Doutorado em Ciência Animal (EVZ)
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Item Detecção dos genes spvC e prot6E e avaliação da infecciosidade de Salmonella sp. em poedeiras comerciais(Universidade Federal de Goiás, 2019-03-06) Figueira, Samantha Verdi; Rezende, Cíntia Silva Minafra e; http://lattes.cnpq.br/5841210447886226; Moraes, Dunya Mara Cardoso; http://lattes.cnpq.br/9632713129112672; Andrade, Maria Auxiliadora; http://lattes.cnpq.br/9441751521255467; Andrade, Maria Auxiliadora; Faria, Adriana Marques; Rocha, Fernanda Rodrigues Taveira; Stringhini, José Henrique; Teixeira, Weslen Fabrício PiresThe pathogenesis of Salmonella includes different factors, such as plasmidial genes, which because they are mobile genetic mechanisms can increase the bacterial genetic diversity contributing to the modification of virulence and adaptation to the hosts. The present work was developed to detect the presence of the virulence genes spvC and prot6E in different serovars of Salmonella enteric present in the metropolitan region of Goiânia and to verify the ability of these serovars to express their pathogenicity in embryonated eggs, neonates and laying hens and to cause contamination in eggs in experimental models. In Experiment 1, isolates of Salmonella Enteritidis, Gallinarum, Heidelberg, Infantis, Schwarzengrund and Typhimurium were obtained from organs of sick bird, eggs and environment of poultry chain and were investigated the presence of spvC and prot6E genes by real-time PCR. Of the 47 isolates, 15 (31.9%) were positive for the spvC gene and five (10.6%) for the prot6E gene. It is concluded that Salmonella Enteritidis, Typhimurium and Gallinarum, isolated from the bird and eggs contain the spvC genes. The prot6E gene was detected in the serovars Salmonella Enteritidis, Heidelberg and Typhimurium from egg samples. The serovars of Salmonella Heidelberg, Infantis, Schwarzengrund and Typhimurium from environmental samples do not have the plasmid genes spvC and prot6E. In experiment 2, two isolates of Salmonella Gallinarum, one positive and one negative for the spvC gene, were inoculated into embryos and neonates of laying birds to investigate whether the presence of the spvC gene in the Gallinarum serovar is able to determine embryonic mortality, affect the quality of the neonate and chick, production parameters and cause systemic disease in young birds. It was observed high mortality of the groups inoculated with Salmonella Gallinarum in alantoid route with one and 14 days of incubation, independent of the presence of the spvC gene. No embryonic mortality, changes in neonatal weight / egg weight and changes in neonatal quality were observed for birds inoculated through a 19-day in air chamber. Salmonella Gallinarum without and with the spvC gene was recovered from the heart, spleen, liver and ceca of birds inoculated in the air chamber, whereas for the oral inoculated group, the pathogen without the gene was recovered only in the ceca and the pathogen with the gene was recovered from spleen and cecum. Salmonella inoculation led to decreased weight gain and altered biometry of the heart and intestine. It is concluded that Salmonella Gallinarum is capable of causing embryonic mortality in embryos inoculated with one and 14 days in the allantoic cavity. The presence of the spvC gene in Salmonella Gallinarum does not affect embryo mortality, incubation parameters and systemic dissemination for birds inoculated in the air chamber. For birds inoculated by the oral route, the presence of the spvC gene determines enteric and systemic infection, whereas the isolate without the gene remains restricted to the cecum. In Experiment 3, the Salmonella Heidelberg isolate positive for the prot6E gene in experiment 1 was inoculated in laying hens in the oral, intravaginal and intravenous routes in order to investigate their ability to cause clinical disease, egg and gastrointestinal tract contamination. The pathogen was isolated in the excreta, only 12 hours after inoculation in 33% of the birds inoculated from oral route and 66% of the birds inoculated intravaginal route. In the eggs, the pathogen was isolated at 24 and 48 hours, seven and 15 days. It is concluded that the presence of the prot6E gene in Salmonella Heidelberg is not sufficient to cause clinical salmonellosis in laying hens, but it determines contamination of the eggs and the gastrointestinal tract of chickens, regardless of the route of inoculation, oral, intravaginal and intravenous.Item Efeito da fotobiomodulação e de células-tronco derivadas do tecido adiposo na reparação de feridas por queimadura térmica em ratos(Universidade Federal de Goiás, 2020-07-31) Ribeiro, Maisa; Moraes, Julia de Miranda; http://lattes.cnpq.br/7729197454739642; Vulcani, Valcinir Aloisio Scalla; http://lattes.cnpq.br/0967468447941793; Vulcani, Valcinir Aloisio Scalla; Prado, Rodrigo Paschoal; Araújo, Gustavo Henrique Marques; Saturnino, Klaus Casaro; Rita, Ricardo de Mattos SantaIntroduction: Injuries caused by full-thickness burns are considered a severe form of trauma with a high worldwide morbidity and mortality rate capable of promoting destruction of skin tissue and morphologically compromising the organism. The use of stem cells derived from adipose tissue (ADSC) and low-level laser (LLL) are promising therapeutic alternatives explored by regenerative medicine. The aim of this study was to establish an effective isolation model for more homogeneous ADSC cells; to evaluate the effects of ADSC and LBP on the skin healing process and to investigate the association of techniques (LBP and ADSC) in the treatment for full-thickness burn in an experimental rat model. Material and methods: The obtaining of the ADSC was carried out by means of imonumagnetic isolation by CD49d protein and conventional culture. A total of 150 Wistar rats were divided into a control group and groups treated with ADSC CD49d positive, ADSC CD49d negative, conventionally isolated stem cells (CULT), LLL, ADSC+LLL, ADSC-LLL. Scald burns were induced on the back of each animal, with subsequent surgical debridement. LLL with a wavelength of 660 nm, 30 mW of power at five points of the wound was used, under total fluence of 450 Joules / cm2 and total application of 5 Joules. Macroscopic and histopathological analyzes were performed according to biopsies collected on days 5, 14 and 21 post-injury, processed and stained using the techniques of Hematoxylin and Eosin (HE) and Gomori's Trichrome (GT). Statistical analyzes were performed using Tukey's test and analysis of variance (ANOVA), Kruskal-Wallis test (non-parametric) and T-Paired test. For all analyzes, a significance level of 5% (p <0.05) was used. Results: The results showed a statistical difference in morphometry between the groups with the best degree of reepithelization and reduction in the lesion area presented by the ADSC+ and CULT treatments at 21 days of biopsy. Histopathological analysis showed that ADSC+, LLL and ADSC+LLL treatments significantly influenced the healing process, showing improvement in the increase of mononuclear inflammatory infiltrates, greater angiogenesis, decreased edema, increased granulation tissue, in the number of fibroblasts at the beginning of tissue repair, in addition to promoting re-epithelialization in a more organized manner with greater deposition of collagen and formation of epithelial attachments at 21 days after the injury. Conclusion: low-power laser photobiomodulation of ADSC cells enhanced the repair process in full-thickness burn wounds, demonstrating a significant interaction for the remodeling of the extracellular matrix and skin tissue. Additional studies are needed to measure the effects of LBP and the activity of ADSCs on skin tissue repair.Item Efeitos dos extratos de folhas de campomanesia adamantium e hymenaea martiana hayne sobre células de osteossarcoma canino e células endoteliais humanas(Universidade Federal de Goiás, 2020-02-28) Vieira, Vanessa de Souza; Cruz, Vanessa de Sousa; http://lattes.cnpq.br/1222753642148629; Araújo, Eugênio Gonçalves de; http://lattes.cnpq.br/391977757005992; Araújo, Eugênio Gonçalves; Pfrimer, Gabriel de Abreu; Pereira, Kleber Fernando; Arnhold, Emmanuel; Oliveira, Gerlon de Almeida Ribeiro deOsteosarcoma (OSA) is a very aggressive tumor in dogs, with low survival rate and ineffective treatment. In the search for alternative sources of therapy, the Brazilian biome becomes a scientific hope, presenting a diversity of medicinal plants of popular knowledge and use. However, they can have controversial biological functions. The aim of this study was to verify the bioactivity of the leaves of Campomania adamantium and Hymenaea martiana Hayne on canine osteosarcoma (OC) cells from cell cultures and human umbilical vein endothelial cells (HUVEC). As cells were cultured and subjected to treatment with C. adamantium (1 μg / mL, 10 μg / mL, 100 μg / mL, 1000 μg / mL) and H. martiana Hayne (10μL / mL, 100μL / mL, 1000μL / mL , 2000μL / mL and 5000μL / mL), in the 24h, 48h and 72h exposure periods in normoxia and 24h in cells exposed to oxidative stress, induced by hydrogen peroxide. The results were analyzed by analyzing cell viability and cytotoxicity using the tetrazolium reduction method (MTT). This study brings unprecedented results in relation to extractive effects, as it was possible to demonstrate that they have little cytotoxic action. In addition, it was observed that the higher the dosage and the longer the exposure time, the greater the proliferative activity, with increased cell viability in the 72-hour group. In endothelial cells, similarly, there was no cytotoxic activity, in addition to the occurrence of increased cell viability, even after submission to oxidative stress. C. adamantium leaf extract increased viability and did not show cytotoxic action in canine osteosarcoma cells and endothelial cells under oxidative stress. The crude ethanolic extract of the leaves of Hymenaea martiana Hayne, increased the cell viability of canine osteosarcoma cells and human umbilical vein endothelial cells, submitted to oxidative stress.