Doutorado em Ciência Animal (EVZ)
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Item Efeito da solução de Carolina Rinse na injúria de isquemia e reperfusão experimental no intestino delgado de coelhos(Universidade Federal de Goiás, 2011-11-18) BRANDSTETTER, Luciana Ramos Gaston; MOURA, Veridiana Maria Brianezi Dignani de; http://lattes.cnpq.br/8773201078957745; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; ARAÚJO, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928Mechanic obstruction of blood vessels that irrigate the intestine leads to ischemia and intense reduction of oxygenation and tissue perfusion. If oxygenated blood flow returns abruptly to tissues before cellular death reperfusion injury occurs; this can be initiated by several mechanisms resulting in inflammatory response. Intestinal ischemia/reperfusion (I/R) injury has been widely associated to cell necrosis, although another distinct biochemical and morphological type of cell death, called apoptosis, is involved. Such conditions are mediated by signaling molecules in cellular surface, which lead to long term changes in gene expression. In the majority of cells, MAP (Mitogen Activated Protein) kinases (MAPK), which are part of a group of cytoplasmic enzymes, will transmit mitogenic and cell differentiation signs. Three MAPK that have been identified in mammalian cells participate in the signaling pathway: ERK, p38 and JNK. Activated p38 and JNK are usually related to apoptosis, while activated ERK 1/2 (P44/42) have a protection function inhibiting apoptosis. With the purpose of minimizing reperfusion injury after liver transplant, Carolina Rinse Solution (CRS) was designed by North Carolina University, USA. Although this solution has been used in horses to attenuate I/R injury in horse intestine, little is known about the eventual tissue protection mechanism. The present study aimed to investigate the effects of topical and intraluminal CRS in I/R injury, and the activation profile of p38 and ERK 1/2 MAPK, in rabbit jejunum. Fifteen New Zealand rabbits were allocated to three groups: Sham-operated (A), Ischemia and reperfusion (B) and CRS (C). Groups B and C were subjected to one hour of jejunal ischemia and two hours of reperfusion. In group C, ten minutes prior to reperfusion, the bowel lumen was filled with CRS, and the segment was immersed in CRS until reperfusion onset. Changes such as degeneration, necrosis, edema, hemorrhage, PMN infiltration and margination were not significantly different between groups B and C, showing that topical and intraluminal CRS did not attenuate deleterious effects of I/R in small intestinal of rabbits. I/R stimulated the phosphorylation of p44/42 MAPK and p38 MAPK pathways in some layers of jejunum. Progressive activation of p44/42 MAPK was chiefly localized to the crypts of Lieberkühn, circular and longitudinal muscle layers, whereas p38 MAPK was prominently activated in myenteric plexus and both muscle layers. All layers that did respond to I/R insult with activation increase of ERK 1/2 and p38, in all groups, showed low baseline phosphorylation levels as compared to those that did not react to the insult. The results of this work indicate that topical and intraluminal CRS does not interfere in ERK 1/2 and p38 activation profile in rabbit jejunum subjected to I/R.Item Ação Neuroprotetora do Extrato Etanólico da Casca de Pequi em Cérebros de Ratos Submetidos à Isquemia de Reperfusão(Universidade Federal de Goiás, 2011-03-24) MIGUEL, Marina Pacheco; BRITO, Luiz Augusto Batista; http://lattes.cnpq.br/3212353728321020; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; ARAÚJO, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928Item Análise anatomo-funcional dos músculos do antebraço e a citoarquitetura do neocórtex occipital de Cebus libidinosus(Universidade Federal de Goiás, 2010-06-10) PRADO, Yandra Cássia Lobato do; FERREIRA, Tales Alexandre Aversi; http://lattes.cnpq.br/1101341437463729; DAMASCENO, Adilson Donizeti; http://lattes.cnpq.br/3900110295277130; ARAÚJO, Eugênio Gonçalves de; http://lattes.cnpq.br/3919777570059928Primates are known for larger brain size, enhanced handling and cognitive abilities and increasingly complex social behavior. Many studies have been conducted with the neotropical primates of the genus Cebus, known as capuchin monkey, regarding their behavior, tool use, encephalization index and memory. These studies are justified because these primates present high cognitive faculty and other biological characteristics that make them similar to Old World primates. This thesis rendered possible the production of two articles. The first one is entitled Anatomical description of the extensor muscles of the forearm associated with cognition and tool use in Cebus libidinosus . In this article, a comparative analysis between the anatomy of the forearm muscle of this genus and that the one of other primates that, individually or jointly, act in the hands allowing more or less specialized movements of the fingers, relating them to the use of tools and to the cognitive aspects of Cebus spp. described in the literature. There were evident similarities between the extensor muscles in C. libidinosus primates and the correspondent muscles in humans and chimpanzees; however, there were differences when compared to baboons. The structure and differentiation of the forearm muscles of Cebus spp., together with its previously known encephalization index, corroborate the association between its manual ability and cognitive and behavioral aspects in primates. The second article is entitled "Cytoarchitecture of the occipital neocortex of Cebus libidinosus . Its purpose was to analyze the cytoarchitecture of the occipital cortex using the technique of Golgi-Cox, the qualitative and quantitative aspects provided by this technique of impregnation of neurons, and to compare them with data from the literature. Primary and secondary visual areas are located in the occipital neocortex, and in C. libidinosus, the histological organization of these areas is similar to humans and other primates and it is characterized by the predominance of granular neurons and the presence of transversal fibers in the fourth layer. Although the Golgi-Cox method allowed individual observation of neurons and their extensions, the weak distinction between neurons and glial cells impaired neuron counting. These data, nevertheless, will grant further cell density comparison of other cortical areas between Cebus spp. and other primates. Aiming to contribute to the foundations of studies related to the evolution of primate cognition, this study related morphology, as a science, to the numerous observations on the behavior of capuchin monkeys. The comparative approach to study the morphology of the musculoskeletal and neural apparatus of C. libidinosus will allow data inference in areas of cognitive science and related areas.Item SISTEMA DE BARREIRA COM FILME DE HIDROGEL DE POLI(2-HIDROXIETIL METACRILATO) NA PREVENÇÃO DE ADERÊNCIAS PERITONIAIS : ESTUDO EXPERIMENTAL EM RATAS E CADELAS(Universidade Federal de Goiás, 2009-01-16) SILVA, Marcelo Seixo de Brito e; FIORAVANTI, Maria Clorinda Soares; http://lattes.cnpq.br/8772502020076257; PAULO, Neusa Margarida; http://lattes.cnpq.br/7949273598509043Synthetic materials are the leading choice for the correction of defects in abdominal wall, but the formation of adhesions are a problem encountered when placing implants in contact with the abdominal viscera. With In order to avoid its formation, various materials and mechanisms are used in an attempt to prevent contact with peritoneal surfaces of the prostheses. In this sense, we investigated the possibility of the hydrogel poly (2-hydroxyethyl methacrylate) (polyHEMA) coating a face of polypropylene mesh and thereby preventing peritoneal adhesion in dogs and rats, and, in the latter species, also performed a histological evaluation of implanted materials. For this, an abdominal defect was created in the rectus abdominis of 16 bitches were divided into two groups of eight animals and the defect was corrected with screen polypropylene and polypropylene mesh coated with hydrogel, respectively. The presence of adhesions was evaluated after 20 days by laparoscopy. As to rats, the surgical procedure was similar and the evaluations were performed at 15 and 30 days, with euthanasia and collection of fragments for histology with picrosirius red staining and HE The hydrogel coating the polyHEMA polypropylene mesh implanted in the abdominal wall prevented completely visceral adhesions and omentum remaining on the surface the screen when compared to polypropylene mesh in dogs and isolated rats. Was found greater amount of adhesions in the suture zone in the group a mesh coated with polyHEMA. The physical structure of the biomaterial interfered the organization and persistence of tissue response, but not in the same type.Item Células-tronco mesenquimais na terapia de lesões tendíneas iatrogênicas de coelhos: obtenção, aspectos histológicos e ultraestruturais(Universidade Federal de Goiás, 2012-02-17) SOUZA, Luiz Augusto de; LACERDA, Elisângela de Paula Silveira; http://lattes.cnpq.br/9390789693192751; BRITO, Wilia Marta Elsner Diederichsen de; http://lattes.cnpq.br/7605775995731168; SILVA, Luiz Antônio Franco da; http://lattes.cnpq.br/0446055905975647Most tendon injuries are related to trauma and involve partial or complete tendon section due to the action of cutting or sharp objects or laceration. Recently, medical advances have shown a growing interest in the use of cell therapy for the treatment of chronic and degenerative diseases with slow and ineffective healing. The aim of this study was to establish a protocol for mononuclear cells isolation from bone marrow (BM) of rabbits, followed by purification using negative depletion with the monoclonal antibody CD45 for separation of mesenchymal stem cells (MSCs), with further cultivation in MesenCult ® medium. After that, it was evaluated the effects of allogeneic transplantation of MSCs with or without collagen extracellular matrix (CEM) on wound healing of the common calcaneal tendon in rabbits after partial tenectomy. Clinical, physical, macroscopic, histological and electron microscopy evaluations of tendon fibers were conducted. It was concluded that the collection, isolation, purification and rapid expansion of MSCs obtained from the mononuclear fraction of BM can be achieved using the density gradient Ficoll-paque®, negative depletion of haematopoietic cells in immunomagnetic base and MesenCult® medium. Furthermore, it was noticed that the number of passages exerts a negative influence on the morphology of the cells. Regarding treatment of tendinous injuries, the group treated with MSCs from bone marrow cultured in MesenCult® soaked in collagen sponge showed better results in terms of progress and quality of healing with deposition of collagen fibers in longitudinal orientation.