Doutorado em Ciência Animal (EVZ)
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Item EXPRESSÃO DAS MAPKs EM FOLÍCULOS OVARIANOS E CORPOS LUTEOS EM DIFERENTES FASES DO CICLO ESTRAL DE BOVINOS(Universidade Federal de Goiás, 2009-08-21) FERREIRA, Mônica Rodrigues; KIPNIS, Ana Paula Junqueira; http://lattes.cnpq.br/1252262903952987; ARAÚJO, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928Bovine estrous cycle activates the pathways of MAPKs (mitogen activated protein) via oscillation of hypothalamus-pituitary-gonad axis hormones. The objective of this work was to evaluate in vivo the frequency of expression of active (phosphorylated) form, of p-MEK 1/2 and p-ERK1/2 in all types of ovarian follicles and corpora lutea in different phases of the estrous cycle of cattle. Bovine ovaries were harvested in a local slaughterhouse and the phase of the estrous cycle was determined, considering seven stages, defined as experimental groups: days 1-3 (n = 2), 4-6 (n = 5), 7-9 (n = 5), 10-12 (n = 3), 13-15 (n = 5), 16-18 (n = 5) and 19-21 (n = 5). After processing, the histological sections were subjected to immunohistochemistry using antibodies that specifically recognize the phosphorylated form of MEK 1/2 and ERK 1/2. Histological sections containing corpora lutea were also subjected to immunohistochemistry with antibodies that recognize p-BCL2 (B cell phospho lymphoma 2) and COX-2 (COX-2). The frequency of marked follicles was evaluated for the presence or absence of markup for the two cellular enzymes. The fragments of the corpus luteum were evaluated according to the scores of intensity for positive staining of the four antibodies. The frequencies of the markers studied in the follicles were compared using the chi-square. In preantral follicles, both MEK and ERK 1/2 showed high levels between 16 to 18 days. In the case of p-MEK, the greater activation of primordial follicles and primary held from 7 to 9. In secondary follicles, the less marked for p-MEK occurred in group 4 to 6. Levels of p-ERK began to rise from 13 to 15 days and subsequently decreased. Comparing the frequencies of MEK and ERK, there was statistic difference (p <0.0001) in antral follicles compared to other types of follicles. It was concluded that the intensity of activation of MAPKs MEK / ERK varies with the phases of the estrous cycle and follicular class studied. Since the corpora lutea were evaluated by analysis of variance after transforming the data, it was observed that the proteins studied showed variation in expression profile, in relation to the development, survival and death of the corpus luteum. Since COX-2, a precursor of prostaglandin, is capable of activating the pathways of MEK / ERK 1/2, it reduces the frequency of expression of cell survival factor BCL-2, leading to the corpus luteum at luteolysis. The in vivo evaluation of the activation profile of MAPKs without the use of hormonal protocols demonstrated the importance of this enzyme during the physiological reproductive process, raising new inquiries and providing opportunities to expand knowledge about the functioning of the hormone and its association with the cellular mechanisms activated by hormonesItem Potencial Antinocipcetivo Mecânico E Efeitos Sobre A Hiperalgesia Pós-Incisional Do Tramadol Em Equinos(Universidade Federal de Goiás, 2011-03-25) FRANCO, Leandro Guimarães; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; SILVA, Luiz Antônio Franco da; http://lattes.cnpq.br/0446055905975647Tramadol is an analgesic with action of opioids and non-opioid receptors, which acts by promoting activation of central inhibitory mechanisms of pain. In the last decade, there was a significant advance in research related to the use of tramadol in horses. There is evidence that the drug has significant analgesic effect in horses through mechanisms unclear. The aim of this study was to evaluate clinical and cardiorrespiratory effects and mechanical nociceptive threshold of tramadol, administered intravenously in horses. Moreover, we assessed also the effects of preemptive administration of tramadol on the clinical effects and post-incisional mechanical hyperalgesia. In the first study, seven adult horses were used in a three-way crossover study design in which tramadol was intravenous administered at 1mg kg-1, 2mg kg-1 e 3mg kg-1. Heart rate, respiratory rate, rectal temperature, systolic arterial pressure, diastolic arterial pressure, mean arterial pressure, intestinal motility, head height and behavioral change were recorded before and during 180 minutes after treatment. Mechanical nociceptive threshold from these points were averaged to obtain mean values at each time with von Frey electronic analgesiometer, applying a force in a hoof. Compared to others treatments, the horses that received 3mg kg-1 showed significant increases in respiratory rate and more pronounced behavioral changes. In horses treated with 1mg kg-1, there was a significant decrease in respiratory rate and more pronounced behavioral changes. Motility scores showed transitory decreased in horses treated with 2 mg kg-1. The tramadol did not caused changes in mechanical nociceptive threshold regardless of treatment. In conclusion, the tramadol at doses evaluated produced minimal transient side effects or clinical changes. However has no antinociceptive effect in a model of mechanical stimulus. In a second study, ten horses were divided in two groups. The horses were treated IV with a single dose of 2 mg kg-1 of tramadol (TG) or with a similar volume of sterile water (SG). Heart rate, respiratory rate, rectal temperature, systolic arterial pressure, diastolic arterial pressure, mean arterial pressure, intestinal motility, and behavioral change were recorded before and during 48 hours after treatment. Mechanical nociceptive threshold was measured by von Frey electronic analgesiometer in post-incisional model. To study, a skin incision was made over the thigh region. Compare to saline, tramadol caused transitory increased in mean and diastolic arterial pressure in first hour after treatment. The tramadol was able to decrease a remained time of primary hyperalgesia, but not prevented its appearance in compared to SG. In the global range score no changes were observed between groups along the moments.Item Conformação corporal e bioquímica sanguínea de cadelas adultas castradas alimentadas ad libitium(Universidade Federal de Goiás, 2009-12-15) GUIMARÃES, Patrícia Lorena da Silva Neves; STRINGHINI, José Henrique; http://lattes.cnpq.br/8505634095383289; BORGES, Naída Cristina; http://lattes.cnpq.br/9181279951885005; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257The obesity is a physical condition more frequent in each time in the population of pets, being able to contribute for the sprouting of systemic alterations capable to compromise the health of the animals. Ahead of the displayed one, this experiment was developed in the Veterinarian Hospital of the Federal University of Goiás, with 26 adult female dogs, castrated and without defined brand, they had been fed with commercial diet ad libitum, during 36 weeks, and had biweekly accompaniment of the nutritional state by means of clinical parameters (body condition score - BCS), morphometrics (abdominal perimeter, thoracic perimeter, thigh perimeter) and for the equations for determination of the percentage of corporal fat and index of corporal mass (IMC1=weightkg/height of withersm; IMC2=weightkg/hight of withers2 m; IMC3=weightkg/spine lengthm; IMC4=weightkg/spine length2 m). The metabolic accompaniment was carried through, monthly, by means of evaluation of the serum biochemistry (glucose, urea, creatinin, albumin, triglycerides, total cholesterol and HDL). The objective of the work was to verify the associations between the morphometrics variables and the serum biochemistries, by means of the multivariate analysis of main components. In the results, bigger association between corporal weight was perceived, thoracic perimeter and of thigh. Moreover, associations between the IMC3 and IMC4 were verified, respectively, by abdominal perimeter and BCS. The percentage of corporal fat was antagonistic to the perimeter of the thigh and corporeal weight and was associated to BCS and IMC4. Related to the serum biochemistry, associations between glucose and creatinin had been observed; and between the total cholesterol and HDL. Antagonism of the albumin with triglycerides, total cholesterol and HDL was verified. Moreover, the percentage of weight gain and the urea texts had been inversely proportional to the concentrations of glucose and creatinin. The present study allowed to conclude that the morphometric, especially the measure of thoracic perimeter and the classification of body condition score constitute efficient methods of monitorament of the corporal conformation, and also, to conclude that, dogs submitted to the weight gain for a short period of time, had been capable to develop compensatory mechanisms of keeping the homeostasis of metabolism, keeping the biochemical concentrations inside of the values of reference.Item AÇÃO DA CLORPROMAZINA EM RINS DE CÃES SUBMETIDOS À ISQUEMIA E REPERFUSÃO(Universidade Federal de Goiás, 2009-09-19) MENEZES, Liliana Borges de; ARAÚJO, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928Renal ischemia is present in different situations such as vascular or kidney surgery and also in renal transplantation. The aim of this study was to evaluate the renal function in dogs submitted to ischemia and reperfusion after chlorpromazine application. Twelve adult mongrel dogs were distributed into two groups with six animals: group A - ischemia and reperfusion without previous administration of chlorpromazine; group B - ischemia and reperfusion treated previously with chlorpromazine. Blood and urine samples were harvested in four different times: before ischemia, at the beginning of reperfusion, 120 minutes after of reperfusion and every week until postsurgical day 28 in order to check the possible ischemia/reperfusion late effect. Renal function was evaluated by clinical examination, serum urea and creatinine levels and urinary GGT activity. PU/CU and urinary activity of GGT were more sensitive for detecting acute tubular injury then routine urine examination, because these variables showed earlier alterations. It was not possible to verify the chlorpromazine protective action through urinalysis, creatinine and urea serum levels, excretion of GGT urinary and urine protein:creatinine ratio.Item Enfermidades Cutâneas Digitais Bovina: Aspectos Genéticos E Terapêuticos(Universidade Federal de Goiás, 2011-03-18) MOURA, Maria Ivete de; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; SILVA, Luiz Antônio Franco da; http://lattes.cnpq.br/0446055905975647This study has aimed do evaluate a risk factor for bovine digital dermatitis and the efficacy of 5% aqueous barbatimão extract solution as ancillary in interdigital skin incisional wound repair. One hundred bovine females, 50 Holstein and 50 Girolando, from two different farms were used for the risk factor study. Experimental units were divided into four groups: healthy Holstein cattle group (GHS) and digital dermatitis Holstein cattle group (GHL), healthy Girolando cattle group (GGS) and digital dermatitis Girolando cattle group (GGL). Ten milliliters of blood were collected from all animals and stored in anticoagulant added tubes. Samples were refrigerated and submitted to hemolytic tests for eight bovine blood systems: A, B, C, F, J, L, S and Z. The frequency of blood factors was determined and compared, in each system and each group. Data were analyzed through non parametric tests: Chi Square and Fisher s exact. The results indicated that the blood factors may contribute to the occurrence of digital dermatitis lesions, but further studies are needed for a more accurate conclusion. Thirty confined, 10 months old calves were selected in order to evaluate the efficacy of 5% barbatimão solution. A biopsy punch was introduced in each interdigital space, up to the dermis, to produce a lesion measuring one centimeter in diameter. The lesions were numbered 120 in total and named experimental units, which were divided into two groups of 60 units each: GB treated with 5% aqueous barbatimão bark extract solution and GC treated with water. Tissue repair was evaluated through macroscopic and histological findings in days three, seven, 14 and 21 of treatment. Macroscopic findings were analyzed descriptively, while histological findings were submitted to Mann Whitney test, with p<0.05. For lesions treated, GB group showed less intense polymorphonuclear cells infiltration on the first 14 days of treatment. A marked collagen deposition was also observed at day 14 while collagen alignment was more expressive on day 21. More specific studies may be necessary in order to determine if blood factors can contribute to the occurrence of digital dermatitis. Barbatiman 5% footbath solution for confined cattle helps qualitatively in modulation of interdigital cutaneous incisional wound repair, but does not avoid the extension of inflammatory phase of healing.Item ATIVIDADES ANGIOGÊNICA, ANTIINFLAMATÓRIA, CICATRIZANTE E ANTIBACTERIANA DO EXTRATO ETANÓLICO E FRAÇÕES DAS FLORES DA Calendula offIcinalis L. CULTIVADAS NO BRASIL(Universidade Federal de Goiás, 2008-09-11) PARENTE, Leila Maria Leal; BRITO, Luiz Augusto Batista; http://lattes.cnpq.br/3212353728321020; PAULA, José Realino de; http://lattes.cnpq.br/3191837532986128; PAULO, Neusa Margarida; http://lattes.cnpq.br/7949273598509043The Calendula officinalis L. is an annual herb, originating in the Mediterranean region, belonging to the Asteraceae family and well known as calendula. Displays orange or yellow flowers, which are attributed medicinal properties, with emphasis on healing activity. In this study, the ethanolic extract of flowers of C. officinalis grown in Brazil was prepared by soaking the cold and fractionated to obtain fractions of dichloromethane and hexane. The angiogenesis activity of the extract and fractions was evaluated using the model of membrane corioalantóide in embryonated chicken eggs. But the healing effect of the extract was evaluated by the model of skin wounds in rats, with assessment of macroscopic and microscopic characteristics. In this model, the ethanol extract of angiogenesis activity in the dermis of rats was also evaluated by immunohistochemistry, and measured the intensity of expression of vascular endothelial growth factor (VEGF). The in vitro antibacterial activity of extract and fractions was evaluated by determining the minimum inhibitory concentration (MIC) using the dilution plate. The ethanol extract and the fractions of calendula showed marked angiogenesis activity, demonstrated in both experimental models used. This activity was not related to the observed increase in intensity of expression of VEGF, which may be related to other pro-angiogenic factors. In the model of skin wounds in rats the ethanolic extract showed anti-inflammatory activity, demonstrated by the decrease in serous exudation of hyperemia, the deposition of fibrin and epidermal hyperplasia, besides the presence of more thin crusts and soaked in the control group. There was an increase in the amount of collagen in granulation tissue and antibacterial activity was observed, mainly on gram-positive bacteria. Still, the C. officinalis served as positive about the process of vascular neoformation and activity in healing skin wounds in rats, and also presented antibacterial activity in vitro. Thus, it is the healing effect attributed to this speciesItem Tomografia computadorizada de feixe cônico na odontologia de cães e gatos(Universidade Federal de Goiás, 2009-06-22) ROZA, Marcello Rodrigues da; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; SILVA, Luiz Antônio Franco da; http://lattes.cnpq.br/0446055905975647Eleven dogs and four cats with buccodental alterations, treated in the Centro Veterinário do Gama, in Brasilia, DF, Brazil, were submitted to cone beam computed tomography. The exams were carried out in a i-CAT tomograph, using for the images acquisition six centimeters height, 40 seconds time, 0,2 voxel, 120 kilovolts (kV) and 46,72 milliampéres per second (mAs). The ideal positioning of the animal for the exam was also determined in this study and it proved to be fundamental for performing the exam, which required a simple and safe anesthetic protocol due to the relatively short time necessary in obtaining the images. Many alterations and diseases had been identified, with extreme accuracy of the images, becoming the cone beam computed tomography as a safe, accessible and feasible imaging method which could be included in the small animals dentistry routine diagnosisItem Aspectos da resposta imune humoral e celular de bovinos naturalmente infectados com mycobacterium bovis e avaliação de vacina de subunidade protéica para tuberculose em camundongos(Universidade Federal de Goiás, 2008-08-05) SILVA, Ediane Batista da; KIPNIS, André; http://lattes.cnpq.br/4434965360286741; KIPNIS, Ana Paula Junqueira; http://lattes.cnpq.br/1252262903952987Several aspects of the bovine tuberculosis were analyzed in this study. The immunogenicity of recombinant MPT-51 (rMPT-51), Ag-85 and M. bovis-BCG were characterized in an immunosorbent assay, where 208 serum samples from positive intradermal tuberculin test (ITT) animals and 54 serum samples from ITT negative animals where analyzed. M. bovis-BCG and Ag-85 were strongly recognized by antibodies from naturally infected cattle. Additionally, the clinical status of the animals were correlated with the ITT positivity, with the specific production of IL-4 by TCD4 and TCD8 positive lymphocytes, and with nitric oxide (NO) production by macrophages from naturally tuberculosis infected bovine peripheral blood. ITT positive animals showed TCD4+IL4+ cells specific to M. bovis-BCG extract. High background levels of TCD8+IL-4+ lymphocytes were observed in ITT positive animals independent of the stimuli. When cell cultures where stimulated with M. bovis-BCG protein extract, there was no observed difference in NO production between the groups. Naturally tuberculosis infected bovine presented TCD4+IL4+ cells specific for M. bovis- BCG and a preserved NO production. Finnally, the immunogenicity of rMPT-51 use as a proteic sub-unit vaccine was evaluated in BALB/c mice, with two different adjuvants, incomplete Freund and CpG DNA. For this, mice were immunized and challenged with M. tuberculosis. Immunization with rMPT-51 antigen and either adjuvant induced, in the lungs, a migration increase of TCD5+IFN + cells specific for rMPT-51, when compared to controls (P<0.05). rMPT-51 plus CpG DNA presented a better performance among the different vaccination schemes tested, in part due to the ability of stiulate TCD5+IFN + cells and hampering the bacterial load, thus preserving the functional integrity of challenged mice lungs