Doutorado em Ciência Animal (EVZ)
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Item FLUIDOTERAPIA AQUECIDA NO CONTROLE DA HIPOTERMIA EM CADELAS SUBMTIDAS A OVARIOHISTERECTOMIA SOB ANESTESIA INALATÓRIA(Universidade Federal de Goiás, 2008-06-13) ATAYDE, Ingrid Bueno; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; ALVES, Rosangela de Oliveira; http://lattes.cnpq.br/5593911382353878; SILVA, Luiz Antônio Franco da; http://lattes.cnpq.br/0446055905975647Hypothermia is a frequent event in the per-operative period. Usually neglected in Brazil, it may lead to complications such as delay on anesthetic recovery, blood gas impairment, brain and myocardial ischemia and postoperative shivering. Prevention of hypothermia includes aspects from hospital facilities to training the nurse team. Heated fluid infusion is an aid on maintaining body temperature. A recent release of Brazilian industry, the SAF® Fluid Heating System, is a device which maintains warm the fluid to be infused, intended to reduce risks related to per-operative hypothermia. The following study aimed to evaluate clinical and electrocardiographic parameters, blood gas analysis, and serum biochemistry of female dogs submitted to elective ovariohysterectomy and infusion of 0,9% saline solution heated at 37ºC by SAF®. Thirteen female dogs were allocated in two groups: GI control (n=6), and GII treatment (n=7), in an operating room acclimatized at 22ºC. The parameters were evaluated along 90 minutes from pre-anesthetic medication. There were no significant clinical or biochemical variations; however there was group effect on the variables mean arterial pressure, urea, ALT, ALP and hypnosis time. There was no expressive variation on blood gas analysis, although some variables presented significant differences along the moments, but not between the groups. Electrocardiographic significant alterations were not evident, except for P wave, indicating atrial overload in GI. The isolated use of SAF®, on the conditions and degree of hypothermia of this study, was not enough to avoid hypothermia in female dogs submitted to general inhalatory anesthesia.Item Enzimas fibrolíticas de Humicola grisea [manuscrito]: produção, caracterização e seus efeitos sobre a digestibilidade in vitro do capim Marandu, casquinha de soja, feno de Tifton 85 e forragem de milho(Universidade Federal de Goiás, 2009-04-03) CYSNEIROS, Cristine dos Santos Settimi; FERREIRA, Reginaldo Nassar; http://lattes.cnpq.br/2555785079833283; ULHOA, Cirano Jose; http://lattes.cnpq.br/8368469162867277The objective of this work was to produce and characterize four multi enzyme complexes from the fungus Humicola grisea var. thermoidea, maintained for 96 hours at 42oC in growth media containing different carbon sources: Marandu grass; soybean seedcoats; Tifton 85 hay; and corn forage. Different amounts of cellulase, xylanase and -glucosidase enzymes were obtained depending on the different carbon sources. Cellulase presented increased activity in temperatures between 40ºC and 50ºC. The observed optimum temperature range for xylanase and β-glycosidase was from 50ºC and 60ºC. Optimum pH for cellulase activity was 6.0 when fungus growth occurred in Tifton hay, corn forage, and soybean seedcoats. When the enzyme was obtained from medium containing Marandu grass, optimum enzyme activity occurred at pH 5.5. Regardless of the carbon source, xylanase activity was higher at pH 6.0. As for β-glucosidase, optimum activity was observed at pH 5.5 for Tifton media as compared to pH 6.5 for corn and soybean containing media. For grass Marandu, the activity of the enzyme was maximum in the range 5.5 to 6.5. Cellulase produced from all growth media were maintained stable after incubation for 60 minutes at 39°C. Xylanase presented thermal stability during 240 minute incubation period at 50°C. Activity stability of β-glycosidase varied according to carbon source and presented 66.7 to 125.75% activity at 50°C for 240 minutes.Item Prevalência de dentes tratados endodonticamente em uma população de adultos brasileiros(Universidade Federal de Goiás, 2008-11-11) HOLLANDA, Augusto César Braz; ESTRELA, Carlos; http://lattes.cnpq.br/3611967334176683This cross-sectional study examined the prevalence of endodontically treated teeth in 1,401 Brazilian adults. Panoramic radiographs were selected at the Radiological Center of Orofacial Images (CRIOF, Cuiabá, MT, Brazil) between August 2002 and September 2007. Three independent endodontists discussed interpretation criteria and classified specimens according to the following data: presence of root canal treatment, which was defined as partially or completely filled canal space, regardless of whether filling ended at the radiographic apex or not; presence of intracanal post; and associated apical periodontitis. Odds ratio, logistic regression and a chi-square test were used for statistical analyses. Significance level was set at p<0.05. Of 29,467 teeth evaluated, 6,313 (21.4%) were treated endodontically. Endodontic treatment was most frequent in maxillary premolars and molars, whereas mandibular incisors showed the lowest prevalence. Most endodontically treated teeth were found in people aged 46 to 60 years (47.6%, p<0.001) and the prevalence increased with age in this age range. Females (61.9%, p<0.001) showed a higher prevalence of teeth with root fillings than males. The present study found a higher prevalence of endodontically treated teeth in a Brazilian adult population compared to the prevalence reported in epidemiological studies conducted in other countries.Item PERFIL SANITÁRIO DE BOVINOS DA RAÇA CURRALEIRO FRENTE A ENFERMIDADES DE IMPORTÂNCIA ECONÔMICA(Universidade Federal de Goiás, 2008-06-20) SANTIN, Ana Paula Iglesias; KIPNIS, Ana Paula Junqueira; http://lattes.cnpq.br/1252262903952987; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; BRITO, Wilia Marta Elsner Diederichsen de; http://lattes.cnpq.br/7605775995731168Com o objetivo de ampliar os conhecimentos sobre os aspectos sanitários de bovinos da raça Curraleiro, foi realizado o estudo comparativo entre animais Curraleiros e outras raças bovinas (Guzerá, Nelore, Girolando e Caracu), em relação a enfermidades de importância econômica e epidemiológica para pecuária. Para tanto avaliou-se a ocorrência de eventual diferença no perfil sanitário destes animais através de pesquisa de anticorpos para B. abortus, Leptospira spp, Neospora caninum, Toxoplasma gondii, vírus da leucose enzoótica bovina (BLV), herpesvírus bovino-1(BHV-1), vírus da diarréia viral bovina (BVDV) e realização de reação intradérmica para Mycobacterium bovis. As fêmeas bovinas eram provenientes de propriedades localizadas em Goiás e Tocantins e na região de divisa entre Goiás, Minas Gerais e Bahia (Trijunção). Em relação às doenças bacterianas, nenhum dos animais foi reagente para brucelose e tuberculose. Por outro lado, todas as propriedades estudadas apresentaram animais sororreagentes para Leptospira, N. caninum, T. gondii, BLV, BHV-1 e BVDV. Ao se comparar o Curraleiro com as outras raças separadamente, verificou-se para leptospirose que houve um número significativamente maior de animais sororreagentes da raça Guzerá. Em relação à neosporose os Curraleiros apresentaram um número significativamente maior de bovinos sororreagentes do que a raça Guzerá e Nelore. Resultado semelhante foi observado na toxoplasmose ao se comparar o Curraleiro com o Caracu. Para as demais enfermidades a resposta sorológica foi semelhante entre os Curraleiros e as outras raças. Para leucose enzoótica bovina ainda foram avaliados os níveis de proteínas totais, de frações protéicas, além da contagem de leucócitos e linfócitos, sendo os resultados comparados entre animais reagentes e não reagentes ao BLV, independente da raça. No entanto só foi observada diferença significativa em relação à gamaglobulina que encontrava-se aumentada em bovinos sororreagentes e os linfócitos, que apesar de apresentarem-se dentro dos padrões considerados normais estavam com níveis mais elevados nos animais soropositivos