Doutorado em Medicina Tropical e Saúde Pública (IPTSP)
URI Permanente para esta coleção
Navegar
Navegando Doutorado em Medicina Tropical e Saúde Pública (IPTSP) por Por Tipo de Defesa "Dissertação"
Agora exibindo 1 - 2 de 2
Resultados por página
Opções de Ordenação
Item Detecção e genotipagem do papilomavirus humano em amostra do colo do útero de adolescentes do Distrito Sanitário Noroeste do município de Goiânia, Goiás(Universidade Federal de Goiás, 2005-03-31) Daud, Lyana Elias Santos; Alves, Maria de Fátima Costa; http://lattes.cnpq.br/1412047788155346; Alves, Maria de Fátima Costa; Martins, Regina Maria Bringel; Villa, Luísa LinaBackground: Human Papillomavirus (HPV) infection is a strong predictor for cervical cancer development. Some HPV types are considered at high-risk and associated with pre-malignant lesions and cervical cancer. Genital HPV infection is highly prevalent in sexually active young women. Actually PGMY09/11 primer system associated with Restriction Fragment Length Polymorphism (RFLP), hibridization or DNA sequencing is one of the most sensitive methods for detecting and typing HPV-DNA in genital samples. Objectives: To detect and identify HPV-DNA genotypes in genital specimens among sexually active female adolescents from Distrito Sanitário Noroeste, Goiânia. Methods: We performed a cross-sectional study among 432 sexually active female adolescents (15-19 years), randomly selected at Distrito Sanitário Noroeste, Goiânia, and served by the Family Health Program. HPV-DNA was detected by PCR assay, in endocervical samples, using the PGMY09/11 consensus primer set. The presence of amplifiable DNA was assessed with -globin primers (PCO3/PCO4). Amplicons generated with PGMY primers were typed with the line blot assay (PGMY-line blot) and RFLP. Results: HPV-DNA was detected in 121 samples yielding a 28% prevalence (95% CI 23.8- 32.5). All samples yielded a -globin amplimer, confirming the DNA adequacy. Thirty different HPV genotypes were identified, the high risk genotypes frequently found being: HPV-16 (6,7%); followed by HPV-51 (5,1%); HPV-31 (4,6%); HPV-52 (4,2%) and HPV-18 (3,5%). PGMY-line blot test identified 54 cases of infection caused by multiple genotypes. The genotypes 16, 51, 18, 53 and 52 were the most frequently associated with cervical co- infection with multiple HPV types. Conclusions: A high prevalence of HPV-DNA was found and a broad spectrum of HPV genotypes was identified, predominantly high risk HPV genotypes. A high frequency of multiple HPV infections was found in the studied population.Item Avaliação do perfil de subclasses de IgG como fator prognóstico de gravidade de dengue(Universidade Federal de Goiás, 2014-09-22) Junqueira, Isabela Cinquini; Silveira, Lucimeire Antonelli da; http://lattes.cnpq.br/1252319967725429; Martelli, Celina Maria Turchi; http://lattes.cnpq.br/5867052489026059; Silveira, Lucimeire Antonelli da; Fonseca, Simone Gonçalves da; Nagata, TatsuyaVariations in IgG subclass concentrations are associated with numerous infectious diseases. Observations on dengue hemorrhagic fever pathogenesis suggest a high degree of immune stimulation with extensive activation of complement system preceding the onset of shock, signaling a possible role of complement activation products in endothelium damage and plasma leakage. Antibodies IgG1 and IgG3 are known to activate the complement system efficiently while IgG2 and IgG4 are weak activators. Moreover, these are the subclasses that exhibit cytophilic properties. Studies have demonstrated that antibodies in association with FcRs receptors, present on the surface of numerous cells of the organism, are involved with both, protection and dengue imunopathogenesis. In the present study, IgG subclass antibodies antigen-specific and also non DENV specific were determined in dengue seropositive patients through respectively semi-quantitative and quantitative enzyme immunoassays “in house” procedures. Serum samples of individuals participating in this study were collected in the epidemic period of october 2005 to march 2006 on dengue reference health care units in the city of Goiânia-GO. Study groups were divided as follows: 30 – dengue fever (DF); 30- dengue with complications (DCC) and 30- dengue hemorrhagic fever (DHF). Overall, it was observed an increase in total IgG1 and IgG3 in dengue more severe clinical forms, except in the age group of 25-45 years in which the highest concentration of IgG1 was observed in subjects with DF and not DCC nor DHF. As for antigen-specific subclasses, it was found an IgG1 raise and IgG3 decrease in the most severe clinical forms of the disease. It was also observed a significant difference in IgG1 and IgG3 concentration between the most severe clinical forms, DCC and DHF. DCC clinical form was characterized by a larger concentration of IgG3 DENV-specific while the clinical form of DHF was characterized by an increased amount of total and specific IgG1. The results of this study indicates that it may be a correlation between IgG subclasses concentrations, mainly IgG1 and IgG3, and dengue prognosis.