REGIÃO METROPOLITANA DE GOIÂNIA (RMG)

URI Permanente desta comunidade

http://200.137.215.59//handle/ri/575

A Região Metropolitana de Goiânia compreende: Campus Colemar Natal e Silva, Campus Samambaia, Campus Aparecida de Goiânia os ministrados nesses campus são: 1) - Colemar Natal e Silva: Direito, Enfermagem, Engenharia Ambiental e Sanitária, Engenharia Civil e Ambietal,Engenharia da Computação, Engenharia Elétrica, Engenharia Mecânica, Farmácia,Medicina, Nutrição, Odontologia, Pedagogia e Psicologia. 2) - Campus Samanbaia: Administração, Agronomia, Arquitetura e Urbanismo, Artes Cênicas, Artes Plásticas, Artes Visuais, Biblioteconomia, Biomedicina, Biotecnologia, Ciências Biológicas,Ciências Contábeis, Ciências da Computação, Ciências Econômicas, Ciências Geoambientais, Ciências Sociais, Dança, Design de Ambientes, Design de Moda,Design Gráfico, Direção de Arte, Ecologia e Análise Ambiental, Educação Física, Engenharia de Alimentos, Engenharia de Software, Engenharia Florestal, Engenharia Química, Estatística, Filosofia, Física, Física Médica, Geografia, Gestão da Informação, História, Jornalismo, Letras, Licenciatura Intercultural, Matemática, Medicina Veterinária,Museologia, Música, Musicoterapia, Políticas Públicas, Publicidade e Propaganda, Química,Química Industrial, Relações Internacionais, Relações Públicas, Sistemas de Informação e Zootecnia. 3) Campus Aparecida de Goiânia: Engenharia de Produção, Engenharia de Materais, Engenharia de Transportes e Geologia.

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1-(4-Methoxyphenyl)-2-(6-methyl-2-nitro- 3-pyridyloxy)propan-1-one
(2005-01-08) Vencato, Ivo; Ferri, Pedro Henrique; Santos, Suzana da Costa; Pereira, Maristela; Lariucci, Carlito; Homar, Leon Ítalo Brasil; Napolitano, Hamilton Barbosa
The title compound, C16H16N2O5, is a -ketoether derivative closely related to natural 8,40-oxyneolignans, which are of interest because of their moderate antifungal activity against systemic mycosis. The nitro group is not coplanar with the aromatic ring, as shown by a torsion angle of 47.2 (4) . The molecules are linked by two non-classical intermolecular CÐ H O hydrogen bonds with distances between donors and acceptors of 3.441 (5) and 3.539 (5) A Ê , leading to the formation of molecular stacking perpendicular to the bc plane.
1-Methyl-7-(4-nitrophenyl)-3-phenyl- pyrazolo[3,4-b]pyrrolo[3,4-d]pyridine- 6,8(3H,7H)-dione
(2008) Sabino, José Ricardo; Oliveira, Cecília Maria Alves de; Fraga, Carlos Alberto Manssour; Barreiro, Eliezer Jesus de Lacerda; Oliveira, Valéria de; Menegatti, Ricardo
A 12-year retrospective survey of management of patients with malignant neoplasms in the orbital cavity in a brazilian cancer hospital
(2013) Sirianni, Daniela; Leles, Cláudio Rodrigues; Mendonca, Elismauro Francisco de
Abstract: Purpose: The aim of this study was to investigate the distribution of different types of malignant tumors in the orbital cavity, surgical treatments, and subsequent prosthetic rehabilitation. Materials and Methods: A retrospective sample of 269 charts of patients attending a public reference cancer hospital in Goiania, Goias, Brazil, between 1998 and 2009 was selected. Study variables included demographic data, clinical and histopathological features, and patient rehabilitation. Frequency analysis, chi-square test and multiple logistic regression were used for data analysis. Results: Patients’ age ranged from 1 to 92 years and 50.2% were female. The most prevalent tumors were basal cell carci- noma (58%), retinoblastoma (17.1%), squamous cell carcinoma of the conjunctiva (7.1%), malignant melanoma (6.7%), and rhabdomyosarcoma (4.5%). Surgical treatment was performed in 250 patients (92.3%), but only 29 patients (10.8%) received ocular prosthetic rehabilitation. Lower age (p<0.001) and enucleation surgery (p<0.001) were significantly asso- ciated with prosthetic rehabilitation. Conclusions: Results revealed high occurrence of mutilating interventions and low provision of prosthetic care, limited to younger patients who had undergone enucleation surgery. The high unmet demand for rehabilitation emphasizes the need to incorporate appropriate care for ocular and orbital deformities into the Brazilian public health system.
14, 15-epoxygeranylgeraniol and extracts isolated from Pterodon emarginatus Vog. fruits: antitumor activity on glioblastoma cells
(2012-09) Hansen, Daiane; Nakahata, Adriana Miti; Haraguchi, Mitsue; Okamoto, Oswaldo Keith; Gonçalez, Edlayne; Alonso, Antonio
Plant-derived substances have traditionally played important roles in the treatment of human diseases, including of great significance to cancer therapy. Plants of the genus Pterodon (Fabaceae, Leguminosae), commonly known as ‘sucupira’, are disseminated throughout the central region of Brazil and have been used frequently in popular medicine. In recent years, interest in these plants has increased considerably. The biological effects of their extracts and pure metabolites have been investigated in several experimental models in vivo and in vitro. Until the present day, the antitumor effect of Pterodon plants on brain tumors is unknown. Therefore, the aim of this work was to investigate the action of P. emarginatus Vogel extracts and its fractions on glioblastoma cells. The hexane (HE), dichloromethane (DE) and ethanol (EE) extracts were obtained from seeds powder in each solvent. The diterpene 14,15-epoxygeranylgeraniol was obtained from HE fractionation. For tumorigenic assays, the extracts and fractions were added to U87MG, a human glioblastoma cells line. The cell viability assay showed that the proliferation of U87MG was inhibited by both extracts and the 14,15- epoxygeranylgeraniol. Further trials in vivo will help to confirm these results, and may contribute to generate natural compounds for the treatment of this type of cancer.
15N NMR shifts of eumelanin building blocks in water: a combined quantum mechanics/statistical mechanics approach
(2020) Oliveira, Leonardo Bruno Assis; Fonseca, Tertius Lima da; Cabral, Benedito Jose Costa
Theoretical results for the magnetic shielding of protonated and unprotonated nitrogens of eumelanin building blocks including monomers, dimers, and tetramers in gas phase and water are presented. The magnetic property in water was determined by carrying out Monte Carlo statistical mechanics sampling combined with quantum mechanics calculations based on the gauge-including atomic orbitals approach. The results show that the environment polarization can have a marked effect on nitrogen magnetic shieldings, especially for the unprotonated nitrogens. Large contrasts of the oligomerization effect on magnetic shielding show a clear distinction between eumelanin building blocks in solution, which could be detected in nuclear magnetic resonance experiments. Calculations for a π-stacked structure defined by the dimer of a tetrameric building block indicate that unprotonated N atoms are significantly deshielded upon π stacking, whereas protonated N atoms are slightly shielded. The results stress the interest of NMR experiments for a better understanding of the eumelanin complex structure.
1930: entre a memória e a história
(Dulce Oliveira Amarante dos Santos, 2003-12) Sandes, Noé Freire
This essay seeks to analyse two historiographic books on 1930. The first is Barbosa Lima Sobrinho 's The truth on the Revolution of october; 24'h 1930 and the other is Boris Fausto 's! 930: history and historiography. Both narratives create differents pattems of hlstorical explication, so they keep a rich dialogue between memory and history as ways of historical consciencc.
1H HR-MAS NMR and S180 cells: metabolite assignment and evaluation of pulse sequence
(2014-04) Oliveira, Aline Lima de; Martinelli, Bruno César Barbosa; Liao, Luciano Morais; Pereira, Flávia de Castro; Lacerda, Elisângela de Paula Silveira; Alcantara, Glaucia Braz
High resolution magic angle spinning 1H nuclear magnetic resonance spectroscopy (HR-MAS NMR) is a useful technique for evaluation of intact cells and tissues. However, optimal NMR parameters are crucial in obtaining reliable results. To identify the key steps for the optimization of HR-MAS NMR parameters, we assessed different pulse sequences and NMR parameters using sarcoma 180 (S180) cells. A complete assignment of the metabolites of S180 is given to assist future studies.
1H HRMAS NMR spectroscopy and chemometrics for evaluation of metabolic changes in citrus sinensis caused by xanthomonas axonopodis pv. citri
(2012-05) Silva, Lorena Mara Alexandre e; Alves Filho, Elenilson de Godoy; Choze, Rafael; Liao, Luciano Morais; Alcantara, Glaucia Braz
Xanthomonas axonopodis (Xac) bacterium causes one of the most feared and untreatable diseases in citriculture: citrus canker. To understand the response mechanisms of orange trees when attacked by Xac, leaves and fruits of Citrus sinensis were directly evaluated by HRMAS NMR (high resolution magic angle spinning nuclear magnetic resonance) spectroscopy. This technique allows the analysis of samples without laborious pre-treatments and also allows access to important information about chemical composition of samples. The orange tree leaves and fruit peels investigated in this study demonstrated the biochemical changes caused by Xac. Aided by chemometric analysis, the HRMAS NMR results show relevant changes in amino acids, carbohydrates, organic acids and terpenoids content.
2-(4-iodo-2,5-dimetoxifenil)-N-[(2-metoxifenil)metil]etamina ou 25I-NBOMe: caracterização química de uma designer drug
(2016) Santos, Pâmella Ferraz dos; Souza, Lindamara Maria de; Merlo, Bianca Bortolini; Costa, Helber Barcellos da; Tose, Lilian Valadares; Santos, Heloa; Costa, Gabriela Vanini; Machado, Leandro Fernandes; Ortiz, Rafael Scorsatto; Limberger, Renata Pereira; Vaz, Boniek Gontijo; Romão, Wanderson
Drug trafficking and the introduction of new drugs onto the illicit market are one of the main challenges of the forensic community. In this study, the chemical profile of a new designer drug, 2-(4-iodine-2,5- dimethoxyphenyl)-n-[(2-methoxyphenyl)methyl]etamine or 25I-NBOMe was explored using thin layer chromatography (TLC), ultraviolet-visible spectrophotometry (UV-Vis), attenuated total reflection with Fourier transform infrared spectroscopy(ATRFTIR), gas chromatography mass spectrometry (GC-MS) and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI-FT-ICR MS). First, the TLC technique was effective for identifying spots related to 25C-, 25B- and 25I-NBOMe compounds, all with the same retention factor, Rf ≈ 0.50. No spot was detected for 2,5-dimethoxy-4-bromoamphetamine, 2,5-Dimethoxy-4-chloroamphetamine or lysergic acid diethylamide compounds. ATR-FTIR preserved the physical-chemical properties of the material, whereas GC-MS and ESI-MS showed better analytical selectivity. ESI(+)FT-ICR MS was used to identify the exact mass (m/z 428.1706 for the [M + H]+ ion), molecular formula (M = C18H22INO3), degree of unsaturation (DBE = 8) and the chemical structure (from collision induced dissociation, CID, experiments) of the 25I-NBOMe compound. Furthermore, the ATR-FTIR and CID results suggested the presence of isomers, where a second structure is proposed as an isomer of the 25I-NBOMe molecule.
20 anos após a Lei 10.639: as encruzilhadas da educação física escolar
(Universidade Federal de Goiás, 2023-02-08) Cesar, Victor Cavalcanti; Lobo, Pitias Alves; Lobo, Pitias Alves; Zanotto, Luana; Ferreira, Juliana de Oliveira
This work was motivated by the 20 years´ anniversary of the law 10.639 and aims to analyze the it´s crossroads with School Physical Education. It was realized a theoretical study, product of a bibliographical survey of tesis, dissertations, articles and books that dialogues with this thematic, having as its founder method the historical-dialectical materialism and intersectionality to explain phenomena such as racism and coloniality. In this direction, we dialogued with Simas and Rufino (2018) to reflect about a pedagogy violently anti-racist It was concluded that Ancestrality presents itself as a path for the restitution of black people`s protagonism and the formation of an identity capable of resignify the education and to promote good trades in the market-world.. The social neurosis produced by structural racism complicates the appropriation of sciences based in a counter colonialism epistemology. To overcome this problem was defended the contradiction of the imposition. Synthetically, it is about the use of communication to produce brooding knowledges, critics, capables of moving an historic plastered system. Within that, Scholar PE becomes a potent path between limits and possibilities to the superation of its pedagogical praxis, subverting the racist mono rationalism and sending away the unic history.
20 anos do curso de letras/espanhol da UFG: um sonho do Prof. Fernando Plaza
(Letícia de Souza Carrijo, 2019-06) Silva, Cleidimar Aparecida Mendonça e
This article pays tribute to a Chilean professor, Fernando Plaza Mallea, who idealized and initiated the Spanish Language Teaching Programme at UFG, currently celebrating 20 years since the graduation of the first group. He taught Latin and Spanish at the former ICHL and at what we know today as the College of Letters, at the Federal University of Goias. Professor Plaza saw the possibility of a Spanish Teaching Education Programme after the creation of Mercosul, the South Common Market, in 1991, a partnership on free trade and customs between Brazil, Argentina, Paraguay and Uruguay; and also due to the fact that Brazil is surrounded by Spanish-speaking countries in South America. When professor Plaza retired in 1996, he could see his dream, though partially, fulfilled. According to the UFG Department of Registration and Diplomas, from 1998 to 2018, the Spanish Language Teaching Programme contributed to the education of 268 teachers. Many of these alumni have so far applied for different teaching selections and have taught in different levels (primary and secondary schools, technology institutions, and colleges). After being able to also witness some of his former students working as professors at the UFG Spanish Language Teaching Programme, professor Plaza died in 2004.
2D quantitative imaging of magnetic nanoparticles by an ac biosusceptometry based scanning approach and inverse problem
(2021) Biasotti, Gabriel Gustavo de Albuquerque; Próspero, Andre Gonçalves; Alvarez, Marcelo Dante Tacconi; Liebl, Maik; Pinto, Leonardo Antonio; Soares, Guilherme Augusto; Bakuzis, Andris Figueiroa; Baffa Filho, Oswaldo; Wiekhorst, Frank; Miranda, José Ricardo de Arruda
The use of magnetic nanoparticles (MNPs) in biomedical applications requires the quantitative knowledge of their quantitative distribution within the body. AC Biosusceptometry (ACB) is a biomagnetic technique recently employed to detect MNPs in vivo by measuring the MNPs response when exposed to an alternate magnetic field. The ACB technique presents some interesting characteristics: non-invasiveness, low operational cost, high portability, and no need for magnetic shielding. ACB conventional methods until now provided only qualitative information about the MNPs’ mapping in small animals. We present a theoretical model and experimentally demonstrate the feasibility of ACB reconstructing 2D quantitative images of MNPs’ distributions. We employed an ACB single-channel scanning approach, measuring at 361 sensor positions, to reconstruct MNPs’ spatial distributions. For this, we established a discrete forward problem and solved the ACB system’s inverse problem. Thus, we were able to determine the positions and quantities of MNPs in a field of view of 5 5 1 cm3 with good precision and accuracy. The results show the ACB system’s capabilities to reconstruct the quantitative spatial distribution of MNPs with a spatial resolution better than 1 cm, and a sensitivity of 1.17 mg of MNPs fixed in gypsum. These results show the system’s potential for biomedical application of MNPs in several studies, for example, electrochemical-functionalized MNPs for cancer cell targeting, quantitative sensing, and possibly in vivo imaging.
3D printed microfluidic mixer for real-time monitoring of organic reactions by direct infusion mass spectrometry
(2022) Duarte, Lucas da Costa; Silva, Igor Pereira da; Maciel, Lanaia Ítala Louzeiro; Vaz, Boniek Gontijo; Coltro, Wendell Karlos Tomazelli
3D printing is a technology that has revolutionized traditional rapid prototyping methods due to its ability to build microscale structures with customized geometries in a simple, fast, and low-cost way. In this sense, this article describes the development of a microfluidic mixing device to monitor chemical reactions by mass spectrometry (MS). Microfluidic mixers were designed containing 3D serpentine and Y-shaped microchannels, both with a pointed end for facilitating the spray formation. The devices were fabricated entirely by 3D printing with fusion deposition modeling (FDM) technology. As proof-of-concept, micromixers were evaluated through monitoring the Katritzky reaction by injecting simultaneously 2,4,6-triphenylpropyllium (TPP) and amino acid (glycine or alanine) solutions, each through a different reactor inlet. Reaction product was monitored online by MS at different flow rates. Mass spectra showed that the relative abundances of the products obtained with the device containing the 3D serpentine channel were three times greater than those obtained with the Y-channel device due to the turbulence generated by the barriers created inside microchannels. In addition, when compared to the conventional electrospray ionization mass spectrometry (ESI-MS) technique, the 3D serpentine mixer offered better performance measured in relation to the relative abundance values for the reaction products. These results as well as the instrumental simplicity indicate that 3D printed microfluidic mixer is a promising tool for monitoring organic reactions via MS.
3D printing of microfluidic devices for paper-assisted direct spray ionization mass spectrometry
(2016) Duarte, Lucas da Costa; Carvalho, Thays Colletes de; Lobo Júnior, Eulício de Oliveira; Abdelnur, Patrícia Verardi; Vaz, Boniek Gontijo; Coltro, Wendell Karlos Tomazelli
This study describes the use of a 3D printer to fabricate microfluidic devices for direct spray ionization mass spectrometry (DS-MS) assisted by paper tips. The layout of the proposed devices was designed in a three-dimensional model through a computer-aided design system and printed by a fused deposition modeling method using a thermoplastic filament composed of acrylonitrile butadiene styrene deposited layer by layer. The smallest channel width was 400 μm to ensure 3D printing uniformity without any obstruction. For DS-MS studies, microfluidic channels consisted of a single channel connected to a sample reservoir (3 mm diameter). The printed channel was 3 cm long, 500 μm wide and 500 μm deep. Paper tips (0.5 cm long × ca. 0.5 mm wide) were manually cut and inserted into the extremity of the printed channel to facilitate the spray formation. The spray was promoted by the application of 4 kV at the sample reservoir containing 0.1% formic acid prepared in methanol. This organic medium selected for MS experiments has demonstrated great compatibility with the polymeric material employed to create microfluidic chips. 3D printed devices were kept at a distance of 3 mm from the MS entrance. Using glucose solution as the model, the formed spray by the proposed microfluidic device was extremely stable in comparison to traditional paper spray ionization devices for at least 10 min. The analytical feasibility of printed devices for DS-MS was successfully demonstrated by qualitative analysis of ballpoint pen inks, caffeine, xylose and lysozyme. The 3D printer has allowed the fabrication of printed devices at a very low cost ($0.05) within 20 min. Furthermore, 3D printed devices have exhibited significant repeatability and reproducibility, making their reuse possible. Based on the performance of the proposed devices, we believe they can be used in a broad range of bioanalytical applications.
3x4: fotografia de prisão contemporânea e as representações do corpo encarcerado em duas prisões femininas de Moçambique
(Alice Fátima Martins, 2015-06) Sousa, Camila Maissune de; Monteiro, Rosana Horio
In this article, we discuss the representations of the imprisoned body in contemporary photography, seeking to understand how such representations have dialogued with current prison systems and thus yielded a series of discussions about authority and photography ethics. In dialogue with nineteenthcentury prison visuality and the concept of photographic performativity, and taking as starting point the “3x4” (a photographic project that took place in two Mozambican female prisons), we explore the interpretive possibilities offered by photography regarding the incarcerated body.
4-Fluorobenzaldehyde limonene-based thiosemicarbazone induces apoptosis in PC-3 human prostate cancer cells
(2018) Rodrigues, Bruna dos Santos; Marcelino, Renato Ivan de Ávila; Miranda, Polyana Lopes Benfica; Pires, Ludmila Bringel; Oliveira, Cecilia Maria Alves de; Vandresen, Fábio; Silva, Cleuza Conceição da; Valadares, Marize Campos
Aims: This study evaluated parameters of toxicity and antiproliferative effects of (+)-N(1)-4-fluorobenzaldehyde-N(4)-{1-methyl-1-[(1R)-4-methylcyclohexene-3-il]-ethyl}-thiossemicarbazone (4-FTSC) in PC-3 adenocarcinoma prostate cells. Main methods: Cytotoxicity of 4-FTSC in PC-3 cells was evaluated using MTT assay. Morphology examination of PC-3 cells treated with 4-FTSC was also performed as well as the cell death mechanisms induced were investigated using flow cytometry. Parameters of toxicity of 4-FTSC was conducted by the investigation of its potential myelotoxicity and lymphotoxicity, hemolytic activity and acute oral toxicity profile. Key findings: 4-FTSC showed promising cytotoxic effects against PC-3 cells (IC50 = 18.46 μM). It also triggered apoptotic morphological changes, phosphatidylserine externalization and a significant increase of DNA fragmentation in PC-3 cells. Moreover, 4-FTSC did not show changes in the PC-3 cell cycle with levels of p21, p27, NFĸB and cyclin D1 similar to those found in both control and treated cells. 4-FTSC also promoted an increase of p53 levels associated with mitochondrial impairment through loss of ∆Ψm and ROS overproduction. 4-FTSC-induced cell death mechanism in PC-3 cells involved activation of caspase-3/-7 through apoptosis intrinsic pathway via caspase-9. Regarding toxicological profile, 4-FTSC showed in vitro lymphotoxicity, although with low cytotoxicity for bone marrow progenitors and no hemolytic potential. Moreover, it was classified as GHS category 5 (LD50 > 2000–5000 mg/Kg), suggesting it has low acute oral systemic toxicity. Significance: 4-FTSC seems to be a promising candidate to be used as a clinical tool in prostate cancer treatment. Further studies are required to better clarify its toxicopharmacological effects found in this compound.
A 44 acima do 44: a moda plus size em Goiânia
(Universidade Federal de Goiás, 2021-06-03) Vilela, Jane Kelle Marques; Anjos Neto, João Dantas dos; Anjos Neto, João Dantas dos; Santos, Flávia Martins dos; Oliveira, Mayllon Lyggon de Sousa
This work seeks to analyze plus size fashion in the city of Goiânia. This research presents a historical perspective of fashion and the body adapted to it, discusses the creation of networks and sharing groups and the theories of marketing and digital marketing and the role of Public Relations in the segment. The analysis comprises a quantitative survey aimed at the niche of plus size consumers, and two qualitative surveys with influencers and retailers in the environment where perceptions about the plus size fashion market are revealed.
4D-QSAR: perspectives in drug design
(2010) Andrade, Carolina Horta; Pasqualoto, Kerly Fernanda Mesquita; Ferreira, Elizabeth Igne; Hopfinger, Anton J.
Drug design is a process driven by innovation and technological breakthroughs involving a combination of advanced experimental and computational methods. A broad variety of medicinal chemistry approaches can be used for the identification of hits, generation of leads, as well as to accelerate the optimization of leads into drug candidates. The quantitative structure–activity relationship (QSAR) formalisms are among the most important strategies that can be applied for the successful design new molecules. This review provides a comprehensive review on the evolution and current status of 4D-QSAR, highlighting present challenges and new opportunities in drug design.
A1 noradrenergic neurons lesions reduce natriuresis and hypertensive responses to hypernatremia in rats
(2013-09) Silva, Elaine Fernanda da; Oliveira, André Henrique Freiria de; Custódio, Carlos Henrique Xavier; Ghedini, Paulo César; Bataus, Luiz Artur Mendes; Colombari, Eduardo; Castro, Carlos Henrique de; Colugnati, Diego Basile; Rosa, Daniel Alves; Cravo, Sergio Luiz Domingues; Pedrino, Gustavo Rodrigues
Noradrenergic neurons in the caudal ventrolateral medulla (CVLM; A1 group) contribute to cardiovascular regulation. The present study assessed whether specific lesions in the A1 group altered the cardiovascular responses that were evoked by hypertonic saline (HS) infusion in non-anesthetized rats. Male Wistar rats (280–340 g) received nanoinjections of antidopamine-b-hydroxylase-saporin (A1 lesion, 0.105 ng.nL 21 ) or free saporin (sham, 0.021 ng.nL 21 ) into their CVLMs. Two weeks later, the rats were anesthetized (2% halothane in O 2 ) and their femoral artery and vein were catheterized and led to exit subcutaneously between the scapulae. On the following day, the animals were submitted to HS infusion (3 M NaCl, 1.8 ml N kg 21 , b.wt., for longer than 1 min). In the sham-group (n = 8), HS induced a sustained pressor response (DMAP: 3563.6 and 1161.8 mmHg, for 10 and 90 min after HS infusion, respectively; P,0.05 vs. baseline). Ten min after HS infusion, the pressor responses of the anti-DbH-saporin-treated rats (n = 11)were significantly smaller(DMAP: 1861.4 mmHg; P,0.05 vs. baseline and vs. sham group), and at 90 min, their blood pressures reached baseline values (261.6 mmHg). Compared to the sham group, the natriuresis that was induced by HS was reduced in the lesioned group 60 min after the challenge (19665.5 mM vs. 26267.6 mM, respectively; P,0.05). In addition, A1-lesioned rats excreted only 47% of their sodium 90 min after HS infusion, while sham animals excreted 80% of their sodium. Immunohistochemical analysis confirmed a substantial destruction of the A1 cell group in the CVLM of rats that had been nanoinjected withanti-DbH- saporin. These results suggest that medullary noradrenergic A1 neurons are involved in the excitatory neural pathway that regulates hypertensive and natriuretic responses to acute changes in the composition of body fluid.
A2 noradrenergic lesions prevent renal sympathoinhibition induced by hypernatremia in rats
(2012-05) Pedrino, Gustavo Rodrigues; Oliveira, André Henrique Freiria de; Colombari, Débora Simões Almeida; Rosa, Daniel Alves; Cravo, Sergio Luiz Domingues
Renal vasodilation and sympathoinhibition are recognized responses induced by hypernatremia, but the central neural pathways underlying such responses are not yet entirely understood. Several findings suggest that A2 noradrenergic neurons, which are found in the nucleus of the solitary tract (NTS), play a role in the pathways that contribute to body fluid homeostasis and cardiovascular regulation. The purpose of this study was to determine the effects of selective lesions of A2 neurons on the renal vasodilation and sympathoinhibition induced by hypertonic saline (HS) infusion. Male Wistar rats (280– 350 g) received an injection into the NTS of anti-dopamine-beta-hydroxylase-saporin (A2 lesion; 6.3 ng in 60 nl; n = 6) or free saporin (sham; 1.3 ng in 60 nl; n = 7). Two weeks later, the rats were anesthetized (urethane 1.2 g?kg 21 b.wt., i.v.) and the blood pressure, renal blood flow (RBF), renal vascular conductance (RVC) and renal sympathetic nerve activity (RSNA) were recorded. In sham rats, the HS infusion (3 M NaCl, 1.8 ml?kg 21 b.wt., i.v.) induced transient hypertension (peak at 10 min after HS; 962.7 mmHg) and increases in the RBF and RVC (14167.9% and 14067.9% of baseline at 60 min after HS, respectively). HS infusion also decreased the RSNA (24565.0% at 10 min after HS) throughout the experimental period. In the A2-lesioned rats, the HS infusion induced transient hypertension (661.4 mmHg at 10 min after HS), as well as increased RBF and RVC (13365.2% and 13466.9% of baseline at 60 min after HS, respectively). However, in these rats, the HS failed to reduce the RSNA (11563.1% at 10 min after HS). The extent of the catecholaminergic lesions was confirmed by immunocytochemistry. These results suggest that A2 noradrenergic neurons are components of the neural pathways regulating the composition of the extracellular fluid compartment and are selectively involved in hypernatremia-induced sympathoinhibition.

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