URI Permanente para esta coleção
Navegar
Navegando Doutorado em Ciência Animal (EVZ) por Por tipo de Acesso "Attribution-NonCommercial-NoDerivatives 4.0 International"
Agora exibindo 1 - 7 de 7
Resultados por página
Opções de Ordenação
Item Aspectos clínico-epidemiológicos e tratamento da tripanosomose bovina por trypanosoma vivax no estado de Goiás(Universidade Federal de Goiás, 2019-03-01) Bastos, Thiago Souza Azeredo; Linhares, Guido Fontgalland Coelho; http://lattes.cnpq.br/6261928164195145; Lopes, Welber Daniel Zanetti; http://lattes.cnpq.br/4480577038117234; Lopes, Welber Daniel Zanetti; Felippelli, Gustavo; Krawczak, Felipe da Silva; Ferreira, Lorena Lopes; Silveira Neto, Osvaldo José daThis study reports the first case of bovine trypanosomiasis caused by Trypanosoma vivax in Goiás, Brazil, as well as other cases that occurred within a period of 24 months, demonstrating epidemiological aspects such as needle sharing and commercialization of infected cows influencing the spread of this disease. Furthermore, it was observed that T. vivax was able to infect and develop the disease in calves when they were inoculated by the intradermal, subcutaneous, intramuscular or intravenous route. The presence of Anaplasma marginale latent in calves with trypanosomiasis interfer directly in the percentage of mortality of the animals. Finally, with regard to efficacy, only animals that received isometamidium were able to remain without new parasitaemia after 180 days of study. Treatment with diminazene allowed new parasitemia when reinfected 50 days after treatment and, finally, imidocarb was not effective in the treatment of bovine trypanosomosis. This is the first study that describes the presence of T. vivax, as well as its epidemiology in the State of Goiás, evaluated the infection capacity of this parasite by different pathways, clinical signs, and diagnostic methods and also evaluated the efficacy of the treatment with imidocarb, diminazene and isometamidium in cattle experimentally infected with T. vivax in Brazil.Item Avaliação histológica, microestrutural e de biocompatibilidade in vivo de cartilagens auriculares de bovino tratadas em solução alcalina(Universidade Federal de Goiás, 2021-03-05) Ferreira, Kamilla Dias; Silva, Luiz Antônio Franco da; http://lattes.cnpq.br/0446055905975647; Vulcani, Valcinir Aloisio Scalla; http://lattes.cnpq.br/9821938265591545; Vulcani, Valcinir Aloisio Scalla; Silva, Danilo Conrado; Martins, Apóstolo Ferreira; Franzo, Vanessa Sobue; Oliveira, Kellen de SousaWith the advancement of techniques for the correction of tissue defects or losses, the use of biomaterials that promote repair with a minimum of inflammatory response is sought. Previous studies carried out used different means of preparing and obtaining elastic bovine cartilage, aiming at the use as biomaterial. However, one of the major challenges related to the use of these membranes, refers to the occurrence of unwanted immunogenic reactions at the implantation site. This study aimed to characterize the physical-chemical, microstructural and histological properties of elastic bovine cartilages treated in alkaline solution and their biocompatibility in vivo. Therefore, physical-chemical analyzes were carried out to characterize this material. The thermal analyzes performed, Thermogravimetry (TGA) and Differential Exploratory Calorimetry (DSC), verified the possible changes in the material in view of the temperature variations in which it was submitted. The TGA curves showed the variation in the mass of treated and untreated auricular cartilages as a function of temperature and in the DSC the temperature of the collagen denaturation of the cartilage samples was evaluated. The analyzes showed that the treated cartilage had physicochemical characteristics similar to untreated cartilage. In the microstructural evaluations, 2D and 3D computed microtomography and confocal laser scanner microscopy were performed. Two-dimensional Micro-Ct showed that the cartilage region showed higher density in relation to the perichondrium, and the alkaline treatment was effective in decellularization, due to the presence of gaps observed in the extracellular matrix interspersed with the collagen structure. The three-dimensional Micro-Ct showed that the cartilage has less porosity and pores with a larger diameter and in the laser scanner microscopy it was noted that the treated cartilage has considerable roughness, factors that can contribute to cell proliferation and adhesion. In this study, histological processing of cartilage was also performed, demonstrating that the alkaline treatment promoted tissue decellularization, with the maintenance of the architecture of the extracellular matrix and the structure of elastic and collagen fibers. It was concluded that the alkaline treatment was efficient to promote decellularization in the auricular cartilage. The last stage of the study consisted of evaluating the in vivo biocompatibility of elastic cartilage implants treated with alkaline solution (CA) compared to polypropylene (TP) mesh in rabbits. It was found that the group (CA) had a less intense inflammatory process than the group (TP), in which the formation of a fibrous capsule around the implants was observed. It was noted in the group (CA) the presence of calcification promoted by osteoinduction of osteoblasts due to alkaline processing, which can be considered a bias of interest for further studies, involving the regeneration of bone tissues, in which the cell-type applicability observed is feasible.Item Fotoimunoinativação do alfaherpesvírus bovino 1 em sêmen(Universidade Federal de Goiás, 2020-09-29) Oliveira, Taise Maria dos Anjos; Gonçalves, Pablo José; http://lattes.cnpq.br/0515343409614452; Cunha, Paulo Henrique Jorge da; http://lattes.cnpq.br/3363890096623194; Souza, Guilherme Rocha Lino de; http://lattes.cnpq.br/1127532567066692; Borsanelli, Ana Carolina; Almeida, Luciane Madureira de; Souza, Márcio Adriano Rodrigues; Meirinhos, Maria Lúcia GambariniBovine alphaherpesvirus I (BoHV-1) poses a potential risk of infection to herds around the world, causing numerous economic losses related to both respiratory and reproductive infections. In the reproductive sphere, semen is an important source of spread of the virus to the herd. Thus, aiming at semen disinfection, photodynamic inactivation (PDI) was used as a viable alternative to conventional treatments. This technique involves the combination of a photosensitizing compound (PS), molecular oxygen and light, resulting in the generation of reactive oxygen species, capable of causing cell death by apoptosis, necrosis or autophagy. Several photosensitizing compounds are available on the market, however, new compounds are developed with the aim of improving efficiency against specific biological targets. Thus, this study evaluated the efficacy of palladium-bound porphyrins, halogenated sulfonated porphyrins, hematoporphyrins (HP) and tetracarboxy-phthalocyanine zinc (ZnPc), in vitro photodynamic inactivation BoHV-1. As well as, the development of immunoconjugates, composed by the direct conjugation between immunoglobulins (IgY anti-BoHV-1) and photosensitizers (ZnPc and HP) for PDI in semen. Among the photosensitizers tested, palladium porphyrins (dppf and dppe) at 10 μM showed better results in vitro, inactivating the virus completely with up to five minutes of irradiation. The photosensitizers HP and ZnPc were tested and proved to be safe for PDI in semen after evaluation of the parameters of quality and sperm viability. These PS were conjugated to IgY-anti-BoHV-1 and tested in semen infected experimentally with BoHV-1, being efficient for the complete inactivation of the virus within five minutes of irradiation.Item Ultrassonografia e morfometria do processo de involução dos componentes umbilicais de cordeiros sadios e com onfalopatia(Universidade Federal de Goiás, 2020-11-12) Pereira, Cristiane da Silva; Borges, Naida Cristina; http://lattes.cnpq.br/9181279951885005; Sant’Ana, Fabiano José Ferreira de; http://lattes.cnpq.br/5101695721028346; Sant’Ana, Fabiano José Ferreira de; Noronha Filho, Antônio Dionísio Feitosa; Queiroz, Paulo José Bastos; Sonne, Luciana; Ximenes, Fábio Henrique BezerraDespite the vast literature on the systemic consequences of umbilical disorders, little has been published about the use of ultrasound to diagnose omphalopathies in small ruminants. Therefore, the objectives of this PhD thesis were: (i) to describe the physiological regression of umbilical structures in healthy sheep - from their first day of life until the completion of this research - using ultrasound imagery of umbilical structures in Santa Inês breeds from natural conception (SMN) (n = 15 ), Dorper from natural conception (DMN) (n = 15) and Dorper from embryo transfer (DTE) (n = 18); (ii) to compare the evolution in the diameters of the measured structures between the two breeds (Dorder and Santa Inês); (iii) to evaluate lambs with omphalopathies - from first clinical signs, or initial sonographic changes, until the end of trials - and compare the data with those from healthy animals; and (iv) sonographically and histotologically describe the clinical cases, as well as related systemic changes such as death or recovery, of five lambs affected by omphalopathies, which were followed throughout the research, and (v) validate ultrasound as an early diagnostic method for this species. In healthy animals, all intra-abdominal umbilical structures could be evaluated in their longitudinal direction. Among all groups of structures being analysed, the urachus (P2) was the first one to finish its involution. Natural conception groups (SMN and DMN) presented similar regression, after the urachus, the umbilical vein at (P5), in days very close to regression the umbilical arteries (P3 and P4) and finally, the hepatic portion of the umbilical vein, in the positions (P6 and P7). In the group (DTE), shortly after the regression of the urachus, the regression of the umbilical vein in the abdominal (P5) and hepatic (P6) portions have followed. Subsequently, after one to two days, the involution of the portion in (P7) has taken place and, only then, around the 14th day, occurred the regression of the umbilical arteries (P3 and P4). There were no great differences in the process of umbilical involution between the breeds Dorper and Santa Inês nor between the methods of conception. We have thus concluded that healthy lambs present a pattern of umbilical structures involution in which 80% of the animals finish the process between their 12th and 14th day of age. In this research, the cases of five lambs with omphalopathies (three from the Santa Inês breed and two from the Dorper breed) were described. Out of these unhealthy animals, three lambs - which were diagnosed with omphaloarteritis by ultrasonography and later in histopathology - have died naturally. Two of them were recovered after treatment, one had omphalophlebitis and the other one had omphaloarteritis. The lesions of the umbilical structures, monitored by ultrasonography, are described in the thesis. The observation and evaluation of these five reported cases have shown that the use of ultrasonography in the evaluation of lambs which present clinical signs suggesting alterations of umbilical structures, with or without systemic involvement, is promising. The results of this study demonstrate that ultrasonography can be used in ovines for describing the regression of the remaining umbilical structures in healthy animals and that ultrasound imagery can be used in routine exams of lambs that present anomalies in their physical tests as a way of diagnosing umbilical alterations and preventing the progression of diseases caused by umbilical infections.Item Estudo macroscópico do telencéfalo, da vascularização da base e seios da dura-máter em Alouatta belzebul(Universidade Federal de Goiás, 2020-11-11) Pereira, Dayane Kelly Sabec; Pereira, Kleber Fernando; http://lattes.cnpq.br/4779531102983799; Vulcani, Valcinir Aloisio Scalla; http://lattes.cnpq.br/9821938265591545; Vulcani, Valcinir Aloisio Scalla; Franzo, Vanessa Sobué; Silva, Ana Paula Sousa Paixão Barroso da; Ramos, Luana dos Anjos; Birck, Arlei JoséPrimates of the species Alouatta belzebul, also known as howler monkeys, guariba and barbado are endemic to Brazil, of large size, slow movements and quadrupedal locomotion. The knowledge of the morphology of this species is scarce and, in some aspects, as the brain is non-existent. The objective was to describe the macroscopic anatomy of Alouatta belzebul's telencephalon, as well as the vascularization of the base of the brain, nuclei of the base and the venous sinuses of the dura mater. Twenty specimens of Alouatta belzebul were studied, where the brain gyres and grooves were dissected, the base nuclei were identified and the arterial system and venous sinuses were perfused with the injection of colored latex. The telencephalon of A. belzebul presented lysencephalic characteristics, corroborating with several other species of non-human primates and differing from genera such as Pan and Homo. The nuclei of the base were quite evident and the caudate nucleus, putamen, pale medial globe and pale lateral globe, cloister and black substance have been described, which are functionally related to the motor behavior of the species. In the analysis of the encephalization index, it was observed that the Alouatta belzebul is phylogenetically closer to Sapajus and Macaca and more distant from genera such as Brachyteles and Callithrix, showing expressive cognition and intelligence. Regarding the inclination of the central groove, it was observed that in Alouatta belzebul the upper extremity is posterior to the lower extremity, data that corroborate with man, baboons and chimpanzees and reveal a large frontal lobe when compared to Sapajus libidinosus, showing the maximum development in these primates. The arterial circle of the Alouatta belzebul consists of two vascular systems: the vertebro-basilar and the carotid system, which anastomose to close the arterial circuit. In the caudal portion of the arterial circle are the vertebral arteries and their branches: the rostral spinal artery and the caudal inferior cerebellar artery. Anastomosis of the vertebral arteries gives rise to the basilar artery. This presented an anatomical variation with the formation of a double basilar artery, called the arterial island. Nine venous sinuses were observed in the dura mater: dorsal sagittal sinus, ventral sagittal sinus, transverse sinus, straight sinus, sigmoid sinus, temporal sinus, parietal sinus, basilar sinus and cavernous sinus, with morphological similarities in the origin, path and destination of the flow blood to the internal jugular vein, helping in the venous drainage function of the brain in this species. Of the venous sinuses observed, the cavernous sinus was of considerable clinical and surgical importance in Alouatta belzebul due to its topographic arrangement next to the internal carotid artery and pituitary gland, data similar to those described for Sapajus libidinosus, Macaca fascicularis, Macaca mulatta, Papio ursinus, Cercopithecus pygerithrus and Galago senegalensis. The morphological study of the telencephalon, as well as the mechanisms of lining, arterial irrigation and drainage of the venous sinuses, generated information about the brain organization of the primate Alouatta belzebul, which had not been previously described and many of these data generate subsidies for understanding other áreas ethological investigations.Item Desenvolvimento de sorvete funcional simbiótico com polpa de cagaita (Eugenia dysenterica)(Universidade Federal de Goiás, 2021-12-17) Pfrimer, Renata Teixeira; Cordeiro, Clarice Gebara Muraro Serrate; http://lattes.cnpq.br/5073363015303780; Coelho, Karyne Oliveira; http://lattes.cnpq.br/4493179418459800; Nicolau, Edmar Soares; http://lattes.cnpq.br/9601723963736071; Nicolau, Edmar Soares; Fonseca, Leorges Moraes da; Lage, Moacir Evandro; Antunes, Veridiana de Carvalho; Cavicchioli, Valéria QuintanaBio-inspired technological innovation in ice cream manufacturing has driven the market for dairy products. In this way, increasing the nutritional density and approving ice cream, with the use of whey, buttermilk, inulin, probiotic and fruit from the Cerrado are viable alternative for the sector. The objective was the production and physicochemical, microbiological and sensory characterization of different ice creams, varying those due to the milk/cream ratio, whey and buttermilk defined by the Simplex Centroide Design, and subsequently added a probiotic strain of Lactobacillus acidophilus, inulin and cagaita pulp (Eugenia dysenterica). Nine ice creams were produced with whey and buttermilk contents, ranging from 5% to 15%, and milk/cream ratio from 70% to 90%, as stipulated by the Simplex Centroide Design. All formulations result within the standards defined by current legislation. Ice creams showed higher content of palmitic, oleic and stearic fatty acids. They stood out as formulations with the highest concentration of co-products for presenting the best nutritional composition, considering the high levels of protein, protein fractions, lipids and lactose. The high concentration of whey and buttermilk concentration, as well as the control formulations, totaling Lactobacillus acidophilus six, were increased with 0.5% of, being three with the addition of 20% of cagaita pulp and three without the addition of pulp. Subsequently, all formulations were increased with 5% of inulin. Physical-composition, microbiological, fatty acid profile, quantitative descriptive analysis, rheology, texture profile analysis and confocal microscopy were performed. All formulations result within the standards defined by current legislation. Available probiotic and symbiotic ice creams were categorized into standard and premium ice creams by fat content. Formulations with cagaita pulp presented higher acidity, moisture and lower melting speed and higher instrumental hardness. The ice creams exhibited equivalence in relation to the semiquantification of image pixels, accompanied by confocal microscopy analysis, with the quantification of lipids and proteins. Ice cream with the addition of pulp, presented higher intensity of yellow color and citrus aroma. All ice creams are categorized as probiotic foods and source count 8 Log10 CFU / g, being great vehicles to provide probiotic culture and all its benefits when consumed.Item Aspectos epidemiológicos e clínico-patológicos das poxviroses de bovinos no Estado de Goiás (2010-2018) e soroprevalência de vaccínia bovina em bovinos no Distrito Federal(Universidade Federal de Goiás, 2020-12-16) Silva, Lorena Ferreira; Cunha, Paulo Henrique Jorge da; http://lattes.cnpq.br/3363890096623194; Sant’Ana, Fabiano José Ferreira de; http://lattes.cnpq.br/5101695721028346; Sant’Ana, Fabiano José Ferreira de; Noronha Filho, Antônio Dionísio Feitosa; Sonne, Luciana; Flores, Eduardo Furtado; Borges, José Renato JunqueiraBased on the importance and in the scarce literature about poxviruses in cattle in Brazilian Midwestern, the current study aimed to determine the seroprevalence of vaccinia virus (VACV) in cattle of Distrito Federal (DF) (2015-2016) and to describe the epidemiological, clinical and pathological aspects of officialy notified cases of poxviruses in cattle of Goiás State (GO), Brazil (2010-2018). The samples used in this study from DF and GO were kindly provided by Secretaria de Agricultura, Abastecimento e Desenvolvimento Rural (Seagri-DF) from DF and by Agência Goiana de Defesa Agropecuária (Agrodefesa), respectively. In the first study, samples of sera of 312 cows from 64 herds were tested by virus-neutralizing test (VN) for bovine vaccinia (BV) antibodies. Estimated prevalences of 33.3% (herds) and 10.6% (animals) were observed. No risk factor with biological relevance was associated with seropositivity to BV. In the second study, during the evaluation period, 33 suspected cases/outbreaks of vesicular diseases in cattle were notified. Twenty-five out of these cases were confirmed as associated with poxviruses: 13 BV, 6 pseudocowpox, 5 bovine papular stomatitis and 1 coinfection (VACV and Orf virus-like parapoxvirus). Most cases occurred in the dry season of the respective years, between the months of June and September. Main lesions included vesicles, ulcers, crusts, papules and scars. These lesions affected mainly the teats and udder of dairy cows. Main lesions in calves consisted of ulcers in the mouth and muzzle. There were concomitant cases with similar lesions in humans that worked closely with the infected cattle. The results of this study demonstrate that different poxviruses (mainly BV) infect cattle in part of the Midwestern Brazil, with zoonotic potencial, mainly in dairy farms, but also present on beef herds. As a result, these diseases require vigilance by the oficial veterinary and local public health service.