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Item Abordagens médica e odontológica da neuralgia trigeminal(Universidade Federal de Goiás, 2014-07-18) Tacon, Kelly Cristina Borges; Ribeiro-Rotta, Rejane Faria; http://lattes.cnpq.br/9466795079344876; Marcelo, Vânia Cristina; Ribeiro-Rotta, Rejane Faria; Marcelo, Vânia Cristina; Brasil, Virginia Visconde; Pereira, Edna Regina Silva; Parreira, Samara Lamounier SantanaIntroduction: Among the neuropathic pain to trigeminal neuralgia (TN) belongs to the group of chronic painful conditions of the head and neck region, characterized by paroxysmal and recurrent attacks of excruciating and sudden pain, shock-like. Various specialties may be involved in the process of differential diagnosis and treatment, and the lack of integration of this process a major cause of the complexity of diagnosis and lack of treatment effectiveness. There are few specialized services to treat this type of chronic pain and the knowledge about the existing approaches of those is of fundamental importance for stimulating the process of integrating them, the translation of accumulated knowledge to other professionals and creating new ones. Objective: To investigate the medical and dental approaches front of trigeminal neuralgia in two at a public university services. Methodology: An exploratory study, cross-sectional, qualitative. The subjects were 19 professionals working in one of the services studied. The data collection instrument was an interview guide with 13 questions divided into four axes, based on literature: a) pathophysiology and diagnosis of TN; b) knowledge and use of different treatments; c) professionals' perception of the services and d) routing flows and monitoring of patients. The taped interview, open and semi-structured guided the construction of a descriptive analysis that enabled the identification of emerging analytical categories (Bardin, 1995). Results: Of the analyzed statements seven categories. Among the main results are: lack of consensus among professionals about the pathophysiology; the diagnostic process was considered eminently clinical, while further tests are requested for differential diagnosis and exclusion of secondary causes for TN; perceived lack of training for the diagnosis, particularly for dental professionals; the choice of treatment is associated with vocational training, and the reported pharmacotherapy as the most used; integrative and complementary practices are known as part of the TN treatment, reported by some use; the limitations found in the treatment included the professional integration of disabled and knowledge to suit the individual needs of patients and also those found in the Unified Health System (UHS); as the perception of the services, despite reporting poor infrastructure, understand that there are other more significant problems such as those related to human resources; lack of specific nuclei for the treatment of pain; the teaching-learning environment as a facilitator for the care and multiprofessional and multidisciplinary approach; the lack of effectiveness of previous treatment and the limitations of the UHS regulation system as increased demand generators in the investigated services; the lack of understanding on the part of patients, that healing does not occur when pain control achieved by therapeutic dose, makes it difficult to follow. Considerations: The investigation of medical and dental approaches against TN in both investigated services identified aspects that can motivate and closer to them and the enhancement of existing resources, creating more favorable results to affected individuals, vocational training, research and the solvability of UHS actions.Item O acesso a medicamentos pela via administrativa no setor público de saúde no Brasil(Universidade Federal de Goiás, 2015-05-08) Soares, Amanda Queiroz; Provin, Mércia Pandolfo; http://lattes.cnpq.br/1809891256443044; Amaral, Rita Goreti; http://lattes.cnpq.br/3665611660713029; Amaral, Rita Goreti; Dâmaso, Andréa Homsi; Andrade, Eli Iola Gurgel; Medeiros, Marcelo; Contezini, Silvana Nair LeiteAlthough the Brazilian public healthcare system is based on the principles of the universality and integrality of access to health services, severe problems remain with respect to guaranteeing access to pharmaceutical drugs. This situation has contributed to the creation of new institutional frameworks, including the administrative channel, institutionalized by the executive power with the aim of meeting the repressed demand for pharmaceutical drugs not obtained within the public health service. This study analyzed access to pharmaceutical drugs through the administrative channel within the Brazilian public health sector. As part of this larger study, various methodologies were applied, including a case-control study, a descriptive study and an interpretive case study in which the focal group technique was used. The study was developed in the town of Goiânia, in Brazil’s mid-western region. General users of primary healthcare units (or their accompanying persons), users specifically requesting pharmaceutical drugs through the administrative channel and users who had successfully obtained pharmaceutical drugs through this channel participated in the study. Results show that administrative demands for access to pharmaceutical drugs are inserted within a multifactorial context that involves users’ economic and sociodemographic characteristics, as well as the characteristics of healthcare, pharmaceutical care and healthcare conditions. Furthermore, users who had gained access to at least one pharmaceutical drug requested through the administrative channel were found to be predominantly those who were less socioeconomically vulnerable, those with a positive self-evaluation of their state of health, those requesting insulin analogs and individuals who in general did not consult or obtain medication within the public healthcare sector. The steps taken by most of the individuals who had their request approved reveal their fluidity between the public and private healthcare sectors, characterizing their efforts to guarantee healthcare. Therefore, the administrative channel was found not to be free from the barriers to achieving access to pharmaceutical drugs that exist within the public health sector in view of its interdependency on a system with shortcomings both within the health sector itself and at higher levels within the system.Item Adaptação marginal de cimentos à base de silicato de cálcio à parede dentinária de cavidades retrógadas(Universidade Federal de Goiás, 2019-07-05) Renovato, Sara Rodrigues; Silva, Julio Almeida; http://lattes.cnpq.br/1022830186974104; Estrela, Carlos; http://lattes.cnpq.br/3611967334176683; Estrela, Carlos; Alencar, Ana Helena Gonçalves de; Decurcio, Daniel de Almeida; Pécora, Jesus Djalma; Siqueira, Patrícia Correia deObjective: To analyse the marginal adaptation of calcium silicate-based cements in root-end cavity by SEM. Material and Methods: Fifty bovine roots were prepared with K-File #60 and filled with gutta-percha and MTA Fillapex. Roots were apicetomized and a 3-mm-deep root-end cavity was prepared using ultrasonic tips. Samples were randomly divided into five groups (n = 10): 1. Mk Life bioceramic cement; 2. Biodentine®; 3. Bio-C Repair®; 4. Endosequence® BC RRM™; 5. MTA Angelus® (MTA) - positive control. Root-end cavities were filled with the materials prepared according to the manufacturers’ instructions. EDTA was used prior the retro-filling material insertion in half of the samples from each group (n = 5). Samples were prepared for SEM and photomicrographs were taked in x40, x150 and x500. The images of root-end fillings were divided into four áreas and distributed into five scores: 0 - absence of gaps; 1 - presence of gap in 1 area; 2 - presence of gap in 2 areas; 3 - presence of gap in 3 areas; 4 - presence of gaps in 4 areas. The analysis of transverse dimension of the gap (μm) was performed using Image J software. Qualitative variables were evaluated by Fisher Exact Test and quantitative variables by Analysis of Variance (ANOVA) . The level of significance was 0.05%. Results: No gaps were observed in MTA Angelus® group (0%). Presence of gaps were observed in Mk Life bioceramic cement (90%), Biodentine® (80%), Bio-C Repair® (100%) and Endosequence® BC RRM™ (80%). Significant difference was observed when MTA were compared to Mk Life bioceramic cement, Biodentine®, Bio-C Repair® and Endosequence® BC RRM™ (p <0.05). No significant difference were observed in the transverse dimension of the gap between Mk Life bioceramic cement, Biodentine®, Bio-C Repair® and Endosequence® BC RRM™ groups. MTA show significantly better result than Mk Life bioceramic cement and Bio-C Repair® groups (p <0.05), but without significant difference with Biodentine® and Endosequence® BC RRM™ Conclusion: MTA Angelus® showed better marginal adaptation compared to MK Life bioceramic cement, Biodentine® , BioC Repair® and Endosequence® BC RRM™. Marginal gaps were not observed in samples of MTA Angelus® group.Item Adenovírus humano em água tratada e avaliação da sua recuperação em solução com sólidos tropicais(Universidade Federal de Goiás, 2013-10-10) Silva, Hugo Delleon da; Anunciação, Carlos Eduardo; García-Zapata, Marco Tulio Antonio; http://lattes.cnpq.br/3672512339058369; García-Zapata, Marco Tulio Antonio; Tada, Mauro Shugiro; del Aguila, Nora Katia Saavedra; Fiaccadori, Fabiola Souza; Souza, Guilherme Rocha Lino deHuman adenovirus (HAdV) is indicated as a viral biomarker of water quality. Thus, studies that show or even the occurrence of these interactions in water are of great importance, since these studies are still scarce. The aims of these studies were: (i) to detect, quantify and molecularly characterize the HAdV serotypes that can to circulate in the water supply treatment in the city of Goiania; (ii) evaluate the infectivity and recovery of genomic copies (GC) of HAdV-5 in simulated condition with solids derived from tropical soils and under controlled conditions of the pH and the presence of organic matter (OM); (iii) establish a mathematical equation to evaluate the recovery rate of virus genome copies in simulated conditions with clay soils. Thus, in the second half of 2012, we collected sample water in a volume of 5 L of 4 treated water reservoir and their respective points in the distribution network in the city of Goiania, totaling 80 samples. The samples were concentrated, quantified by qPCR and sequenced. Altogether 76.6% (100 - 109 CG/mL) and 37.5% (101 - 108 CG/mL) of the samples were positive for the reservoir and their respective points in the distribution network respectively. Therefore, Goiânia’s treated water is contaminated with a high number of HAdV type C. In the study of the interaction of HAdV-5 with the solid (sediments), a hydromorphic soil sample was divided into two parts: soil organic matter (WOM) and soil less organic matter (LOM). Then, it was added separately 5, 25 and 50 mg of soil WOM and LOM in sterile polypropylene tubes of 50 mL, added ultrapure water and adjusted the pH to 6.0 and 8.0. Then was added 1 mL of viral aliquot and the volume made up to 50 mL. The tubes in replica were shaken at 150 rpm for 1h at 24 °C followed by decantation. The viral genome was quantified (GC/mL) by Real-Time PCR and the Infectivity by Assay Plate Lysis. Water with solids promoted a reduction in the number of GC/mL and viral infectivity. The OM did not affect the recovery of GC/mL (p> 0.05). However, the OM was harmfulto to the infectivity of the virus, with a reduction of 2 log10 of Plaque Forming Units per milliliter (PFU/mL), when compared with treatments LMO. The acidic pH is unfavorable to virus inactivation, and the clay is the main element responsible for the interaction of HAdV-5. The mathematical equation is useful in assessing the recovery of viral genomic copies in clay solutions. These data can offer support in ecoepidemiological studies of viral inactivation or water treatment.Item Adesão de pessoas adoecidas de AIDS (Síndrome da Imunodeficiência Adquirida) à terapia antiretroviral: estudo clínico e laboratorial à partir da orientação de Enfermagem(Universidade Federal de Goiás, 2011-02-21) BRASILEIRO, Marislei de Sousa Espíndula; CUNHA, Luiz Carlos da; http://lattes.cnpq.br/6349547031976679Adherence to antiretroviral therapy reduces the risk of treatment failure, toxicity and resistance, so the therapeutic monitoring is necessary in the treatment of infection by human immunodeficiency virus. The aim of this study was to determine, through clinical and laboratory studies, the compliance of individuals sickened by HIV / AIDS with antiretroviral therapy before and after three nursing interventions. The study design was analytical, descriptive, prospective and quantitative, using data from medical records, interviews and blood sampling for plasma levels of efavirenz by HPLC analysis, including subjects with a diagnosis of HIV / AIDS, attended at the Hospital for Diseases Tropical Goiânia - Goiás and treated with zidovudine/ lamivudine and efavirenz. The results of analysis of data from 15 subjects indicate a socio-demographic profile, predominantly male, young, heterosexual. After nursing intervention, there was a change in 48% of the diagnoses. We also noticed a positive change in the rates of CD4, corresponding to 14%. As viral count, an increase of subjects with undetectable loads of 1 st to 2 nd nursing intervention (20%, or 73.3% to 93.3%). Chromatographic analysis of plasma of the subjects indicated that 60% of them had adequate medication adherence, and 40% increased adhesion between the 1st and 2nd visits by nurses, 10% between the 1st and 3rd and 10% between the 2nd and 3rd queries. The factor that was associated with high concentration rate after adjustment in nursing diagnoses and prescriptions was having difficulty using the antiretroviral in the same time. It was also demonstrated significant difference between mean plasma concentrations of efavirenz from the 1st, 2nd and 3rd interventions. Considering the reference measurement of plasma concentrations of efavirenz, the method of accession had a sensitivity and specificity, with high proportion of agreement between the rate of CD4, nursing diagnosis and analysis of the plasma of subjects. Conclusions: the systematization of nursing care and determination of plasma concentrations should be incorporated into the routine outpatient care, for better monitoring of adherence to antiretroviral therapy of individuals sickened by HIV / AIDS.Item Alterações dentofaciais em meninas com puberdade precoce(Universidade Federal de Goiás, 2016-12-22) Paula Junior, Delcides Ferreira de; Mendonça, Elismauro Francisco de; http://lattes.cnpq.br/2305019128015847; Leles, Claudio Rodrigues; http://lattes.cnpq.br/6740286066154410; Leles, Claudio Rodrigues; Mendoça, Elismauro Francisco de; Roriz, Virgilio Moreira; Dias, Danilo Rocha; Castro, Anelise Daher VazThe objective of this study was to evaluate the prevalence of malocclusion, the need for orthodontic treatment and dentofacial changes in girls with Precocious Puberty (PP). This work gave rise to two distinct studies and data analyzes including descriptive statistics, Pearson's correlation analysis, paired t-test and t-test for one sample: 1- the cross-sectional study included 39 girls (6 to 11 years old) with confirmed PP diagnosis. The Dental Aesthetic Index (DAI) and the Index of Orthodontic Treatment Need (IOTN) were used for malocclusion evaluation and need for orthodontic treatment. Cephalometric analyzes were performed for the diagnosis of facial growth abnormalities. There was a high prevalence (64.1%) of severe and very severe malocclusion (DAI grades 3 and 4) and 82.1% of cases requiring moderate to high treatment (IOTN grades 3 and 4). 2- the control case study included 39 girls (6 to 11 years old) with confirmed PP diagnosis (case group). In this group, dental panoramic and hand and wrist radiographs were made. The control group consisted in 78 panoramic radiographs of girls without PP, randomly selected. Each panoramic radiograph of the 39 from case group was compared with two radiographs from control group, that is, a ratio of 1: 2. The chronological chart of dental mineralization of Nicodemus was used to identify the dental age. A significant difference (p <0.001) was found between chronology and bone age. Dental age (mean = 117) was lower than bone age (mean = 123) in girls with PP, but higher than dental age (mean = 111.5) in the control group. There was a significant correlation between chronological age and dental age. It was concluded that PP can have an impact on the development of dentition and craniofacial growth, a relevant factor for the diagnosis and the choice of the best moment for orthopedic / orthodontic intervention in malocclusion.Item Alterações metabólicas, qualidade de vida e capacidade funcional de idosos atendidos na estratégia de saúde da família de um munícipio de Goiás(Universidade Federal de Goiás, 2016-05-27) Avelar, Ivan Silveira de; Silva, Maria Sebastiana; http://lattes.cnpq.br/1329422634395496; Silva, Maria Sebastiana; Souza, Nilva Pessoa de; Campos, Mário Hebling; Nora, Fernanda Grazielle da Silva Azevedo; Gassi, Ewerton RodrigoAging involves neural structural, functional and chemical changes that generate a number of diseases. Among the diseases that affect the elderly stand out metabolic and functional, such as metabolic syndrome and loss of muscle strength, which are closely related to the quality of life and are influenced by inadequate nutritional status and physical inactivity. Studies show that protein supplementation can increase muscle strength and improve body composition. The objective of the study was to evaluate the metabolic changes and the quality of life as well as the effects of a water aerobics program associated with supplementation of soy protein in muscle strength, hydration status and body composition of the elderly. The research began with the participation of 63 elderly aged ≥ 60 years. We determined the frequency of metabolic syndrome and assessed the quality of life of those with and without SM, using the SF-36. Still, the elderly were subjected to a water aerobics program associated with supplementation of soy protein for six months and evaluated for manual pressure force by force transducer manual, and state hydration and body composition by BIA. The normality of the data was assessed by the Shapiro-Wilk. The Mann-Whitney test was used to compare the variables of MS and the scores of quality of life between the groups with metabolic syndrome (CSM) and without metabolic syndrome (SSM). The scores of each SF-36 domain were divided into tertiles and associated with individuals with and without MS using the chi-square test of Pearson (2). In the study evaluating the effect of water aerobics program with or without supplementation used the Student t test for independent samples to compare the variables with normal distribution between the groups and the Mann-Whitney test to compare variables without normal distribution. In the above analysis we used the SPSS software and adopted the significance level of p <0.05. The vectors of BIVA were analyzed by Hotelling's T2 tests and univariate analysis (F test). The distances between the vectors in each group were correlated, was also calculated and, using Mahalanobis distance D (D). The vectors were analyzed using the software BIVA 2002. The effect of supplementation on strength and body composition was assessed by analysis of variance (ANOVA) for repeated measures with Bonferroni post hoc. The results indicated a prevalence of 79.03% of elderly patients with metabolic syndrome. There was no difference between the domains of quality of life among older people with and without MS. qualitative improvement was observed in the above hydration. The study indicated a high frequency of metabolic syndrome but did not affect the quality of life of the studied group. The manual force had a significant increase (p=0.001) in the group that underwent aquatic exercise performed and consumed the soy protein supplement. Fat-free mass was significant loss in the group that used the protein supplement. The practice of gymnastics, with or without supplementation increased the manual pressure force, but did not change the body composition of the elderly.Item Alterações pulmonares espirométricas e tomográficas após exposição à fumaça da combustão de lenha(Universidade Federal de Goiás, 2011-05-05) MOREIRA, Maria Auxiliadora Carmo; BARBOSA, Maria Alves; http://lattes.cnpq.br/5025797873585225Introduction: Exposure to smoke from wood combustion contributes significantly to the prevalence of COPD in many countries. Findings from studies in other countries are not fully applicable to Brazil due to the diversity of vegetation used as firewood, the almost exclusive use of firewood for cooking, and limited use of other types of biomass in Brazil. The importance of high-resolution computed thorax tomography (HRCT) as an adjuvant tool to complement spirometry for COPD diagnosis has increased and studies of diseases caused by wood smoke are still scarce. Objectives: Identify women with COPD and a history of wood smoke exposure and to characterize this group in terms of exposure and clinically, demographically, spirometrically and tomographically. Particularly in regard to tomography, the goal was to add to the limited literature on this subject, both in Brazil and internationally. Methods: One hundred sixty female non-smokers exposed to smoke from burning wood and 31 normal controls with no history of exposure to wood smoke were included in the study. Through a validated questionnaire, demographic and clinical data (respiratory symptoms) and information about environmental exposures were obtained. Spirometries were carried out on all the women and the lung volume of those with COPD was measured. Forty-two patients with COPD and all of the control group underwent HRCT.Item An€álise da resposta de Paracoccidioides brasiliensis a diferentes tipos de agentes estressores e osmoreguladores e express o heteró‚loga e localizaçムo de β-1.3-glicana sintase(Universidade Federal de Goiás, 2010-02-26) TOMAZETT, Patrícia Kott; PEREIRA, Maristela; http://lattes.cnpq.br/1345781867765758Paracoccidioides brasiliensis is a thermo-dimorphic human pathogenic fungus that lives at 23”C in the mycelium phase (infecting phase) and at 37”C in the yeast phase (parasite phase). In attempt to survive, the cell wall of fungi can change its composition and/or structure in response to environmental stress by compensatory mechanisms. The molecules involved in these mechanisms are possible target for the development of effective antifungal agents. In P. brasiliensis, the main components of the cell wall are glucans and chitin polymers. These polymers make a primary barrier that is responsible for the structural integrity and form of the cell wall. In this work the behavior of P. brasiliensis was evaluated against stress conditions with the aim of study, for the first time, the mechanisms used by this fungus in the maintenance of the cell wall integrity. Our results shown that P. brasiliensis yeast cells are sensitive to cell wall stressors calcofluor white (CFW), congo red (CR), SDS, KCl, NaCl and sorbitol. There was an increase in the PbFKS1 transcripts expression and in the content of cell wall β- 1,3-glicana after treatment with all stressor agents. After treatment with SDS and KCl the PbGFA1 transcripts expression and the cell wall GlcNAc residues also increased. The transcript expression of PbGEL3 was also evaluated being increased after treatment with CFW, NaCl and sorbitol. Thus we showed that these molecules are involved in the maintenance of the cell wall against stress conditions. Apart from these analyses we obtained the active recombinant protein PbFks1pc. Through the anti-PbFks1pc antibody we performed immunocitolocalization assays. These experiments revealed the localization of PbFks1p in regions of apical growth in the mycelium phase and in the cell surface in yeast phase.Item Análise bioquímica das alterações salivares relacionadas à adaptação funcional de pacientes usuários de próteses totais(Universidade Federal de Goiás, 2014-12-18) Breseghelo, Maria de Lourdes; Guillo, Lídia Andreu; http://lattes.cnpq.br/3401436781775091; Leles, Cláudio Rodrigues; http://lattes.cnpq.br/6740286066154410; Leles, Cláudio Rodrigues; Ferreira, Reginaldo Nassar; Barcelos, Bento Alves de; Cardoso, Clever Gomes; Paula, Juliê Marra deComplete dentures represent the main treatment for total edentulism in the overall population. However this type of treatment can lead to the onset of physiological and functional alterations during the adaptative period after the installation of the denture. The objective of this work was to evaluate modifications in biochemical parameters (nitric oxide and cortisol levels) in complete denture user's saliva and verify its association with clinical parameters like levels of functional adaptation, trauma, salivary flux, and viscosity. Nineteen totally toothless patients in need of installation or substitution of dentures were selected and evaluated during the installation, adaptation and in the post-adaptation period. The protocol for making the complete dentures followed clinical and laboratory procedures. The nitric oxide concentration dosage was performed via the Griess reaction, where the measurement taken is the nitrite concentration, since nitric oxide is very unstable and rapidly transforms into nitrite. The points for construction of the calibration curve were made in triplicate using the nitrite concentration, which varied from 5 to 150 μM, wherein for each analyzed plate a new curve was built. The salivary cortisol concentration was performed using Salimetrics No. 1-3002 (single) enzymatic kit. A descriptive statistical analysis was done for representation of summary measures of the variables measured. Non-parametric tests (Friedman, Wilcoxon, Mann-Whitney and Kruskal-Wallis tests) were used for group comparison. Salivary nitric oxide levels were quantified for each evaluation period and correlated between themselves, showing statistically significant differences among them. The adaptative periods showed a decrease in nitric oxide levels between installation and post-installation. There were no significant differences in correlations between salivary nitric oxide concentrations and levels of functional adaptation, trauma, salivary flux and viscosity. The salivary cortisol levels were evaluated at two periods, in the post-adaptative and after some months of denture use, showing great variation at the first period and stabilization at the second. There was no significant difference when correlated to the levels of functional adaptation and trauma. It was concluded that the prosthetic treatment caused alterations in salivary nitric oxide and cortisol levels during adaptation to dentures however these levels came back to normal after that period.Item Análise clínica e microbiológica de úlceras venosas de pacientes atendidos em Unidades Básicas de Saúde de Goiânia(Universidade Federal de Goiás, 2012-03-30) SANTOS, Silvana de Lima Vieira dos; BACHION, Maria Marcia; http://lattes.cnpq.br/8503907944360635This cross-sectional study was performed in the dressing rooms of the primary healthcare network of Goiânia, Goiás, Brasil, with the following objectives: to identify the prevalence of Gram-negative bacteria (GNB) in venous ulcers (VU) with clinical signs of infection; analyze the susceptibility profile of the isolates; detect the production of AmpC β-lactamases and metallo-beta-lactamases, and extended spectrum beta-lactamase (ESBL); describe the clinical signs and symptoms of infection in VU; evaluate the wounds clinical stage of infection and its relationship with the presence of GNB. The data were analyzed by means of descriptive statistics procedures, proportion and Chi-square tests (p<0.05). All ethical aspects were followed. The participants were 69 patients with venous ulcers, with or without arterial complication, totaling 98 wounds. It was verified that 74.5% of the wounds showed GNB growth, particularly enterobacteria (53.8%) and non-fermenting gram-negative bacteria (46.2%). The prevalent species among the enterobacteria was Escherichia coli (24.5%), followed by Enterobacter aerogenes, Pantoea agglomerans and Proteus mirabilis (12.2% each). Regarding the non-fermenting gram-negative bacteria, the prevalent genre was Pseudomonas (66.6%), particularly the species P. aeruginosa (59.5%), present in 25.5% of the analyzed wounds. Regarding the susceptibility profile of the enterobacteria, the highest resistance rates were to tetracycline (38.8%) and amoxicillin-clavulanic acid (26.5%). Among P. aeruginosa, the highest resistance was observed for cefoxitin (100%). Regarding the production of the AmpC enzyme, 30% of the microorganisms in the CESP (Citrobacter spp., Enterobacter spp., Serratia spp. and Providencia spp.) group, and 100% of the P. aeruginosa were resistant to cefoxitin. The remaining microorganisms of the CESP group (70%) that were sensitive to cefoxitin were subjected to a confirmatory test, and 37.5% were found to be positive for the production of the AmpC enzyme. Regarding metallo-beta-lactamase, 23.8% of the non-fermenting gram-negative bacteria showed reduced sensitivity to imipenem, meropenem or ceftazidime. When subjected to the confirmatory test, 8% of the P. aeruginosa were positive for the MBL enzyme. Regarding the clinical signs and symptoms of infection, the highlighted results with >70% frequency are: opaque and/or reddish brown discoloration; increase in exudate volume and pain. Stage-three infection was the most prevalent (71.4%). An association was found between cellulitis and friable granulation tissue that bleeds easily and the culture for GNB. In conclusion, the presence of gram-negative pathogens with resistance profiles in primary healthcare patients suggests the need to implement microbiological surveillance for patients with VU experiencing a prolonged or difficult healing process, and that the identification VU infection should be guided by knowledge regarding the etiology, classic characteristic and clinical stages of infection.Item Análise da ocorrência de polimorfismo de nucleotídeo único do gene DOCK9 em ceratocone(Universidade Federal de Goiás, 2016-02-23) Reis, Leonardo Mariano; Taleb, Alexandre Chater; http://lattes.cnpq.br/3191620401627150; Ávila, Marcos Pereira de; http://lattes.cnpq.br/3335187297522447; Ávila, Marcos Pereira de; Taleb, Alexandre Chater; Alves, Milton Ruiz; Rassi, Alan Ricardo; Silva, Leopoldo Magacho dos SantosKeratoconus is a non-inflammatory ocular dysfunction characterized by thinning, protrusion and conical shape of the cornea. The progression of this dysfunction leads to significant decrease in visual acuity and occasionally, in more severe cases, to corneal transplantation. The etiology of keratoconus is complex and the genetic component is among the main factors associated with the development of this disease. The objective of this study was to evaluate the occurrence of mutation in candidate genetic loci and its relation with keratoconus in patients attended in Brazil compared to healthy volunteers, through analysis of single nucleotide polymorphism in the gene DOCK9. In this clinical study, 108 participants were evaluated: 46 keratoconus patients and 62 healthy volunteers (controls). DNA samples were extracted from collected blood from keratoconus patients and controls. The genotyping of the single nucleotide polymorphism rs7995432 in the gene DOCK9 was determined through realtime polymerase chain reaction (qPCR). Single nucleotide polymorphism mutations were observed in both patients and controls. There were no significant differences on the frequency and discrimination of the mutant and wild alleles between patients and controls. The frequency of the mutant allele (C) was 4.8% in patients and 7.6% in controls. For the wild allele (T), the frequencies were 95.2% in patients and 92.4% in controls. The heterozygous genotype was present in 9.5% of patients and 11% of controls, while the homozygous genotype for the wild allele (TT) was found in 90.5% and 87% for patients and controls, respectively. Thus, these results confirm no association of these mutations and the occurrence of keratoconus for this population.Item Análise dimensional da aresta lateral de corte e núcleo de instrumentos reciprocantes antes e após o preparo do canal radicular(Universidade Federal de Goiás, 2016-05-02) Sampaio , Felipe Cavalcanti; Decurcio , Daniel de Almeida; http://lattes.cnpq.br/4640062828556657; Estrela , Carlos; http://lattes.cnpq.br/3611967334176683; Estrela , Carlos; Alencar , Ana Helena Gonçalves de; Decurcio , Daniel de Almeida; Veloso , Heloísa Helena Pinho; Pécora , Jesus DjalmaObjective: The purpose of the present study was to analyze the influence of flute and shank dimensions present at the first 4mm to occurrence of plastic deformations and dimensional alterations in reciprocating instruments after RCP. Methodology: The reciprocating instruments used were Reciproc® R25, R40 and R50, WaveOne® Small, Primary and Large, Unicone® #20, #25 and #40. Scanning electron microscopy images were obtained of the first 4mm from the instrument’s tip (30X magnification) before and after shaping of simulated curved root canals. The instruments were used only once. The images were transferred to software AxioVision® to measure the instruments: flute area (µm2), shank area (µm2), flute longitudinal length (µm) and instrument transversal diameter (µm). The difference of data before and after root canal preparation (RCP) was compared by Students’ T test for paired samples and differences between instruments of similar sizes was performed by ANOVA followed by Tukey’s test. The instruments were classified for the plastic deformations presence after RCP. Results: Reciproc® instruments showed larger flutes and smaller shanks. The Reciproc® R40 showed significant difference for transversal diameter at 0.5mm from the tip. Reciproc® had no plastic deformations. Unicone® instruments showed significant differences on #20 instruments for transversal diameter at 1.5 and 3.0mm from the tip, and instrument #25 had difference at 1.5 and 3.0mm and second and third flute longitudinal length. Plastic deformations were visualized on one instrument #20m and on three #40. WaveOne® instruments showed significant differences for first and fourth flutes length of WaveOne® Primary, and tranversal diameter at 2.0mm from the tip of WaveOne® Large. Plastic deformations were present in two of three Large instruments. Conclusions: In summary, Reciproc® instruments had greater area and length of flutes and smaller shanks compared to Unicone® and WaveOne® of similar sizes. Reciproc® showed greater flute to shank ratio. WaveOne® had the lowest flute to shank ratio. Unicone® instruments showed more plastic deformations. Higher flutes and lesser shanks had less plastic deformations after curved RCP.Item Análise do desempenho da revisão rápida de 100% na detecção de resultados falso-negativos dos exames citopatológicos cervicais(Universidade Federal de Goiás, 2009-06-30) MANRIQUE, Edna Joana Cláudio; AMARAL, Rita Goreti; http://lattes.cnpq.br/3665611660713029Objectives: analyze the performance of the 100% rapid re-screening in detecting falsenegative results of cervical screening cervical, in quality control, after routine screening, using the average time of one and two minutes, according to final diagnosis. Methodology: a total 5,235 smears, classified as negative and unsatisfactory by routine screening, were submitted to 100% rapid re-screening method, using the time average of one and two minutes. In these reviews, the smears classified as unsatisfactory or suspects were subjected to detailed review. The concordant results were considered final diagnosis; the differences were meeting for a consensus that defined the final diagnosis. Results: of 5,235 smears submitted rapid re-screening method, of using the time of one minute and two minutes there was sensitivity and specificity of the final method of 64.3% and 99.2% for the time of one minute and two minutes was 63.8% and 99.5%. In smears, with satisfy adequacy for analysis, the sensitivity and specificity of this method, using the time of one and two minutes, were 64.2%, 98.9%, 61.5% and 99.4% respectively. The smears, with the adequacy of the smears presented for analysis limits, the sensitivity and specificity, using the time of one minute, was 64.7%, 99.9% and for two minutes were 70.6% and 99.8%. Of the total of 5,121 cervical smears, had 958 (18.7%) clinical information, after being submitted to rapid rescreening, using the time of one minute, 18 of those were suspects, of which ten were confirmed by final diagnosis as abnormal. When submitted to rapid re-screening using the time of two minutes, 13 were suspects, nine of these were confirmed by final diagnosis as abnormal. A total 4,163 (81.3%) smears had no clinical information, after being submitted to rapid re-screening, using the time of one minute were 70 suspects, of which 35 were classified as abnormal. When submitted to rapid re-screening using the time of two minutes were 54 suspects, of which 35 were confirmed by final diagnosis as abnormal. A rapid re-screening showed a sensitivity to smear with clinical information, using the time of one minute of 83.3% and for two minutes of 75%. Conclusions: the rapid re-screening method of 100% showed no difference in the detection of falsenegative results using the time of a minute or two. The adequacy of the sample does not influence the detection of false-negative results, using both a time as two minutes, and there was no difference in the detection of false-negative smears with and without clinical information using a time-two minutes and finally, in smears with clinical informationItem Análise do um programa desenvolvimento de enfermeiros gerentes em um hospital público na perspectiva da pesquisa ação(Universidade Federal de Goiás, 2010-06-18) MOTTA, Kátya Alexandrina Matos Barreto; MUNARI, Denize Bouttelet; http://lattes.cnpq.br/8409035360598716This study was made based on the theory of two main central axes. The first one explores the work of the head nurse in the context of a hospital. The second one explores the fundaments of the action research in the perspective of Kurt Lewin, showing the aspects that support the group intervention, as well as the conduction of the research. A descriptive research, with a qualitative approach, based on the action research, developed from an action in which researchers and participants got involved in a cooperative and participative way. The study was made between August/2007 and June/2008 in Goiânia/GO, there were 24 head nurses participants. The results of this work were consolidated in four articles that explored: 1. The head nurses capacitating process mediated by the action research; 2. The critical points of the nurses-administrators in the public hospital; 3. Analysis of the managing development of nurses according to Bion, and 4. Indicatives of behavior competences of the nurse manager in a public hospital. The results demonstrate a scenario with adverse critical points which come up in the work environment of the head nurse when performing his/her duties. The head nurses were capable of elaborating a diagnosis about themselves, mapping out their strong points, and the points to be developed as well, at the same time that they revealed a lot of difficulty to manage their units such as pressure, work overload, lack of technological and material resources, man power, lack of autonomy and time to plan the actions, excess work, among other things. Because of that we realize that the action research is an efficient tool as it mediated the theoretical and living learning about the challenges it is to manage the units which the nurses are responsible.Item Análise dos fatores epidemiológicos, clínico-patológicos e expressão das proteínas OCT4 e NANOG em amostras de melanoma cutâneo(Universidade Federal de Goiás, 2016-12-13) Silva, Constanza Thaise Xavier; Saddi, Vera Aparecida; http://lattes.cnpq.br/7496804650895441; Guillo, Lidia Andreu; http://lattes.cnpq.br/3401436781775091; Guillo, Lidia Andreu; http://lattes.cnpq.br/3401436781775091; Saddi, Vera Aparecida; http://lattes.cnpq.br/7496804650895441; Costa, Cesar Augusto Sam Tiago Vila Nova; Carneiro, Lilian Carla; Arruda, Jalsi TaconCutaneous melanoma is a type of skin cancer that originates in melanocytes, predominantly affecting young and middle-aged adults. Several evidence suggests that cancer stem cells exist for melanoma. The POU5F1 / OCT4, NANOG genes have been studied as cancer stem markers. OCT4 and NANOG are involved in the maintenance of pluripotency and self-renewal of undifferentiated embryonic stem cells. This study aims to evaluate the epidemiological, clinical-pathological and expression of the OCT4 and NANOG proteins in cutaneous melanoma samples. We selected 102 cases for the epidemiological, clinical and pathological study, diagnosed between the years 2004 to 2008. For survival study, patients with a followup of up to 60 months were selected, with a recorded death. Of the 102 cases evaluated, 62.7% were female and 37.3% male; With mean age of 57.2 years and 63.1 years respectively (p= 0.0026). The most prevalent age at diagnosis of melanoma was between 51 and 70 years (44.1% p= 0.023). In this study, there was a predominance of lesions located in the trunk (32.3%). Histopathological examination of the type of tumor growth showed a predominance of the superficial extensive type in 52.9% of the cases. According to the Breslow index, lesions with ≤1.0 mm predominated in 39.2% of the individuals, followed by lesions> 4.0 mm in 23.5% of the cases. According to the Clark level 29.4% of the cases were classified in level IV; Followed by 25.5% cases with level V; Clark II in 23.5%; Clark III in 20.6%; And Clark I in only 1 case (1.0%). There were metastases in 47% of the cases and the main localization sites were: lymph nodes, brain, skin and lung. Regarding the clinical evolution of the patients, there were 26 cases of deaths due to melanoma (25.5%). The survival curve calculated at the 60-month follow-up was 73.0%. Univariate analysis revealed significant associations between nuclear overexpression of OCT4 and the following variables: Breslow index with thickness > 2.1 mm (p = 0.021, OR: 2.64, 95% CI: 1.15-6.05 ); Levels of Clark, IV and V (p = 0.001, OR: 4.11, 95% CI: 1.79-9.46); ulceration present (p≤0.0001; OR: 459.0; 95% CI: 51.67-4077.27); Presence of metastases (p <0.0001, OR: 40.25, 95% CI: 12.90- 125.62), and death from cutaneous melanoma (p <0.0001). The significant associations between cytoplasmic hyperexpression of OCT4 and the following variables were: present ulceration (p = 0.015, OR: 2.73, 95% CI: 1.21-6.16); Presence of metastases (p = 0.004, OR: 3.34, 95% CI: 1.47 - 7.62) and death from cutaneous melanoma (p = 0.039, OR 2.67, 95% CI 1.05 - 6,77). And the significant associations between the cytoplasmic hyperexpression of NANOG and the following variables were: present ulceration (p≤ 0.0001); Presence of metastases (p ≤ 0.0001) and death due to cutaneous melanoma (p = 0.030). Our study demonstrated a strong association of the OCT4Item Análise endotelial da córnea em pacientes submetidos à cirurgia corneal pela técnica de anel intraestromal(Universidade Federal de Goiás, 2012-06-12) SILVA, Rodrigo Salustiano Corrêa e; PEREIRA, Ricardo Antonio; http://lattes.cnpq.br/6293178284668220; ÁVILA, Marcos Pereira de; http://lattes.cnpq.br/3335187297522447To evaluate corneal endothelium by means of specular microscopy exam in patients with keratoconus, before and after Cornealring® corneal intrastromal ring surgery. A hundred and two eyes of sixty-seven patients, aged between 12 and 45, with the average age of 27,31 ± 8,15 years, 30 of whom females and 37 males, were selected to be submitted to the implant of Cornealring® corneal ring segments and evaluation of the corneal endothelium in the pre and post surgery (six months after the procedure) in the External Diseases and Cornea Ward of the Instituto Panamericano da Visão. Data have been stored for later analysis by software SPSS (Statistical Package of Social Science) for Windows 15.0. Only the eyes receiving two rings with 250 μ thickness showed significant statistical difference (p = 0,008). The remaining eyes showed no statistical difference (initial x final). In relation to the thickness and to the number of implanted rings, a decrease in the final coefficient was observed in most implanted rings. However, only the eyes that received two rings 250 μ thick showed significant difference (p=0,003), with a decrease of 10,1% in the coefficients of variation average. In relation to the thickness and number of implanted rings, a decrease in the percentage of hexagonal cells was observed (before and after surgery). Only the eyes that received two rings 250 μ thick showed a highly significant decrease (p=0,001) in relation to the hexagonal percentage of 9,7%. Although the parameters in the specular microscopy showed alterations mainly in the thicker rings, all the patients showed transparent corneas without clinical signs of endothelial suffering along the six months of follow-up. A longer period of study must be considered for better understanding and interpretation of the results obtained, in order to establish any relationship between eventual complications and the thickness of the implanted ring.Item Análise epidemiológica dos cânceres de pulmão e da laringe em 30 anos(Universidade Federal de Goiás, 2014-06-26) Martins, Edesio; Oliveira, José Carlos de; Curado, Maria Paula; http://lattes.cnpq.br/3397823736381748; Curado, Maria Paula; Campoli, Paulo Moacir de Oliveira; Rabahi, Marcelo Fouad; Freitas, Nilceana Maya Aires; Paula, Adriano Augusto Peclat deIntroduction: Studies have shown a relationship between smoking and various cancers, especially in the respiratory tract, causing major impact on public health. Lung cancer is the leading cause of cancer death in the world, accounting for 20 %, while Laryngeal cancer represents 3 %. Objective: To analyze trends in incidence and mortality from lung and larynx cancer in Goiania in 20 years and to assess trends in mortality for these cancers, over the past 30 years for the Brazilian regions. Methods: All incident cases of lung cancer and larynx were obtained from the database registered in Goiania RCBP/ACCG and deaths were extracted from the Mortality Information System (SIM/MS) for both, Goiania as for other Brazilian regions. Incidence rates and standardized mortality rates were calculated using the world standard population and calculating the trend used the Poisson regression model using Joinpoint Regression Program, Version 4.0.4. Graphs and tables were made using the Excel 2013 software. Results: Two points were analyzed: the first showed that the trend of incidence for lung and larynx cancers in both sexes and age groups in Goiania, were in contrast to trend mortality for lung cancer in women over 50 increased by 2.5% per year, during the study period in Goiania. The second article assessed the trends in national mortality for cancers of the lung and larynx showing that in Brazil, the mortality rates for lung cancer ranged from 14.30 to 15.52/100,000 in men and 3.99 to 7.37/100,000 in women from 1980 to 2009. Laryngeal cancer in this variation was from 3.76 to 3.59/100,000 for males and 0.47 to 0.38/100,000 in the female. The analysis of trends showed increased mortality for lung cancer in both male and female genders respectively (APC 1.7 %; 95% CI 1.6 to 1.8, p < 0.001, and APC 4.1%; 95% CI 3.9, the 4.2, p < 0.000). Mortality rates for laryngeal cancer in men the increase was 1.4 % (95% CI 1.2 to 1.5 p < 0.0001), the rate for women increased by 1.3 % (95 % 0.9 -1.6, p < 0.0001) during the study period, so there were similar for both neoplasms. Conclusion: Incidence trends were stable for lung and larynx cancer in both sexes and age groups, in contrast, for women aged over 50 years there has been increasing trends in mortality in Goiania. When Abstract xviii analyzing mortality at the national level, there was decline for lung cancer in south and southeast whereas for cancer of the larynx, there was a decrease only in southeastern Brazil.Item Análise molecular do polimorfismo do gene da enzima óxido nítrico sintase endotelial na endometriose(Universidade Federal de Goiás, 2017-03-14) Silva, Rita de Cássia Pereira da Costa e; Moura, Kátia Karina Verolli de Oliveira; http://lattes.cnpq.br/0087299570422353; Guillo, Lídia Andreu; http://lattes.cnpq.br/3401436781775091; Guillo, Lídia Andreu; Reis, Paulo Roberto de Melo; Alves, Maria; Chen, Lee Chen; Ramos, Débora Pereira SantanaEndometriosis is defined by the emergence of foci of endometrial tissue with glandular and stromal or characteristics identical to those of the uterine cavity in other locations than the endometrium and focuses primarily on women of reproductive age. It is present in 10% of the general population and in 50% of cases of infertility, showing a strong genetic component by correlating the disease with various polymorphisms. One of them, G894T of eNOS gene of the enzyme endothelial nitric oxide synthase (eNOS) has been studied by have implications with infertility. The present study examined the allele frequency and genotype of this polymorphism in women with endometriosis storied in fertile and infertile. Blood samples were used 47 women with endometriosis from a center of reference in laparoscopy and infertility of the city of Goiânia, Goiás. The DNA was extracted and measured for genotypic analysis (PCR/RFLP) with primers and controls to enlarge the gene eNOS G894T. In the statistical analysis we used the Chi square tests and/or Fisher and Mann-Whitney. The patients had an average age 32.5 ± 2.9 years. Was demonstrated difference in average age between fertile and infertile patients p = 0.034, fertile was demonstrated 39,9± 3,0 anos, and infertile 37,3 ± 5,6 anos. Genotype polymorphism frequencies in study were: GG 59.6% 29.8% and GT TT 10.6%; to split into sub-groups of fertile and infertile met respectively: 70% and 50% GG; GT 30% and 29.2%; TT was null between fertile and infertile 20.8%. The allele frequency between the fertile and infertile, was, respectively: G 85% and 15% T; G 64.6% and 35.4% T, p = 0.04 (95% CI 3.0 OR = 1.12-8.29). In the frequency analysis of genotypic heritage models, codominant and recessive were respectively: (GG vs. TT) 59.6% (28/47) vs. 10.6% (5/47) p = 0.044 and recessive (TT vs. GT + GG) was 10.6% (5/47) vs. 89.4% (42%) p = 0.049. The study showed that the presence of the T allele of eNOS gene G894T polymorphism of endothelial nitric oxide synthase enzyme in fertile and infertile patients have an important role in the increase of endometriosis and in homozygous T allele confers risk for the infertility in endometriosis in women in the city of Goiânia-Goiás.Item Análise multidimensional do preparo de canais radiculares curvos com instrumentos de níquel-titânio em rotação contínua e reciprocante(Universidade Federal de Goiás, 2016-11-18) Mamede Neto, Iussif; Borges, Álvaro Henrique; http://lattes.cnpq.br/5540410063427054; Estrela, Carlos; http://lattes.cnpq.br/3611967334176683; Estrela, Carlos; Alencar, Ana Helena Gonçalves de; Decurcio , Daniel de Almeida; Borges, Álvaro Henrique; Pécora, Jesus DjalmaObjective: To evaluate transportation (T) and centering ability (CA) of root canal preparations using continuous or reciprocating nickel-titanium endodontic files. Material and methods: Ninety-six mesiobuccal root canals of mandibular first and second molars were randomly divide into 6 groups (n=16) according to the rotary file used: Group 1- ProTaper Next®; Group 2- ProTaper Gold®; Group 3- Mtwo®; Group 4- BioRaCe®; Group 5- WaveOne Gold®; Group 6- Reciproc®. Root canals were prepared according to manufacturer’s instructions. Cone-beam computed tomography scans were obtained before and after canal preparation. Measurements were made at five different points: 2, 3 and 4 mm from the apex and 2, 3 and 4 mm below furcation. Transportation and centering ability were analyzed following the recommendations made by Gambill et al. (1996). Results: The greatest mesiodistal (MD) transportation (T) was found for Reciproc® files (p<0.05), and the greatest buccolingual (BL) T, for Reciproc®, ProTaper Gold® and ProTaper Next® files (p<0.05). The greatest mesiodistal (MD) centering ability (CA) was found for BioRaCe® files (p<0.05), and the greatest buccolingual (BL) CA, for BioRaCe® and Mtwo® files (p<0.05). Conclusion: All systems produced root canal transportation. No file system achieved perfect CA of root preparation. Reciproc® files had the greatest MD T and BL T. BioRaCe® files had the greatest MD CA, whereas BL CA was similar for BioRaCe® and Mtwo® files.