Mestrado em Genética e Melhoramento de Plantas (EA)
URI Permanente para esta coleção
Navegar
Navegando Mestrado em Genética e Melhoramento de Plantas (EA) por Por Tipo de Defesa "Dissertação"
Agora exibindo 1 - 20 de 80
Resultados por página
Opções de Ordenação
Item Expressão de genes ortólogos relacionados à tolerância à seca em arroz(Universidade Federal de Goiás, 2014-04-04) Abreu, Fernanda Raquel Martins; Brondani, Claudio; http://lattes.cnpq.br/4775600104554147; Brondani, Claudio; Pereira, Maristela; Vianello, Rosana PereiraDrought, a major problem concerning a sustainable rice production in Brazil and worldwide, is responsible for a series of plant responses, including modification in gene expression, accumulation of metabolites and protein synthesis. In order to verify the correspondence between five Arabidopsis genes (PLDα1, LEW2, GluR2, Lsi1 e EIN2), previously related to drought tolerance, and their respective orthologs in rice, the present study analyzed two contrasting rice genotypes for drought, Douradão, the tolerant genotype, and Primavera, the susceptible one. The genotypes were submitted to drought stress and subsequently evaluated for gene expression by quantitative real-time Polymerase Chain Reaction (qPCR). The comparison of gene expression, between leaf and root tissues, showed a greater expression in roots, within their vegetative stage, and leaves, within their reproductive stage. Differential expression were observed mainly among the genes whose orthologs in Arabidopsis encode phospholipase Dα1 (PLDα1) and ethylene-insensitive protein (EIN2); these proteins are directly related to abscisic acid (ABA), a phytohormone that when identified in higher concentration in cells triggers the expression of drought stress-responsive genes, besides it is also responsible for the regulating the water loss (by transpiration) by controlling of stomatal movement. The results suggested that orthologs genes were in fact drought stress-responsive genes in rice, and emphasized the feasibility of PLDα1 and EIN2 overexpression in rice plants, supporting plant breeding programs in the development of drought tolerant genotypes.Item Variabilidade genética quantitativa e molecular em uma coleção de germoplasma de Eugenia dysenterica DC. (Myrtaceae)(Universidade Federal de Goiás, 2012-03-15) Almeida Júnior, Edivaldo Barbosa de; Chaves, Lázaro José; Soares, Thannya Nascimento; http://lattes.cnpq.br/5590256762396056; Soares, Thannya Nascimento; Chaves, Lázaro José; Vasconcellos, Breno de Faria e; Coelho, Alexandre Siqueira GuedesThe cagaiteira (Eugenia dysenterica DC)., is a native species of Cerrado. The plant is known for fruit production, which are used in natura or processed in several ways. It also provides food for the local fauna and, therefore, it conservation is important for maintenance for the communities. In order to maintain the productivity potential of the species, we should invest on plant breeding programs. To support these programs and help the species conservation, it is important to characterize the genetic variability available to breeders, both in germplasm collections and natural populations. This could also help to recommend priority areas to collect and conserve the germplasm. Neutral molecular markers have been used to evaluate the distribution of genetic variability in natural populations. The genetic structure of populations is the result of historical interaction between genetic drift, mutation, and gene flow. To detect the influence of adaptive processes in the genetic differentiation of populations we used 𝑄𝑆𝑇 index. The comparison of 𝑄𝑆𝑇 to the 𝐹𝑆𝑇, for neutral loci, provides values to test hypotheses about the role of natural selection. Therefore, the aim of this study was to characterize the germplasm collection of the cagaiteira from the EA/UFG. We used quantitative traits and microsatellite markers to make inferences about the role of natural selection in the differentiation of the cagaiteira subpopulations of Goiás, Southeast Brazil. Data collected from the quantitative traits were: plant height (AB), height of the first bifurcation (AB), the stem circumference (CC) and mean diameter of the crown projection (DC), leaf length (CL), leaf width (LL), leaf format (FF) and footstalk length (CP). Molecular data were obtained by amplification of eight microsatellite loci. We estimated the following quantitative genetic parameters: heritability and genetic variation coefficient, and the molecular parameters: gene diversity and allelic richness. We compared the probability distributions of the genetic structure parameters for both, quantitative and molecular data (𝑄𝑆𝑇 vs. 𝐹𝑆𝑇). From the quantitative genetic parameters we found modest responses to selection for the traits: AP, CC and DC; and significant responses for CL, LL, FF and CP. It was observed that the samples collected in natural populations are well represented in the germplasm collection, supported by molecular gene diversity. The traits AP, DC and DC are under convergent natural selection, and the traits CL, LL, FF and CP are under divergent natural selection into the cagaiteira subpopulations of Southeast Goiás.Item Qualidade de grãos em feijoeiro-comum: estimativas de parâmetros genéticos, interação com ambientes, eficiência de seleção e correlação entre caracteres(Universidade Federal de Goiás, 2018-06-29) Almeida, Danilo Valente; Melo, Leonardo Cunha; http://lattes.cnpq.br/9132553601896172; Melo, Patrícia Guimarães Santos; http://lattes.cnpq.br/1508679345970114; Melo, Patrícia Guimarães Santos; Melo, Leonardo Cunha; Silva, Fernanda de Cássia; Ribeiro, Nerinéia DalfolloCommon bean (Phaseolus vulgaris L.) is one of the most important crops related to human feeding playing an important role in ensuring food security. Bean is an important source of protein, besides providing considerable amount of fiber and micronutrients as iron and zinc. Thus, the search for genotypes with high nutritional value grains and that meet standards for commercialization is among the objectives of common bean breeding program, specially the carioca grain type one, the preferred, according to the demand for more nutritious and fast foods. Thus, this work has for objectives: I) To investigate the genetic variability and the environment and genotype-environment interaction effects on seed iron (FeC), zinc (ZnC), crude fiber (CF) and protein content (PC), cooking time (CT), grain yield (GY), 100 grain weight (GW), sieve yield (SY), grain color at harvest (GCH) and grain color after 90 days of storage (GCS); II) To evaluate the degree of information of evaluations for grain quality conducted in an environment; III) To estimate genetic parameters and genetic gains for FeC, ZnC, CF, PC and CT; IV) To estimate phenotypic, genetic and environmental correlations between grain quality traits and GY; V) To select advanced common bean lines with carioca grain type superior for grain quality. 81 common bean lines with carioca grain type were evaluated in Preliminary Tests (PT) in triple lattice design 9x9 with plots of two 4-meter rows. In these trials the traits GY, SY and GW were evaluated and FeC, ZnC, CF, PC and CT were evaluated in only one PT. In this trial the phenotypic and genetic parameters and the genetic gains were estimated. The 20 superior lines were selected considering FeC, ZnC, CF, PC and CT and then evaluated in Validation Tests (VT), conducted in 9environments, between 2015 and 2017, in randomized complete block design with 3 replicates and plots of one 4-meters row. Analysis of variance and joint analysis were performed for all the grain quality traits FeC, ZnC, CF, PC, CT, GY, SY, GCH, GCS and GW evaluated on VT. The phenotypic, genetic and environmental correlations were estimated between the traits FeC, ZnC and GY. The phenotypic adaptability and stability parameters were estimated based on the method proposed by Nunes et al. (2005) and the selection of the best genotypes for grain quality was performed based on the index of Mulamba & Mock (1978). The preliminar evaluation of the grain quality traits was efficient and suficient for selecting the superior genotypes for FeC, CF, PC and CT, however, based on the GxE interaction detected, it is recommended to evaluate in at least 2 environments. Significant effects of genotype, environment and genotype-environment were detected for all the traits evaluated on VT. Based on the genetic variability detected, genetic gains can be obtained with the selection for FeC, ZnC, PC and CT, but for CF the available variability is a challenge to obtain important gains. The phenotypic and genetic correlations between FeC and ZnC were significant, wich indicate simultaneous gains for these traits. The lines CNFC 16627, CNFC 16518, CNFC 16602, CNFC 16615 and CNFC 16520 can be used for the improvement of carioca grain quality.Item Variabilidade morfoagronômica da coleção de germoplasma de mangabeira (Hancornia speciosa Gomes) da Universidade Federal de Goiás(Universidade Federal de Goiás, 2015-08-28) Almeida, Gabriella Queiroz de; Telles, Mariana P. Campos; http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4707329E5; Chaves, Lázaro José; http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4783820E2; Chaves, Lázaro José; Peixoto, Nei; Coelho, Alexandre Siqueira GuedesThis study aimed to characterize the Mangaba (Hancornia speciosa Gomes) Germplasm Collection owned by the Universidade Federal de Goiás, by measuring 40 morpho-agronomic characters. The collection was designed in randomized blocks with 57 treatments (progenies) and four replications, totaling 192 individual accesses, which represent 29 natural populations and four botanical varieties. The variance components estimation was done using random model, and REML procedure, considering the effects of botanical varieties, populations within varieties, progenies within populations and individuals within progenies. Considering 40 morpho-agronomic characters, it was observed that there is significant genetic variability in seven characters among the progenies, 24 characters among the populations and 18 characters among botanical varieties. In addition, individual heritability was higher than 50% in 21 of them. The highest percentual individual selection gain was estimated for the character number of fruits produced by each plant. Significant correlation in some biometric characters between young and adult plants was noticed, including their productivity, which suggests the possibility of early selection. Among the 55 genetic correlations among the agronomic characters, 25 were significative. The fruit production peak occurred between June and October, H. speciosa var. cuyabensis being the most productive one. Multivariate analyses results showed that H. speciosa var. speciosa is the most genetically different in comparison with the others. The characters suggested as minimum quantitative descriptors for the Mangaba tree were: leaf length and width, petiole length and diameter, length between the knot, corolla diameter, flower peduncle diameter, fruit length and diameter, number of fruits, plant height and first branching heightItem Eficiência da seleção para qualidade da fibra do algodoeiro (Gossypium hirsutum)(Universidade Federal de Goiás, 2018-11-29) Alvarenga, Luís Gabriel Silva; Silva Filho, João Luis da; http://lattes.cnpq.br/0608088933335198; Resende, Marcela Pedroso Mendes; http://lattes.cnpq.br/2080097211870591; Resende, Marcela Pedroso Mendes; Silva Filho, João Luis da; Tarazi, Roberto; Chaves, Lázaro JoséCotton fiber is the main feedstock used in the world textile industry. It is determined by several traits of complex inheritance, influenced by the environment, and which can be negatively correlated with each other, hindering genetic gains in selection for fiber quality. The aims of this study were to estimate genetic parameters for traits related to cotton fiber quality; propose a graphical method to measure the gain with the selection using different intensities of selection; and to verify the viability of SCI and CSP as selection indices for fiber quality. From the cross between the cotton cultivars BRS 336, conventional with high quality fiber, and DP 164 B2RF, transgenic with medium quality fiber, two populations were carried out: one using the bulk breeding method, and another from the backcross with the cultivar BRS 336. Fiber samples of F5:6 (bulk) and F3:4RC3 (backcross) progenies were analyzed using HVI apparatus, which evaluates the fiber quality by measuring the traits length (C), maturity (MAT), uniformity (U), micronaire (MIC), elongation (ELG), short fiber index (FC), resistance (R), degree of yellowing (b), the spin-consistency index (SCI) and the reliability index (CSP). Genetic variability was observed among progenies for R, MIC, ELG, C and FC, confirming the complexity of these traits. The means of the two populations met the needs of the industry, indicating the presence of genotypes with commercial potential. Estimates of heritability ranged from 3.29% (Rb - F5:6) to 59.92% (C - F3:4RC3). All traits showed genetic correlations with at least one trait, and negative correlation was observed between C and the traits PF, MIC, MAT and FC. The CSI index presented a negative correlation with FP, MAT and FC, and positive correlation with C and R. The SCI index showed a negative correlation with MIC and MAT, and a positive correlation with C. The selection of the 20% best progenies using both indices presented favorable responses for C, R and FC, with expected reduction in FP, and without significant influence on the other traits. The graphs of the correlated response were more informative than the estimates obtained through the equation, since they do not need the values of genetic correlation, and allow the analysis considering several intensities of selection. The graphs of heritability showed that the selection gains were reduced or negative for some traits as the selection intensity increased. The strategy of using the gain graphs using different intensities of selection is efficient and easy to be implemented by breeding programs, especially when the objective of the program is to evaluate complex traits, influenced by other characters, such as cotton fiber quality.Item Métodos de obtenção de novas fontes de resistência à brusone foliar por meio da cultura de tecidos em arroz (oryza sativa)(Universidade Federal de Goiás, 2018-12-10) Alves, Lays Lohanne; Lobo, Valácia Lemes da Silva; http://lattes.cnpq.br/2687017480805879; Sibov, Sergio Tadeu; http://lattes.cnpq.br/4627553641870284; Araújo, Leila Garcês; http://lattes.cnpq.br/9895211901348365; Araújo, Leila Garcês; Lobo, Válacia Lemes da Silva; Castro, Adriano Pereira de; Guedes, Márcio LisboaRice (Oryza sativa L.) is the staple food of more than half of world's population. The productivity in all cropping systems is affected by the blast (Magnaporthe oryzae), the main rice disease, can cause losses of up to 100% depending on climatic conditions and the virulence of the pathogen. Obtaining new sources of blast resistance is very important for the management of this disease. Double haploid populations allow acceleration of lineage fixation as allows homozygotes to be achieved in just one generation. Somaclonal variation is another tissue culture technique successfully used for the induction of blunt mutations. The objective of the present work is to obtain new sources of resistance to leaf blast in populations of double haploids and somaclones. For this, callus induction and plant regeneration were performed from the anthers of BRS Primavera x CNA 923 and immature panicles of the cross between the cultivars Metica-1 and Cica- 8.In a greenhouse, R2 plants were inoculated with the IB-1 and IB-45 pathotypes of Magnaporthe oryzae, from the cultivars Metica-1 and Cica-8, respectively. For each pathotype a population of 800 plants was used. On the seventh day after inoculation, leaf blast severity was evaluated using the scale of scores ranging from 0 to 9, with a score of 0 to 3, representing resistance reactions and 4 to 9, susceptibility reactions. The severity of the blast was evaluated in a population of 50 plants on generation R2 somaclones with each parent (Metica-1 and Cica-8) and their respective M. oryzae races (IB-1 and IB-45). For severity, four evaluations were performed at 48-hour intervals to calculate the area below the disease progress curve (AACPD). The data were submitted to the T test in the SPSS program. Of a total of 800 plants inoculated with the IB-45 breed, 644 plants were resistant and 156 showed susceptibility reactions. For the IB-1 breed, 664 resistant and 136 susceptible R2 plants were obtained, indicating the induction of genetic variation in relation to leaf blast resistance in the initial generations. The T-test showed a significant difference in the severity of blast between the parents and the somaclones, with a mean of 32.96% for the Metica-1 cultivar and 3.36% for the somaclones inoculated with the IB-1 breed, and 34,24 % for the cultivar Cica-8, in contrast whit 7.59% for the somaclones inoculated with the IB-45 breed. There was a significant reduction of the AACPD, with a mean of 56.76% for Metica-1 and 6.22 for the somaclones inoculated with the IB-1 race and 66.83% for the Cica-8 and 12.1 for the somaclones inoculated with the IB -45. There was a significant the somaclones inoculated with the two races, demonstrating that somaclonal variation produces sources of resistance to the pathogen. The R2 plants that presented resistance reaction to the two races were selected and transplanted for generation advance aiming at new sources of blast resistance, thus contributing to the rice breeding program.Item Desenvolvimento de marcadores ambientômicos para arroz de terras altas (Oryza sativa L.) em território brasileiro(Universidade Federal de Goiás, 2023-06-30) Bahia, Marco Antônio Marcelino; Teixeira, Sônia Milagres; http://lattes.cnpq.br/1596947832723945; Sette Júnior, Carlos Roberto; http://lattes.cnpq.br/6511764239807115; Resende, Rafael Tassinari; http://lattes.cnpq.br/7248301627149286; Resende, Rafael Tassinari; Melo, Patrícia Guimarães Santos; Zaidan, Úrsula RamosRice (Oryza sativa L.) is one of the staple foods in the Brazilian diet, and therefore, its cultivation and productive independence are strategically essential for ensuring the population's food security. Within rice farming, selecting the appropriate genotype for planting is the factor that most strongly impacts the outcome of the endeavor. In order to support this decision-making process, enviromics has been applied with the objective of selecting genotypes with higher productive potential for specific areas of interest. The aim of this study was to generate and analyze the contribution of enviromic markers to the total upland rice production data in Brazilian territory. The experimental data were provided by Embrapa Rice and Beans and involved the evaluation of 2,119 rice genotypes in 187 municipalities or localities across the country, spanning the period from 1982 to 2018. For the generation of enviromic markers, data from the SoilGrids, WorldClim, and NASA POWER platforms were used, resulting in a total of 393 environmental covariates collected. The generation of enviromic markers was performed using the Monte Carlo method, with 10,000 iterations and always considering the presence of the 187 municipalities where the Embrapa experiments were conducted. The Random Forest package and the IncMSE and IncNodePurity methods were used to evaluate the importance of each covariate for the model applied throughout the Brazilian territory. The results showed that the coefficient of variation for seasonal precipitation was the most important covariate for both models.Item Desenvolvimento de marcadores microssatélites para Stryphnodendron adstringens (barbatimão - Fabaceae)(Universidade Federal de Goiás, 2018-08-27) Barateli, Luciana Oliveira; Telles, Mariana Pires de Campos; http://lattes.cnpq.br/4648436798023532; Brito, Cíntia Pelegrineti Targueta de Azevedo; Soares, Thannya Nascimento; Telles, Mariana Pires de CamposStryphnodendron adstringens presents a wide geographical distribution, being predominant in regions of Cerrado sensu stricto and is popularly known as "barbatimão". It is a species widely used in herbal medicine because of its anti-inflammatory, antibacterial and antiulcerogenic potential. The Cerrado’s removal of vegetation cover reduces significantly the number of several native species, among them S. adstringens. Thus, it is important that efficient strategies for the use and conservation of this species are drawn. In order to provide molecular tools for studies of genetic diversity and conservation of S. adstringens, the present work had the objective of developing microsatellite markers for the species. Genomic DNA was obtained from leaf tissue using the CTAB protocol. The identification of the microsatellite regions and the design of the primers were performed using the QDD program modules. From the identified microsatellite regions, 20 pairs of primers were designed, 14 of which flank microsatellite regions composed of dinucleotides, four by tetranucleotides and two by pentanucleotides. Initially, four individuals were used for the standardization tests of the PCR protocol and annealing temperatures. Subsequently, 48 individuals were selected, distributed in three populations, to evaluate polymorphism via 6% polyacrylamide gel. Of the 20 pairs of primers evaluated, 16 presented polymorphic amplification products and four monomorphic amplification products. Considering the 16 polymorphic markers, the number of alleles varied between two (SadH19) and 13 (SadH13), with a mean of seven alleles per locus. The observed heterozygosity (Ho) and expected (He) and PIC values were 0.506, 0.543, 0.635, respectively. The mean Hmax value founded (65,519) indicates values of genetic diversity that can be considered medians for this set of loci evaluated in three populations of S. adstringens. On the other hand, although genetic diversity is median, this set of 16 polymorphic markers exhibited a ombined probability of paternity exclusion high (0.9999983) and combined probability of genetic identity low (3,49x10-15). The analysis of variance of allelic frequencies presented significant values for two of the three estimated statistics with f not significant 0.050, significant θ equal to 0,329 and F in the overall value also significant 0.360. Thus, it can be concluded that the panel of polymorphic markers developed for S. adstringens is highly informative and indicated for population genetic studies for the species. Another important factor is that these markers can be tested in other evolutionarily close species for the availability of microsatellite markers, without the need to develop new primers.Item Progresso genético para produção de grãos obtido em doze anos de melhoramento da população elite de arroz de terras altas(Universidade Federal de Goiás, 2015-08-10) Barros, Matheus Souza de; Morais, Orlando Peixoto de; http://lattes.cnpq.br/2175152793170778; Melo, Patrícia Guimarães Santos; http://lattes.cnpq.br/1508679345970114; Melo, Patrícia Guimarães Santos; Morais, Orlando Peixoto de; Torga, Paula Pereira; Silva Filho, João Luiz daThe genetic improvement resulted from plant breeding acts decisively in maintaining the supply of agricultural foods like rice. The contribution of this genetic improvement to increase productivity is expressed in many crops by genetic progress which represents the genetic changes in the course of the selection cycles, and promotes the advance of genotypic average towards selection. Among the strategies adopted to increase the gain by selection, the early generation selection proves to be promising. This work has aimed to evaluate the genetic potential for selecting upland rice progenies and estimate the genetic progress for grain yield. The data used in this study were obtained from the progeny yield trials conducted in the period of eleven agricultural years 2002/03 to 2012/13 conducted by Embrapa Rice and Beans. The traits analyzed were grain yield (kg ha-1), plant height (cm) and days to flowering (day). In each year of the experiment, a group of progeny was tested in four to six sites. It was used Federer's augmented block design without replication per site in seven years and with at least three checks, in four years of the series were used two replications. The experimental data within each year were submitted to joint analysis. A mixedeffects linear model was applied for estimating the components of variance by the method of restricted maximum likelihood (REML). From this estimate of the components, it was calculated the genetic and phenotypic parameters, in addition the selective accuracy, the experimental precision coefficient, the experimental variation coefficient and relative variation coefficient. The genetic progress was estimated by the method of generalized linear regression of the adjusted means of progeny groups evaluated in each year by the mixed model approach. The estimates of the relative annual mean gain and the total relative gain for the three studied traits were also obtained. The estimates of genetic variance among progenies for grain yield were highly significant (p < 0.001), except for the progeny group evaluated in 2007. The heritability estimation ranging from 0.22 to 0.69 that, associated with the selective accuracy, indicates the expected level of efficiency with early generation selection in each group of progenies. For Plant height and daysto- flowering, the genetic variance estimates were significant (p ≤ 0.01) in all groups. The variance components for these traits led to a rather high heritability estimation that suggests favorable conditions for selection in early generations. The genetic progress for grain yield (80.5 kg ha-1 yr-1) was highly significant. This value represents a relative annual mean gain of 2.88%. Throughout the period, the cumulative gain was estimated in 32.86% which indicates an increase of 918 kg ha-1 for grain yield. The response for plant height was not significant, suggesting that the height of progenies remained stable over the period. For days-to-flowering, it was detected significant increase in cycle length, indicated by the cumulative increase of the vegetative period in about five days (6.73%). From these results we conclude that early generation selection, adopted by the breeding program, were effective in promoting the genetic gain for grain yield in the elite populations.Item Mapeamento de QTL para componentes de produção em arroz sob duas condições de irrigação(Universidade Federal de Goiás, 2012-08-30) Benício, Cristyene Gonçalves; Brondani, Claudio; http://lattes.cnpq.br/4775600104554147; Brondani, Claudio; Borba, Tereza Cristina de Oliveira; Coelho, Alexandre Siqueira GuedesRice (Oryza sativa L.) has great social and economic importance worldwide. Produce food for a growing world population, promoting increased productivity in environmentally appropriate conditions, it one of the great challenges of breeding programs. Rice production based on seasonal rainfall, typical of upland rice (rainfed), today represents about 40% of Brazilian production. The agricultural irrigation consumes much of the planet's fresh water, and restricting the use of water resources is a reality. With the rising cost of water for agriculture and potential shortages in some regions of the planet, the development of plants more efficient in the water use is a priority demand of breeding programs. This study aimed to evaluate and compare the polymorphism of a set of microsatellite markers (SSRs) and SNPs (Single Nucleotide Polymorphisms) and use them for mapping QTL for yield components under two irrigation conditions. The parentals Douradão (drought tolerant) and Primavera (susceptible to drought) resulted in a segregating population consisting of 221 F2:5, which were genotyped with fluorescent SSR markers in analyzer ABI3100 (Applied Biosystems) and SNP markers developed for the GoldenGate platform based on Veracode technology (Illumina). Among the 86 SSRs, 11 (12.8%) did not amplify and 41 (54.7%) were polymorphic. Among the 1920 SNPs, 316 (16.45%) did not amplify for both parentals and 46 (2.87%) were polymorphic. The parentals and their progeny were evaluated in two trials (with and without water deficit) in 12x19 rectangular lattice design with two replications. The composite interval mapping analysis identified 53 QTL, 10 of which related to ISS (index of susceptibility to drought) and five to productivity in water stress condition. Among the identified QTL it were found putative genes related to plant abiotic stress defense mechanisms. Families CNAx15128-70-B, CNAx15128-118-B, CNAx15128-74-B and CNAx15128-120-B showed higher yield under drought and lower ISS. These families may be evaluated by rice breeding programs aiming the development of superior cultivars.Item Diversidade genética de BGMV em linhagens elite de feijoeiro-comum(Universidade Federal de Goiás, 2021-06-30) Bertholdo, Naíze Motta; Faria, Josias Corrêa de; Coelho, Alexandre Siqueira Guedes; http://lattes.cnpq.br/0840926305216925; Coelho, Alexandre Siqueira Guedes; Faria, Josias Corrêa de; Dianese, Érico de Campos; Araújo, Leila Garcês deBrazil is the main common bean consumer in the world, also being the main producer, whose production supply the intern market. Among the factors that influence the productivity, the wide distribution of Bean golden mosaic virus in common bean fields is very important because it can cause chlorosis, leaf crumpling and stunting, and lead up to 100% yield losses. As a response to that, Embrapa developed, through the RNAi approach, a transgenic common bean line with resistance to the Bean golden mosaic virus. Nonetheless, the presence of a resistance gene can increase the selection pressure over the pathogen, that can mutate and overcome the resistance. Despite being DNA viruses, it is described that in species of Begomovirus, the genus of the BGMV, mutation rates are similar to those of RNA viruses. Nevertheless, until now, BGMV has shown smaller genetic variability compared to other species of the genus. The objective of this work was to characterize BGMV populations that infect different common bean elite lines, transgenic and conventional, in two locations: Brasília and Santo Antônio de Goiás. To that, leaf samples from different elite lines were collected in Value for Cultivation and Use (VCU) trials, searching for differences in viral populations from different sources. We used a strategy widely used on studies related to other living beings: next generation DNA sequencing followed by single nucleotide polymorphisms (SNPs) genotyping. The SNPs obtained were used to carry out genetic diversity analysis. The estimates of the proportion of polymorphic sites and of Nei’s gene diversity revealed a greater genetic diversity in viral populations sampled in transgenic plants. The results suggest that, despite the restriction to the viral replication and, therefore, of smaller viral populations, the necessary conditions to the occurrence of selection pressure are given. In the genetic structure analysis, a significant effect was attributed to localities, as has been widely described in literature.Item Avaliação da tecnologia Oxford Nanopore para análise de identidade genética de clones de cana-de-açúcar (Saccharum spp.)(Universidade Federal de Goiás, 2020-03-31) Borges, Sâmella de Souza; Bandeira, Ludmila Ferreira; http://lattes.cnpq.br/5329718658913234; Coelho, Alexandre Siqueira Guedes; http://lattes.cnpq.br/0840926305216925; Coelho, Alexandre Siqueira Guedes; Novaes, Evandro; Borba, Tereza Cristina de Oliveira; Taquary, Adriana Maria Antunes; Vianello, Rosana PereiraBrazil is currently the world's largest producer of sugarcane, which is the raw material for two important products for the Brazilian economy: sugar and ethanol. Aiming both to assess the Oxford Nanopore technology for genotyping applications for sequencing (Genotyping By Sequencing - GBS), with the differential to obtain a high sequencing coverage (> 1,000x), how to develop a GBS platform to to identify sugarcane clones, safely and conveniently, this work used the MinION platform to perform genotyping based on the sequencing of 48 individuals. For this, using the genome of Sorghum bicolor as reference and a set of libraries of sugarcane transcripts, 20 pairs of primers were designed, which were used to obtain amplicons, in which SNPs molecular markers were identified (Single Nucleotide Polymorphisms). Six sequencing libraries were built, the first two being used in pilot trials. The alignment of the sequences obtained in the reference genome was performed using the BWA program. The identification and genotyping of the SNPs was performed using the SAMtools software. The identification of the sugarcane clones was done by calculating the genetic distance between individuals. The cluster analysis was performed using a script written in software R. The sequencing resulted in approximately 841 thousand sequences. The average size of the amplicons was 1.6 kb. High sequencing coverage (average 10,498X / amplicon) was obtained. Nine amplicons were selected, in which 356 SNPs sites were identified. The percentage of mismatches between the obtained and the reference sequences varied from 8% to 20% and the percentage of indels remained homogeneous (~ 6%). The duplicates of the same individual used as biological control formed a knot with a consistency of 94% in the obtained dendrogram, however they did not present perfect genetic identity between them. It is suggested that this fact is mainly associated with the high rate of sequencing error of the Oxford Nanopore sequencing technology, evidencing the difficulty of its use in applications that require a genetic identification with a high degree of security, as occurs in problems involving clonal identification in sugar cane.Item Interação genótipo x ambiente em soja com ênfase na estratificação ambiental para a região central do Brasil(Universidade Federal de Goiás, 2011-12-19) Branquinho, Rodrigo Gomes; Duarte, João Batista; http://lattes.cnpq.br/4117228759548186; Duarte, João Batista; Chaves, Lázaro José; Melo, Patrícia Guimarães Santos; Pacheco, Roberto MirandaThe objective of this study was to establish a consistent environmental stratification for the region of soybean cropping in Central Brazil, based on genotype by environment (GE) interaction analysis. For this, yield data from variety trials conducted by Embrapa Cerrados in partnership with others Brazilian institutions, during seven growing seasons (2002/03 to 2008/09), were used. The study covered six experimental sets that were related to the genotypes of three maturity groups (early, medium and late), and two commercial groups (soybean conventional and transgenic RR), totaling 559 trials analyzed. The statistical treatment of data was performed in two stages: first, analyses of variance were performed for each experiment, from which the estimates of treatment mean (combination of genotype and environment) were obtained. In the second stage the joint and GE interaction analyses were performed. Thus, the yield mean of each genotype in each environment were submitted to the AMMI analysis (Additive Main effects and Multiplicative Interaction model), that led to choose a model with only one principal component (AMMI1). As result of this analysis, the genotypes and environments were jointly represented in a scatter plot called biplot (graph that display the rows and columns of a matrix; in this case, genotypes and environments are marginal in this table). To stratify the target region, the approach of winner genotypes (Gauch & Zobel, 1997; Crop Sci. 37: 311-326) was used. In this approach each stratum is composed by locations that shared a same winner genotype (one that is the higher yielding mean ranking of a location). In the AMMI1 biplot, the boundaries of each stratum were identified by horizontal lines drawn from the ordinate points (scores) corresponding to the environment of transition between two strata, which are characterized by their winner genotypes. With this information, the environmental strata were established for each growing year and experimental set. The maturity groups of assessed lines determined the environmental stratification obtained. Thus, the following locations were grouped to other localities, presenting a characteristic of redundancy: a) early maturity group (seven strata): (Campo Novo do Parecis, Maracajú, São Miguel do Araguaia, Tangará da Serra); (Conquista, Nuporanga, Sidrolândia, Sorriso); (Cristalina, Iraí, Sacramento); (Montividiu, Sonora, Tapurah); (Capinópolis, Senador Canedo); (Guaíra, Morro Agudo); and (Lucas do Rio Verde, Sapezal); b) medium maturity group (four strata): (Anápolis, Montividiu, Tangará da Serra); (Barreiras, Campo Novo do Parecis, Uberaba-Chapadões); (Chapadão do Sul, Conquista, Maracajú, Sonora); and (São Gabriel, Sorriso, Uberaba-Epamig); c) late maturity group (five strata): (Campo Novo do Parecis, Planaltina, Senador Canedo, Tapurah); (Iraí, Sacramento, Sonora); (Lucas do Rio Verde, Sorriso); (Goiatuba, Tangará da Serra); and (Barreiras, São Desidério). Were also identified key-locations to conduct the trials in the final stage of genotypic evaluation (advanced variety trials): a) early maturity group: Anápolis, Barretos, Campos de Júlio, Capinópolis, Chapadão do Céu, Chapadão do Sul, Goiatuba, Igarapava, Jataí, Luziânia, Morro Agudo, Planaltina, Primavera do Leste, Sacramento, São Gabriel do Oeste, São Miguel do Araguaia, Sapezal, Sidrolândia, Sonora, Uberaba-Chapadões, Uberaba-Epamig e Unaí;b) medium maturity group: Barreiras, Barretos, Campo Alegre, Campos de Júlio,Capinópolis, Chapadão do Céu, Chapadão do Sul, Cristalina, Goiatuba, Iraí, Jataí, Lucas do Rio Verde, Luziânia, Montividiu, Perolândia, Planaltina, Primavera do Leste, Rio Verde, Sacramento, São Desidério, Senador Canedo, Sorriso e Unaí; c) late maturity group: Anápolis, Campo Alegre, Campo Novo do Parecis, Campos de Júlio, Capinópolis, Chapadão do Céu, Chapadão do Sul, Cristalina, Goiatuba, Jataí, Luziânia, Montividiu, Primavera do Leste, Rio Verde, São Desidério, São Gabriel do Oeste, Sonora, Sorriso, Uberaba-Chapadões, Uberaba-Epamig e Unaí. Finally, among the locations recommended for the network of advanced trials, one was also appointed as key-location to conduct the initial stages of genotypes assessment in each maturity group. The locations Campos de Júlio (to early group), Rio Verde (medium and late groups) were in order indicated because resulted the best rankings of the winner genotypes through the target region.Item Estabilidade e potencial produtivo de linhagens de feijão-comum desenvolvidas sob fixação biológica de nitrogênio avaliadas em multi-ambientes(Universidade Federal de Goiás, 2023-10-31) Caetano, João Pedro Miranda; Melo, Leonardo Cunha; http://lattes.cnpq.br/9132553601896172; Melo, Patrícia Guimarães Santos; http://lattes.cnpq.br/1508679345970114; Melo, Patrícia Guimarães Santos; Vidotti, Miriam Suzane; Dias, Polianna Alves Silva; Melo, Leonardo CunhaChemical nitrogen fertilization is a common practice in bean fertilization, but it is expensive and can have negative environmental impacts, as well as synthetic fertilizer not being a renewable source. Biological nitrogen fixation (BNF) is one of the most sustainable ways of supplying legumes with nitrogen. In the last two decades, BNF has returned to the focus of common bean breeding programs, mainly by evaluating the potential of strains for BNF in various environments, with a view to recommending adapted and stable genotypes. The aim of this work was to evaluate the performance and productive stability of elite common bean lines of the carioca group developed under BNF. The lines were obtained by crossing the genitors CNFC10762 and the wild accession G234999A, which has a high BNF rate but few favorable agronomic characteristics. After backcrossing, the progenies that were advanced and selected under BNF were obtained. The 15 elite lines (L) obtained and three check genotypes were evaluated in six environments, made up of the combination of location/harvest/year. Two experiments were conducted in each environment, one with nitrogen fertilization of 120 kg ha-1 of nitrogen through mineral fertilizers and the other inoculated with a mixture of Rhizobium tropici and R. freirei without mineral nitrogen fertilization. The experimental design was randomized blocks with three replications, and the plots were made up of three three-metre rows spaced 0.5 m apart. Individual and joint analyses of variance were carried out for grain yield and the stability analysis was done using the WAASB method, which combines the predictive capacity of the BLUP mixed model with the graphical tools of the AMMI model. The effect of the L x FN interaction was significant (P<0.01), with the genotypes performing better when there was nitrogen fertilization. Among these strains, CNFC10762 and CNFC20367 had the best performance and productive stability when both N sources were considered, i.e. they have dual aptitude characteristics and are adapted to both mineral N and BNF systems. For inoculated environments, the control CNFC10762 and the genotypes CNFC20389, CNFC20367 and CNFC20379 stood out, showing the best relationship between productive performance and stability. Common bean breeding was unable to develop lines, originating from wild germplasm, that are more productive than the controls genotypes developed under an exclusive system of mineral N fertilization. The lines from this work will be indicated as progenitors to form new breeding base populations.Item Caracterização genética de uma população base do programa de melhoramento de cana-de-açúcar da Ridesa/UFG(Universidade Federal de Goiás, 2017-12-21) Carneiro, Karla da Silva; Coelho, Alexandre Siqueira Guedes; http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723538Z2; Coelho, Alexandre Siqueira Guedes; Nunes, Camila de Marillac Costa; Lopes, LorennaIn Brazil, the history of sugarcane is related to the social, economic and politic country development. Sugarcane cultivation is considered as the first organized economic activity in the country. The main purpose of sugarcane cultivation is for sugar and biofuel production, but in recent years the energy production from its biomass has also been explored, increasing the attention for this crop. Modern cultivated sugarcane varieties are hybrids from interspecific crosses between S. officinarum and S. spontaneum. The genetic breeding has given many contributions to sugarcane production and exploration, by the development of superior genotypes. The main sources of variability used in breeding programs are the germplasm banks. However, to explore these resources efficiently it is necessary to have basic information on the available levels of genetic diversity and on its structure, to support decisions on how they can be used in breeding programs. The purpose of this work was to characterize the genetic diversity and structure of a base population from the Ridesa/UFG sugarcane breeding program. A sample of 160 sugarcane clones were genotyped using 37,914 SNP markers. The population showed medium levels of genetic diversity. The average Nei’s gene diversity index was estimated to be 0,173, while the medium observed heterozygosity was a little higher (0,236). The genetic divergence, estimated by Roger’s modified distance varied from 0,20 to 0,30. SNP markers were efficient to identify individuals that are genetic divergent or similar, even without genealogy information. The population structure analysis, performed with the software Structure, suggested the existence of two clusters. Each clone had a fraction of its genome inside these two clusters, corroborating the fact that modern sugarcane cultivars are essentially hybrids. Our results suggest that, given the low level of genetic structure among clones, from the breeding programs standpoint, the evaluated population can be managed as weakly structured, although some small groups, including a small number of clones, had been detected. Among the evaluated clones, the least divergent pairs were those formed by the genotypes 023 and 011, and 066 and 036. The most divergent pairs were formed by the clones 131 and 084, and 131 and 063.Item Antagonismo entre Magnaporthe oryzae e o fungo micorrízico Rhizoctonia sp.(Universidade Federal de Goiás, 2013-03-25) Carvalho, Jacqueline Campos Borba de; Kato, Lucília; Araújo, Leila Garcês de; http://lattes.cnpq.br/3488880262260757; Araújo, Leila Garcês de; Kato, Lucília; Lobo, Valácia Lemes da Silva; Filippi, Marta Cristina da Corsi diRice blast caused by the fungus Magnaporthe oryzae B. Couch [anamorfo - Pyricularia oryzae Cav.] occurs in all rice growing regions of the world. The sustainable agriculture requires the introduction of biological control as one of the components in the integrated disease management. The microorganisms associated to plants are capable of producing secondary metabolites such as alkaloids which may have a role in biological control. The objective of the present study consists, isolation and identification of secondary metabolites of the micorrhizal fungus Rhizoctonia sp. obtained from Epidendrum nocturnum and evaluate in vitro and in vivo antagonism to M. oryzae. Ten fungal isolates were used to test the antibiosis against M. oryzae. The isolate En07 of Rhizoctonia sp. exhibited a greater halo of inhibition and consequently was considered the best in vitro antagonist to M. oryzae. Crude, mycelial and lyophilized extracts of micorrhizal isolate were obtained. The analysis by CCD of these three extracts showed positive results in relation to Dragendorff, indicating the presence of phenolics. The analysis of RMN 1H and masses showed the presence of aromatic hydrogens and phenolics. Five concentrations of each extract were prepared and utilized in the studies on in vitro mycelial inhibition of M. oryzae and observed 77.86% of pathogen reduction by crude extract (700 μg/mL). Two crude extract treatments (520 μg/ml and 120 μg/ml) significantly reduced the radial growth of the pathogen compared to control. The crude extract showed best results for mycelia inhibition of the pathogen, followed by lyophilized and mycelial extracts. In two trials, the crude extract at 0.52 μg.μL-1 also reduced the formation of appressoria of M. oryzae by 100%. Two greenhouse experiments were conducted on leaf blast suppression with the cultivar Primavera, using completely randomized design with three replications. In both these trials, the mycelial extract (1860 μg/ml and M.o 3x105) showed marked reduction of leaf blast severity in relation to control by 59.27% and 77.58% respectively. In the second trial, the second treatment (1040 μg/mL and M.o3x105) of crude extract reduced AUDPC by 64.63% compared to control. The results showed that the metabolites of Rhizoctonia sp. posses great potential for biological control of rice blast.Item Depressão endogâmica e variabilidade genética de populações para produção de milho verde(Universidade Federal de Goiás, 2020-03-30) Cascão, Luma Mariano; Resende, Marcela Pedroso Mendes; http://lattes.cnpq.br/2080097211870591; Melo, Patrícia Guimarães Santos; http://lattes.cnpq.br/1508679345970114; Melo, Patrícia Guimarães Santos; Resende, Marcela Pedroso Mendes; Vidotti, Mirian Suzane; Reis, Edésio Fialho dosThe production of vegetable corn is an activity of great importance for several regions of Brazil. Despite this, breeding programs have devoted little effort to developing and recommending cultivars specifically for this purpose. Then, it is essential that programs for the improvement of public institutions seek to serve this niche market, which has a relevant social role, as it is conducted mainly in small areas. This work aimed to estimate inbreeding depression and the genetic components of three populations for the production of vegetable corn. The populations evaluated have a different genetic basis: a variety of open pollination, called UFG-Samambaia (P1), a population obtained by crossing two older hybrids (P2) and a third population obtained by crossing of the modern hybrids (P3). Progenies of these three populations were obtained by sampling self-fertilized S0 plants. S1 progenies, three S0 populations and two checks were evaluated in a triple lattice 14 x 14 with plots of a four meter line. They were evaluated the agronomic traits: ears weight, plant height, ears height, relative position of the ear, breaking and lodging plants, days to anthesis, days to silking; and quality traits of ears: mass weight, ears weight without straw, length of ears without straw, ears diameter, ear alignment, ear stuffing, grain color. Statistical analyzes were performed using a mixed model approach. Inbreeding depression was estimated for all characters and the components of means (m + a ’and d) and variance. Genetic variability was observed for most traits among P1 progenies. The progenies of the P2 and P3 populations did not show genetic variability for most characters. The three populations suffered an impact when subjected to self-fertilization due to the effects of inbreeding. Inbreeding depression in P1 has higher rates for most characters: plant height, ear height, relative ear position, mass weight, length of ears without straw, alignment of ears and stuffing; In P2, the male flowering characters, weight of ears without straw and diameter of ears show a higher index and in P3, the characters of ear weight, female flowering, breakage and lodging and grain color. In general, the quality characters of the ear, important for the production of green corn, the greater depression due to inbreeding than the agronomic characters. This shows that, inbreeding depression must be considered in the selection process, with the frequency of favorable alleles increasing throughout the improvement process.Item Capacidade de combinação de híbridos simples de milho e interação com épocas de plantio(Universidade Federal de Goiás, 2020-07-30) Castro, Laís Lopes de; Guedes, Márcio Lisboa; http://lattes.cnpq.br/8695921024674566; Melo, Patrícia Guimarães Santos; http://lattes.cnpq.br/1508679345970114; Resende, Marcela Pedroso Mendes; http://lattes.cnpq.br/2080097211870591; Resende, Marcela Pedroso Mendes; Reis, Edésio Fialho dos; Souza, João Cândido de; Melo, Patrícia Guimarães SantosThe high yield obtained in the corn crop and the increases in world production are due to the advances obtained with the genetic breeding. The high productivity obtained in the corn crop and the increases in world production are due to the advances obtained with the genetic improvement. Within the breeding programs, one of the most important decision-making is the formation and choice of the base populations used in the breeding, as they are the ones that will extract the superior strains, so they must gather favorable alleles for characteristics of interest, and for that it is necessary to include several parents in the genealogy of a population. A good strategy for the formation of base populations is the identification of simple high-performance hybrids with high combining capacities for the characteristics of interest. The objective of this study was to identify promising parents and crosses for population formation for the UFG maize breeding program, considering four planting seasons. For the identification of the best parents to compose the new base population, they were evaluated in Goiânia-GO in four planting seasons during the 2018/2019 harvest under a complete diallel genetic design, 6 parents (simple hybrids) and 15 crosses (double hybrids) totaling 21 treatments in the diallel scheme and 5 other controls for comparison purposes. As the behavior of the genotypes is greatly influenced by environmental variations, the analysis of genotype interaction with environments like GGE Biplot was performed. The plant height and ear height characters can be used for selection in the four environments based on the CGC values. There was no pattern of behavior for the determination coefficient estimates (R2) over the four experiments. Parent G1 was better for decreasing the corn cycle with negative flowering values, but in general it presented poor values for CGC and CEC. Parents G2, G3 and G4 showed the best CGC values for the primary production components, and parent G5 as the most stable, although with CGC below the general average. The G1 x G2 cross obtained the highest CEC and at the same time was the most unstable. The G1 x G4, G3 x G6 and G1 x G6 crosses were the most stable and with CEC values higher than the general averagItem Caracterização de híbridos e associação entre caracteres para produção de silagem em milho(Universidade Federal de Goiás, 2019-07-18) Ciappina, Angelina Luzia; Zacaroni, Ozana de Fátima; http://lattes.cnpq.br/4582215859105753; Resende, Marcela Pedroso Mendes; http://lattes.cnpq.br/2080097211870591; Silva, Adelmo Resende da; Silva, Wilton Ladeira; Resende, Marcela Pedroso MendesThe corn hybrids available on the market do not have all the desired traits for making silages with good nutritional value and high productivity. To carry out an improvement breeding program achieve these objectives, it is necessary to characterize the genotypes that can form the base populations of the programs and to understand the interrelationship between the various characters of interest to draw up the best strategies improvement. The objectives of the present work were: i) to perform the agronomic and bromatological characterization of maize hybrids, aiming at the identification of superior genotypes that may comprise base populations of maize breeding programs for silage; ii) to study the interrelationship between characters agronomic and bromatological conditions in maize. Twenty one commercial maize hybrids were evaluated at the Escola de Agronomia da Universidade Federal de Goiás, Goiânia-GO, in the 2017/18 harvest, in two experiments, using the conventional planting density of 55,500 plants.ha -1 , and with a density of 111,000 plants.ha -1 . The experimental design was a randomized complete block with three replications, of a 4.00 m line with twenty plants spaced at 0.20 m. The line spacing was 0.90 m for the standard density and 0.45 m for the densified experiment. The evaluated characters were: male flowering; female flowering; range of flowering; plant height; ear height; ear placement; kernels per ear; number of grains per row; ear diameter; corncob diameter; grain length; ear length; ear weight; weight of one hundred grains; acidity (pH); dry matter content; mineral content; organic matter content; crude protein; neutral detergent fiber (NDF); acid detergent fiber (ADF); total digestible nutrients (TDN); green matter productivity and yield of matter dry. For the experiment with conventional planting density, genetic and phenotypic correlations between the two-to-two traits were estimated, and the direct and indirect effects of these traits on dry matter yield and TDN content. Subsequently, a canonical correlation analysis was performed between the group of characters related to productivity and the group of characters related to silage quality. The increase in density resulted in increase in plant height and a reduction in the size of the ear and biomass produced per plant, however, resulting in higher production per hectare. The AS1596 and AG1051 hybrids presented the best performance, and can be used in the formation of base populations in maize breeding programs for the production and quality of silage, along with hybrids DKB310, BM3061, SHS7920, DKB390, P4285 and RB 9110. A strong relationship was identified between ear components and biomass productivity, and plants with higher grain yields can be used in the indirect selection for dry matter yield. The fiber contents presented a negative correlation with the digestibility of silage and positive with plant height. Knowledge of the indirect effects of the characters between them is important so that the breeder does not make hasty decisions based solely on the correlation estimate. Finally, it is possible to improve maize to simultaneously increase the productivity and the quality of the silage.Item Estrutura genética intrapopulacional e dispersão de pólen em Hancornia speciosa Gomes (Apocynaceae)(Universidade Federal de Goiás, 2013-03-23) Costa, Camila Fernanda; Collevatti, Rosane Garcia; Telles, Mariana Pires de Campos; http://lattes.cnpq.br/4648436798023532; Telles, Mariana Pires de Campos; Collevatti, Rosane Garcia; Chaves, Lázaro José; Martins, KarinaHancornia speciosa (Apocynaceae) is a fruit species which has a wide distribution in areas of Cerrado vegetation type restricted sense. Its flowers have a complex pollination mechanism and unique, co-adapted to pollination by moths and butterflies and their fruits are dispersed by mammals large and medium-sized. It has high economic potential and its use has been conducted in an exploratory way. To implement conservation programs, breeding and enabling the commercial use of species, knowledge about the genetic variability, the spatial genetic structure, the reproductive system and gene flow in natural populations are needed. In this context, in the present study was performed the molecular characterization of the genetic variability in three stages of individuals (adults, juveniles and seedlings) and the assessment of genetic structure spatial (adults and juveniles), of the system of crossing and of the gene flow via pollen in a subpopulation of Hancornia speciosa located in the State Park of Serra de Jaragua, Jaragua-GO. To this end, 113 adults and 100 juveniles were sampled and georeferenced in an area of approximately 2.5ha. Of the total number of adults, 20 trees were selected matrices to obtain seedlings and formation of families of open pollination. Genomic DNA was obtained from the leaves of all individuals (adults, juveniles and seedlings) and was amplified with the use of seven microsatellite loci for obtaining of genotypes. The analyzes of genetic diversity, of spatial structure, rates of cross-fertilization and distance of dispersal of pollen were obtained from these genotypes. The total number of alleles at seven loci evaluated was 125, with an average of 17.8 alleles per locus. For adults the mean allele was 15.8, for the juveniles was 13.5and the seedlings were 11alleles. The average total values of heterozigosidade expected (He) and observed (Ho) were equal to 0.750 and 0.698, respectively. In adults He = 0.750 and Ho =0.714, in juveniles He =0.744 and Ho =0.679 and in seedlings He = 0. 712 and Ho =0. 763. These values indicate that the subpopulation evaluated presents high levels of genetic diversity. The fixation index(f) waspositive and significantforthe generations ofadults(0.052, p <0.05)andjuvenile(0.087, p <0.05), indicating the existence of inbreeding in this subpopulation. The analysis of spatial autocorrelation evidenced that kinship is weakly related to the geographical distance in in bothstagesof lifeevaluated (adults: b= -0.00223, R2 = 0. 000514, p < 0.05 and juveniles: b: - 0.00440, R2 = 0.00148489; p < 0.001).Concomitantly the values of Sp were low and the size of neighborhoods (Nb) were high for the two generations. This result shows that there is no restriction of gene flow via seed and corroborates the hypothesis that the dispersal by animals have high potential to disperse the seeds over long distances.The rates of crossfertilization multilocus (tm= 1.000) and single locus (ts = 0.972 a 1.29) were high and significantly different from zero in all families. The difference in the rate of the crossfertilization multilocus and single locus combined for all families analyzed (tm-ts = 0. 077) was also positive and significant, suggesting that 7% of crossings that occur in this population are between related individuals. The correlation of selfing negative(rs=-0.999), indicates absence of selfing and the correlation of paternity(rp=0.107) not significantly different from zero (SD = 0.135) shows that this subpopulation no full siblings. Every subpopulation resulting outcrossing and individuals are evaluated relatives at least to the 15 level of half-brothers. The paternity analysis assigned pollen donors to 75.2% (64) seedlings, and 24 (37.5%) assignments at a confidence level of 85%, 30 (46.8%) assignments at 95% probability assignments and 10(15.6%) at a confidence level of 99%.This low assignment can be explained by sampling: due to the size of the total area of the population and distribution in aggregate, many individuals may not have been sampled. Moreover, the loci battery used in this analysis could not demonstrated the optimal values for the combined exclusion. Although the maximum distance of pollination has been of 292m and covers the entire area evaluated, most events of pollination (77%) occurred at distances less than 200m. The flowering in mass, the distribution in aggregate and the floral structure are the main responsible for predominance of events of cross-pollination at short distances.