REGIÃO METROPOLITANA DE GOIÂNIA (RMG)
URI Permanente desta comunidade
A Região Metropolitana de Goiânia compreende: Campus Colemar Natal e Silva, Campus Samambaia, Campus Aparecida de Goiânia os ministrados nesses campus são:
1) - Colemar Natal e Silva: Direito, Enfermagem, Engenharia Ambiental e Sanitária, Engenharia Civil e Ambietal,Engenharia da Computação, Engenharia Elétrica, Engenharia Mecânica, Farmácia,Medicina, Nutrição, Odontologia, Pedagogia e Psicologia.
2) - Campus Samanbaia: Administração, Agronomia, Arquitetura e Urbanismo, Artes Cênicas, Artes Plásticas, Artes Visuais, Biblioteconomia, Biomedicina, Biotecnologia, Ciências Biológicas,Ciências Contábeis, Ciências da Computação, Ciências Econômicas, Ciências Geoambientais, Ciências Sociais, Dança, Design de Ambientes, Design de Moda,Design Gráfico, Direção de Arte, Ecologia e Análise Ambiental, Educação Física, Engenharia de Alimentos, Engenharia de Software, Engenharia Florestal, Engenharia Química, Estatística, Filosofia, Física, Física Médica, Geografia, Gestão da Informação, História, Jornalismo, Letras, Licenciatura Intercultural, Matemática, Medicina Veterinária,Museologia, Música, Musicoterapia, Políticas Públicas, Publicidade e Propaganda, Química,Química Industrial, Relações Internacionais, Relações Públicas, Sistemas de Informação e
Zootecnia.
3) Campus Aparecida de Goiânia: Engenharia de Produção, Engenharia de Materais, Engenharia de Transportes e Geologia.
Navegar
Navegando REGIÃO METROPOLITANA DE GOIÂNIA (RMG) por Título
Agora exibindo 1 - 20 de 15792
Resultados por página
Opções de Ordenação
Item 1-(4-Methoxyphenyl)-2-(6-methyl-2-nitro- 3-pyridyloxy)propan-1-one(2005-01-08) Vencato, Ivo; Ferri, Pedro Henrique; Santos, Suzana da Costa; Pereira, Maristela; Lariucci, Carlito; Homar, Leon Ítalo Brasil; Napolitano, Hamilton BarbosaThe title compound, C16H16N2O5, is a -ketoether derivative closely related to natural 8,40-oxyneolignans, which are of interest because of their moderate antifungal activity against systemic mycosis. The nitro group is not coplanar with the aromatic ring, as shown by a torsion angle of 47.2 (4) . The molecules are linked by two non-classical intermolecular CÐ H O hydrogen bonds with distances between donors and acceptors of 3.441 (5) and 3.539 (5) A Ê , leading to the formation of molecular stacking perpendicular to the bc plane.Item 1-Methyl-7-(4-nitrophenyl)-3-phenyl- pyrazolo[3,4-b]pyrrolo[3,4-d]pyridine- 6,8(3H,7H)-dione(2008) Sabino, José Ricardo; Oliveira, Cecília Maria Alves de; Fraga, Carlos Alberto Manssour; Barreiro, Eliezer Jesus de Lacerda; Oliveira, Valéria de; Menegatti, RicardoItem A 12-year retrospective survey of management of patients with malignant neoplasms in the orbital cavity in a brazilian cancer hospital(2013) Sirianni, Daniela; Leles, Cláudio Rodrigues; Mendonca, Elismauro Francisco deAbstract: Purpose: The aim of this study was to investigate the distribution of different types of malignant tumors in the orbital cavity, surgical treatments, and subsequent prosthetic rehabilitation. Materials and Methods: A retrospective sample of 269 charts of patients attending a public reference cancer hospital in Goiania, Goias, Brazil, between 1998 and 2009 was selected. Study variables included demographic data, clinical and histopathological features, and patient rehabilitation. Frequency analysis, chi-square test and multiple logistic regression were used for data analysis. Results: Patients’ age ranged from 1 to 92 years and 50.2% were female. The most prevalent tumors were basal cell carci- noma (58%), retinoblastoma (17.1%), squamous cell carcinoma of the conjunctiva (7.1%), malignant melanoma (6.7%), and rhabdomyosarcoma (4.5%). Surgical treatment was performed in 250 patients (92.3%), but only 29 patients (10.8%) received ocular prosthetic rehabilitation. Lower age (p<0.001) and enucleation surgery (p<0.001) were significantly asso- ciated with prosthetic rehabilitation. Conclusions: Results revealed high occurrence of mutilating interventions and low provision of prosthetic care, limited to younger patients who had undergone enucleation surgery. The high unmet demand for rehabilitation emphasizes the need to incorporate appropriate care for ocular and orbital deformities into the Brazilian public health system.Item 14, 15-epoxygeranylgeraniol and extracts isolated from Pterodon emarginatus Vog. fruits: antitumor activity on glioblastoma cells(2012-09) Hansen, Daiane; Nakahata, Adriana Miti; Haraguchi, Mitsue; Okamoto, Oswaldo Keith; Gonçalez, Edlayne; Alonso, AntonioPlant-derived substances have traditionally played important roles in the treatment of human diseases, including of great significance to cancer therapy. Plants of the genus Pterodon (Fabaceae, Leguminosae), commonly known as ‘sucupira’, are disseminated throughout the central region of Brazil and have been used frequently in popular medicine. In recent years, interest in these plants has increased considerably. The biological effects of their extracts and pure metabolites have been investigated in several experimental models in vivo and in vitro. Until the present day, the antitumor effect of Pterodon plants on brain tumors is unknown. Therefore, the aim of this work was to investigate the action of P. emarginatus Vogel extracts and its fractions on glioblastoma cells. The hexane (HE), dichloromethane (DE) and ethanol (EE) extracts were obtained from seeds powder in each solvent. The diterpene 14,15-epoxygeranylgeraniol was obtained from HE fractionation. For tumorigenic assays, the extracts and fractions were added to U87MG, a human glioblastoma cells line. The cell viability assay showed that the proliferation of U87MG was inhibited by both extracts and the 14,15- epoxygeranylgeraniol. Further trials in vivo will help to confirm these results, and may contribute to generate natural compounds for the treatment of this type of cancer.Item 15N NMR shifts of eumelanin building blocks in water: a combined quantum mechanics/statistical mechanics approach(2020) Oliveira, Leonardo Bruno Assis; Fonseca, Tertius Lima da; Cabral, Benedito Jose CostaTheoretical results for the magnetic shielding of protonated and unprotonated nitrogens of eumelanin building blocks including monomers, dimers, and tetramers in gas phase and water are presented. The magnetic property in water was determined by carrying out Monte Carlo statistical mechanics sampling combined with quantum mechanics calculations based on the gauge-including atomic orbitals approach. The results show that the environment polarization can have a marked effect on nitrogen magnetic shieldings, especially for the unprotonated nitrogens. Large contrasts of the oligomerization effect on magnetic shielding show a clear distinction between eumelanin building blocks in solution, which could be detected in nuclear magnetic resonance experiments. Calculations for a π-stacked structure defined by the dimer of a tetrameric building block indicate that unprotonated N atoms are significantly deshielded upon π stacking, whereas protonated N atoms are slightly shielded. The results stress the interest of NMR experiments for a better understanding of the eumelanin complex structure.Item 1930: entre a memória e a história(Dulce Oliveira Amarante dos Santos, 2003-12) Sandes, Noé FreireThis essay seeks to analyse two historiographic books on 1930. The first is Barbosa Lima Sobrinho 's The truth on the Revolution of october; 24'h 1930 and the other is Boris Fausto 's! 930: history and historiography. Both narratives create differents pattems of hlstorical explication, so they keep a rich dialogue between memory and history as ways of historical consciencc.Item 19th Brazilian meeting on analytical chemistry(2020-03) Coltro, Wendell Karlos Tomazelli; Rocha, Fábio Rodrigo Piovezani; Teixeira, Leonardo Sena Gomes; Sodré, Fernando Fabriz; Santelli , Ricardo Erthal; Kubota , Lauro Tatsuo; Nóbrega, Joaquim de Araújo; Ferreira, Sergio Luis CostaItem 1D magnetic interactions in Cu Oxovanadium Phosphates (VPO), magnetic susceptibility, DFT, and single-crystal EPR(2015) Venegas Yazigi, Diego; Spodine, Evgenia; Saldias, Marianela; Vega, Andrés; Paredes García, Verónica; Calvo, Rafael; Santana, Ricardo Costa deWe report the crystal face indexing and molecular spatial orientation, magnetic properties, electron paramagnetic resonance (EPR) spectra, and density functional theory (DFT) calculations of two previously reported oxovanadium phosphates functionalized with CuII complexes, namely, [Cu(bipy)(VO2)(PO4)]n (1) and [{Cu(phen)}2(VO2(H2O)2)(H2PO4)2 (PO4)]n (2), where bipy = 2,2′-bipyridine and phen = 1,10-phenanthroline, obtained by a new synthetic route allowing the growth of single crystals appropriate for the EPR measurements. Compounds 1 and 2 crystallize in the triclinic group P1̅ and in the orthorhombic Pccn group, respectively, containing dinuclear copper units connected by two −O–P–O– bridges in 1 and by a single −O–P–O– bridge in 2, further connected through −O–P–O–V–O– bridges. We emphasize in our work the structural aspects related to the chemical paths that determine the magnetic properties. Magnetic susceptibility data indicate bulk antiferromagnetism for both compounds, allowing to calculate J = −43.0 cm–1 (dCu–Cu = 5.07 Å; J defined as Hex(i,j) = −J Si·Sj), considering dinuclear units for 1, and J = −1.44 cm–1 (dCu–Cu = 3.47 Å) using the molecular field approximation for 2. The single-crystal EPR study allows evaluation of the g matrices, which provide a better understanding of the electronic structure. The absence of structure of the EPR spectra arising from the dinuclear character of the compounds allows estimation of weak additional exchange couplings |J′| > 0.3 cm–1 for 1 (dCu–Cu = 5.54 Å) and a smaller value of |J′| ≥ 0.15 cm–1 for 2 (dCu–Cu = 6.59 Å). DFT calculations allow evaluating two different exchange couplings for each compound, specifically, J = −36.60 cm–1 (dCu–Cu = 5.07 Å) and J′ = 0.20 cm–1 (dCu–Cu =5.54 Å) for 1 and J = −1.10 cm–1 (dCu–Cu =3.47 Å) and J′ = 0.01 cm–1 (dCu–Cu = 6.59 Å) for 2, this last value being in the range of the uncertainties of the calculations. Thus, these values are in good agreement with those provided by magnetic and single-crystal EPR measurements.Item 1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequence(2014-04) Oliveira, Aline Lima de; Martinelli, Bruno César Barbosa; Liao, Luciano Morais; Pereira, Flávia de Castro; Lacerda, Elisângela de Paula Silveira; Alcantara, Glaucia BrazHigh resolution magic angle spinning 1H nuclear magnetic resonance spectroscopy (HR-MAS NMR) is a useful technique for evaluation of intact cells and tissues. However, optimal NMR parameters are crucial in obtaining reliable results. To identify the key steps for the optimization of HR-MAS NMR parameters, we assessed different pulse sequences and NMR parameters using sarcoma 180 (S180) cells. A complete assignment of the metabolites of S180 is given to assist future studies.Item 1H HRMAS NMR spectroscopy and chemometrics for evaluation of metabolic changes in citrus sinensis caused by xanthomonas axonopodis pv. citri(2012-05) Silva, Lorena Mara Alexandre e; Alves Filho, Elenilson de Godoy; Choze, Rafael; Liao, Luciano Morais; Alcantara, Glaucia BrazXanthomonas axonopodis (Xac) bacterium causes one of the most feared and untreatable diseases in citriculture: citrus canker. To understand the response mechanisms of orange trees when attacked by Xac, leaves and fruits of Citrus sinensis were directly evaluated by HRMAS NMR (high resolution magic angle spinning nuclear magnetic resonance) spectroscopy. This technique allows the analysis of samples without laborious pre-treatments and also allows access to important information about chemical composition of samples. The orange tree leaves and fruit peels investigated in this study demonstrated the biochemical changes caused by Xac. Aided by chemometric analysis, the HRMAS NMR results show relevant changes in amino acids, carbohydrates, organic acids and terpenoids content.Item 2,5-diketopiperazines via intramolecular N-alkylation of Ugi adducts: a contribution to the synthesis, density functional theory study, X-ray characterization, and potential herbicide application(2022) Mendes, Lorena Lessa; Varejão, Jodieh Oliveira Santana; Sousa, José Antonio de; Carneiro, José Walkimar de Mesquita; Valdo, Ana Karoline Silva Mendanha; Martins, Felipe Terra; Ferreira, Bruno Wesley; Barreto, Robert Weingart; Silva, Toshik Iarley da; Kohlhoff, Markus; Pilau, Eduardo Jorge; Varejão, Eduardo Vinícius VieiraTo investigate the herbicidal potential of 2,5-diketopiperazines (2,5-DKPs), we applied a known protocol to produce a series of 2,5-DKPs through intramolecular N-alkylation of Ugi adducts. However, the method was not successful for the cyclization of adducts presenting aromatic rings with some substituents at the ortho position. Results from DFT calculations showed that the presence of voluminous groups at the ortho position of a benzene ring results in destabilization of the transition structure. Lower activation enthalpies for the SN2-type cyclization of Ugi adducts were obtained when bromine, instead of a chlorine anion, is the leaving group, indicating that the activation enthalpy for the cyclization step controls the formation of the 2,5-DKP. Some Ugi adducts and 2,5-DKPs formed crystals with suitable qualities for single-crystal X-ray diffraction data collection. Phytotoxic damage of some 2,5-DKPs on leaves of the weed Euphorbia heterophylla did not differ from those caused by the commercial herbicide diquat.Item 2-(4-iodo-2,5-dimetoxifenil)-N-[(2-metoxifenil)metil]etamina ou 25I-NBOMe: caracterização química de uma designer drug(2016) Santos, Pâmella Ferraz dos; Souza, Lindamara Maria de; Merlo, Bianca Bortolini; Costa, Helber Barcellos da; Tose, Lilian Valadares; Santos, Heloa; Costa, Gabriela Vanini; Machado, Leandro Fernandes; Ortiz, Rafael Scorsatto; Limberger, Renata Pereira; Vaz, Boniek Gontijo; Romão, WandersonDrug trafficking and the introduction of new drugs onto the illicit market are one of the main challenges of the forensic community. In this study, the chemical profile of a new designer drug, 2-(4-iodine-2,5- dimethoxyphenyl)-n-[(2-methoxyphenyl)methyl]etamine or 25I-NBOMe was explored using thin layer chromatography (TLC), ultraviolet-visible spectrophotometry (UV-Vis), attenuated total reflection with Fourier transform infrared spectroscopy(ATRFTIR), gas chromatography mass spectrometry (GC-MS) and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR MS). First, the TLC technique was effective for identifying spots related to 25C-, 25B- and 25I-NBOMe compounds, all with the same retention factor, Rf ≈ 0.50. No spot was detected for 2,5-dimethoxy-4-bromoamphetamine, 2,5-Dimethoxy-4-chloroamphetamine or lysergic acid diethylamide compounds. ATR-FTIR preserved the physical-chemical properties of the material, whereas GC-MS and ESI-MS showed better analytical selectivity. ESI(+)FT-ICR MS was used to identify the exact mass (m/z 428.1706 for the [M + H]+ ion), molecular formula (M = C18H22INO3), degree of unsaturation (DBE = 8) and the chemical structure (from collision induced dissociation, CID, experiments) of the 25I-NBOMe compound. Furthermore, the ATR-FTIR and CID results suggested the presence of isomers, where a second structure is proposed as an isomer of the 25I-NBOMe molecule.Item 2-(Pyridin-4yl)benzothiazole and Its benzimidazole-analogue: biophysical and in silico studies on their interaction with urease and in vitro anti-Helicobacter pylori activities(2022) Pereira, Camila de Paula; Lyra, Ana Carolina Fradique de; Oliveira, Breno Germano de Freitas; Nascimento, Igor José dos Santos; Silva Júnior, Edeildo Ferreira da; Aquino, Thiago Mendonça de; Sisto, Francesca; Figueiredo, Isis Martins; Martins, Felipe Terra; Modolo, Luzia Valentina; Santos, Josué Carinhanha Caldas; Fátima, Ângelo deIn this study, the interaction between benzothiazole (BTA, concentration of a drug required for 50% inhibition in vitro (IC50) = 0.77 mM) and benzimidazole (BIA, IC50 = 2.14 mM) with urease was quantitatively assessed, using UV-Vis, molecular fluorescence, and circular dichroism. The results showed that both compounds interact with urease by a static fluorescence quenching mechanism with a non-fluorescent complex formation. The main forces responsible for stabilizing the supramolecular complex between BTA and urease were hydrophobic while, for BIA, van der Waals interactions and hydrogen bonds were the main ones. Urease conformation changes due to the interaction process were analyzed by circular dichroism and synchronous fluorescence. Besides, a competitive assay with substrate and inhibitors was used to evaluate the preferential urease site of interaction with BTA and BIA. Our experimental and theoretical studies supported that both, BTA and BIA, are mixed-inhibitors of ureases with a slight preference to the active site of such enzymes. Finally, both BTA and BIA showed to possess anti-Helicobacter pylori (one reference strain and six clinical isolates) activity, presenting minimal inhibitory concentration (MIC) values ranging from 38-150 and 20-164 µM, respectively. The urease inhibitors omeprazole and hydroxyurea showed MIC values in the range of 46-185 µM and 1683-> 3366 µM, respectively.Item 20 anos após a Lei 10.639: as encruzilhadas da educação física escolar(Universidade Federal de Goiás, 2023-02-08) Cesar, Victor Cavalcanti; Lobo, Pitias Alves; Lobo, Pitias Alves; Zanotto, Luana; Ferreira, Juliana de OliveiraThis work was motivated by the 20 years´ anniversary of the law 10.639 and aims to analyze the it´s crossroads with School Physical Education. It was realized a theoretical study, product of a bibliographical survey of tesis, dissertations, articles and books that dialogues with this thematic, having as its founder method the historical-dialectical materialism and intersectionality to explain phenomena such as racism and coloniality. In this direction, we dialogued with Simas and Rufino (2018) to reflect about a pedagogy violently anti-racist It was concluded that Ancestrality presents itself as a path for the restitution of black people`s protagonism and the formation of an identity capable of resignify the education and to promote good trades in the market-world.. The social neurosis produced by structural racism complicates the appropriation of sciences based in a counter colonialism epistemology. To overcome this problem was defended the contradiction of the imposition. Synthetically, it is about the use of communication to produce brooding knowledges, critics, capables of moving an historic plastered system. Within that, Scholar PE becomes a potent path between limits and possibilities to the superation of its pedagogical praxis, subverting the racist mono rationalism and sending away the unic history.Item 20 anos do curso de letras/espanhol da UFG: um sonho do Prof. Fernando Plaza(Letícia de Souza Carrijo, 2019-06) Silva, Cleidimar Aparecida Mendonça eThis article pays tribute to a Chilean professor, Fernando Plaza Mallea, who idealized and initiated the Spanish Language Teaching Programme at UFG, currently celebrating 20 years since the graduation of the first group. He taught Latin and Spanish at the former ICHL and at what we know today as the College of Letters, at the Federal University of Goias. Professor Plaza saw the possibility of a Spanish Teaching Education Programme after the creation of Mercosul, the South Common Market, in 1991, a partnership on free trade and customs between Brazil, Argentina, Paraguay and Uruguay; and also due to the fact that Brazil is surrounded by Spanish-speaking countries in South America. When professor Plaza retired in 1996, he could see his dream, though partially, fulfilled. According to the UFG Department of Registration and Diplomas, from 1998 to 2018, the Spanish Language Teaching Programme contributed to the education of 268 teachers. Many of these alumni have so far applied for different teaching selections and have taught in different levels (primary and secondary schools, technology institutions, and colleges). After being able to also witness some of his former students working as professors at the UFG Spanish Language Teaching Programme, professor Plaza died in 2004.Item 25I-NBOH: a new potent serotonin 5-HT2A receptor agonist identified in blotter paper seizures in Brazil(2017-02-16) Arantes, Luciano Chaves; Ferrari Júnior, Ettore; Souza, Luciano Figueiredo de; Cardoso, Andriele Costa; Alcantara, Thaynara Lino Fernandes; Liao, Luciano Morais; Machado, Yuri; Lordeiro, Rogério Araújo; Coelho Neto, José; Andrade, Ana Flávia Belchior deA new potent serotonin 5-HT2A receptor agonist was identified in blotter papers by several state level forensic laboratories in Brazil. The 25I-NBOH is a labile molecule, which fragments into 2C-I when analyzed by routine seized material screening gas chromatography (GC) methods. GC–mass spectrometry (MS), liquid chromatography–quadrupole time-of-flight-MS, and Fourier transform infrared and nuclear magnetic resonance analyses were performed to complete molecular characterization. Individual doses range from 300 to 1000 lg. Despite its being a potent 5-HT2A receptor agonist, 25I-NBOH is neither registered in the United Nations Office on Drugs and Crime (UNODC) nor classified as a scheduled substance in most countries. Sweden and Brazil seem to be the only countries to control 25I-NBOH. To our knowledge, this is the first scientific report dealing with identification of 25I-NBOH in actual seizures.Item 2D and 3D mixed MII/CuII metal–organic frameworks (M = Ca and Sr) with N,N′-2,6-pyridinebis(oxamate) and oxalate: preparation and magneto-structural study(2018) Fernandes, Tamires Soares; Melo, Wanessa David Canedo; Kalinke, Lucas Hoffmann Greghi; Souza Filho, Renato Rabelo de; Valdo, Ana Karoline Silva Mendanha; Silva, Cameron Capeletti da; Martins, Felipe Terra; Amorós, Pedro; Lloret Pastor, Francesc; Julve Olcina, Miguel; Gomes, Danielle Cangussu de CastroThree heterobimetallic complexes of formula [Ca2Cu3(mpyba)2(2-apyma)(H2O)7]·8.3H2O (1), [Sr2Cu3(mpyba)2(2-apyma)(H2O)8]·11.6H2O (2) and [Sr4.5Cu4(mpyba)4(ox)(H2O)20]·8.5H2O (3) [H4mpyba = N,N′-2,6-pyridinebis(oxamic acid), 2-apyma = 2-(6-aminopyridinyl)oxamate and ox = oxalate] have been synthesized and structurally characterized. Complexes 1 and 2 are isostructural compounds, with tricopper(II) units having mpyba and its hydrolytic product (2-apyma) as ligands. They are interlinked through strontium(II) (1) and calcium(II) (2) ions to afford neutral two-dimensional networks. Two of the copper(II) ions are five-coordinate in distorted square pyramidal (Cu3) and trigonal bipyramidal (Cu1) surroundings, whereas the other (Cu2) is six-coordinate in an elongated octahedral environment. The main difference between their structures, apart from the number of water molecules, resides in the nature of the alkaline earth cation coordinated to the oxamate fragments, Sr2+ (1)/Ca2+ (2), which exhibit eight and seven coordination, respectively. The π–π interactions and an extensive network of hydrogen bonds in 1 and 2 lead to supramolecular 3D structures. The relatively small size of their cavities, in the micropore domain, hinders the inclusion of N2 but allows CO2 adsorption (0.45 and 0.52 mmol g−1 for 1 and 2, respectively). The structure of 3 is made up of [3,3] metallacyclophane-type motifs, having the formula [Cu2(mpyba)2(H2O)2]4−. These act as tetrakis(bidentate) ligands towards the strontium(II) ions (Sr1, Sr2 and Sr3), leading to a sheet-like polymer growing in the bc plane, which extends further along the crystallographic a axis by a bis(chelating) oxalate between the Sr1 atoms. The investigation of the magnetic properties of 1–3 in the temperature range 1.9–300 K shows the occurrence of an overall antiferromagnetic behaviour for 1 and 2 [J12 = J23 = −9.71(2) (1) and −10.81(5) cm−1 (2), with the Hamiltonian being defined as H = −J12S1·S2 − J23S2·S3 + gβH[S1 + S2 + S3], and a ferromagnetic coupling within the dicopper(II) metallacylophane unit of 3 [J = +1.86(1) cm−1 through the Hamiltonian H = −JS1·S2 + gβH(S1 + S2)]. Simple orbital symmetry considerations (1–3) and the spin polarization mechanism (3) account for the observed magnetic properties.Item 2D quantitative imaging of magnetic nanoparticles by an ac biosusceptometry based scanning approach and inverse problem(2021) Biasotti, Gabriel Gustavo de Albuquerque; Próspero, Andre Gonçalves; Alvarez, Marcelo Dante Tacconi; Liebl, Maik; Pinto, Leonardo Antonio; Soares, Guilherme Augusto; Bakuzis, Andris Figueiroa; Baffa Filho, Oswaldo; Wiekhorst, Frank; Miranda, José Ricardo de ArrudaThe use of magnetic nanoparticles (MNPs) in biomedical applications requires the quantitative knowledge of their quantitative distribution within the body. AC Biosusceptometry (ACB) is a biomagnetic technique recently employed to detect MNPs in vivo by measuring the MNPs response when exposed to an alternate magnetic field. The ACB technique presents some interesting characteristics: non-invasiveness, low operational cost, high portability, and no need for magnetic shielding. ACB conventional methods until now provided only qualitative information about the MNPs’ mapping in small animals. We present a theoretical model and experimentally demonstrate the feasibility of ACB reconstructing 2D quantitative images of MNPs’ distributions. We employed an ACB single-channel scanning approach, measuring at 361 sensor positions, to reconstruct MNPs’ spatial distributions. For this, we established a discrete forward problem and solved the ACB system’s inverse problem. Thus, we were able to determine the positions and quantities of MNPs in a field of view of 5 5 1 cm3 with good precision and accuracy. The results show the ACB system’s capabilities to reconstruct the quantitative spatial distribution of MNPs with a spatial resolution better than 1 cm, and a sensitivity of 1.17 mg of MNPs fixed in gypsum. These results show the system’s potential for biomedical application of MNPs in several studies, for example, electrochemical-functionalized MNPs for cancer cell targeting, quantitative sensing, and possibly in vivo imaging.Item 3-phenacylideneoxindoles as a new class of antifungal compounds against Paracoccidioides spp(2023-01-20) Silva, Lívia do Carmo; Santos Filho, Raimundo Francisco dos; Oliveira, Amanda Alves de; Martins, Felipe Terra; Cunha, Silvio do Desterro; Soares, Célia Maria de Almeida; Pereira, MaristelaAims: Considering the need to identify new compounds with antifungal action, the activity of five 3-phenacylideneoxindoles compounds was evaluated. Materials & methods: The compounds were synthesized, and their antifungal activity was elucidated through minimum inhibitory concentration tests and interaction assay with other antifungals. Potential targets of compounds were predicted in silico. Results: 3-phenacylideneoxindoles compounds inhibited fungal growth with minimum inhibitory concentration and minimum fungicidal concentration ranging from 3.05 to 12.26 μM. The compounds demonstrated high selectivity index and presented a synergistic effect with itraconazole. In silico prediction revealed the pentafunctional AROM polypeptide, enolase, superoxide dismutase, catalase and kinases as proteins targets of the compound 4a. Conclusion: The results demonstrate that 3-phenacylideneoxindoles is a potential new class of antifungal compounds for paracoccidioidomycosis treatment.Item 3D printed microfluidic mixer for real-time monitoring of organic reactions by direct infusion mass spectrometry(2022) Duarte, Lucas da Costa; Silva, Igor Pereira da; Maciel, Lanaia Ítala Louzeiro; Vaz, Boniek Gontijo; Coltro, Wendell Karlos Tomazelli3D printing is a technology that has revolutionized traditional rapid prototyping methods due to its ability to build microscale structures with customized geometries in a simple, fast, and low-cost way. In this sense, this article describes the development of a microfluidic mixing device to monitor chemical reactions by mass spectrometry (MS). Microfluidic mixers were designed containing 3D serpentine and Y-shaped microchannels, both with a pointed end for facilitating the spray formation. The devices were fabricated entirely by 3D printing with fusion deposition modeling (FDM) technology. As proof-of-concept, micromixers were evaluated through monitoring the Katritzky reaction by injecting simultaneously 2,4,6-triphenylpropyllium (TPP) and amino acid (glycine or alanine) solutions, each through a different reactor inlet. Reaction product was monitored online by MS at different flow rates. Mass spectra showed that the relative abundances of the products obtained with the device containing the 3D serpentine channel were three times greater than those obtained with the Y-channel device due to the turbulence generated by the barriers created inside microchannels. In addition, when compared to the conventional electrospray ionization mass spectrometry (ESI-MS) technique, the 3D serpentine mixer offered better performance measured in relation to the relative abundance values for the reaction products. These results as well as the instrumental simplicity indicate that 3D printed microfluidic mixer is a promising tool for monitoring organic reactions via MS.