FM - Artigos publicados em periódicos

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Estudo imunobiologico de estoques de 'Trypanosoma cruzi' isolados de pacientes na fase aguda da doenca de Chagas
(1983-12) Kipnis, Thereza Liberman; Minóprio, Paola Marcella; Ostermayer, Alejandro Luquetti; Rassi, Anis; Silva, Wilmar Dias da
Radiolabed total parasite DNA probe specifically detects Trypanosoma cruzi in mammalian blood
(1988-03) Ashall, Frank; Yip-Chuck, Deborah; Ostermayer, Alejandro Luquetti; Miles, Michael
A DNA-DNA hybridization procedure is described in which radiolabeled total parasite DNA is used to detect Trypanosoma cruzi spotted directly onto nylon membrane. Under conditions that favor hybridization of repetitive DNA sequences, the radiolabeled total DNA was able to detect as few as five parasites spotted onto nylon membrane. Trypanosomes were detectable in blood from mice with T. cruzi parasitemias spotted directly onto membranes. Ali 10 strains of T. cruzi examined, which came from different areas of the United States and South America, were readily detected with a total DNA probe from any 1 strain of the parasite. No signals were detected with up to 20,000 Trypanosoma rangeli, T. brucei, Crithidia fasciculata, or Leishmania parasites or with normal mouse blood or mammalian DNA. The hybridization method is sensitive, specific, rapid, and inexpensive and is potentially applicable to the detection of other parasites.
Mass screening for Trypanosoma cruzi infections using the immunofluorescence, ELISA and haemagglutination tests on serum samples and on blood eluates from filter-paper
(1990) Zicker, Fabio; Smith, Peter George; Ostermayer, Alejandro Luquetti; Oliveira, O. S.
Methods used to diagnose Trypanosoma cruzi infection differ in their ability to discriminate between sera from infected and uninfected individuals. We compared the results of an immunofluorescence (IF) test, a haemagglutination (HA) test, and an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of T. cruzi infections in a large population-based survey in central Brazil using blood eluates from filter-paper and venous blood samples. The sensitivities of the tests on eluates, compared with results on serum samples, were low: ELISA (78. 1%), IF (69.2%) and HA (64.6%). The level of agreement between the tests on eluates was very poor, with the best co-positivity for IF and ELISA. Both the positive and negative predictive values of the three tests on eluates were similar (around 96%) to those for sera. Higher co-positivity values were obtained for the three tests on sera. The implications of these results are discussed in relation to blood screening, routine medical practice, sero-epidemiological surveys, and the follow-up of patients admitted to therapeutic trials.
Surveillance of Trypanosoma cruzi transmission by serological screening schoolchildren
(1992) Andrade, Ana Lúcia Sampaio Sgambatti de; Zicker, Fabio; Ostermayer, Alejandro Luquetti; Oliveira, R. M.; Silva, Simonne Almeida e; Souza, José Maria Pacheco de; Martelli, Celina Maria Turchi
The seroprevalence of Trypanosoma cruzi infection among children is a sensitive indicator for assessing the effectiveness of programmes for control of Chagas disease. In this study we report the result of a cross-sectional serological survey carried out among schoolchildren living in a poor rural area in central Brazil. Eluates of blood collected on filter-paper were tested for anti-T. cruzi antibodies using immunofluorescence, haemagglutination, and enzyme-linked immunosorbent assays. The overall seroprevalence of T. cruzi infection was 7.9%, which compared with the findings of the national survey carried out in 1975-80 indicates that a twofold-to-threefold reduction in prevalence has occurred over the last 10 years. This is consistent with a reduction of transmission in the area, probably related to vector control efforts. Based on our results, the incidence of new cases was estimated to be 44 per annum in the study region. In rural areas with a scattered population, surveillance of T. cruzi transmission by serological screening of children at school entry is more practical and economical than entomological evaluation for assessing both the risk of transmission in the community and the efficacy of vector control measures. A sample size of around 1000 schoolchildren is sufficient to detect prevalences as low as 2%, and such an approach would be practical and applicable to most areas where Chagas disease is endemic.
Viabilidade da utilização da pasta Guedes-Pinto na pulpotomia de dentes decíduos: revista da literatura
(1992) Costa, Luciane Ribeiro de Rezende Sucasas da; Lopes-Marotti, Noely Regina
Restaurações complexas de amalgama retidas por amalgapins
(1992) Taveira, William Sebastião; Rocha, Luisa Isabel Taveira; Costa, Luciane Ribeiro de Rezende Sucasas da; Esber, Mahassen
Serologic screening for Trypanosoma cruzi among blood donors in central Brazil
(1992) Andrade, Ana Lúcia Sampaio Sgambatti de; Martelli, Celina Maria Turchi; Ostermayer, Alejandro Luquetti; Oliveira, O. S. de; Silva, Simonne Almeida e; Zicker, Fabio
The study reported here compares results obtained by blood banks screening sera for chagasic (Txypanosoma cruzi) infecfion with results obtained by the Chagas’ Disease Reference Laboratory of the Federal University of Goia’s in GoiJnia, Brazil. If also evaluates results obtained using the ELISA technique to screen the study sera. The survey used dafa from six of eight blood banks serving the city of Goirinia, an urban region of Central Brazil where Chagas’ disease is highly endemic. The survey population consisted of 1,513 voluntary firsttime blood donors whose donations occurred between Ocfober 1988 and April 1989. This group included 50% of all the first-time blood donors in that period. The six participating blood banks, which accounted for about 90% of all blood donations in Goilinia during the study period, routinely used indirecf hemagglutination (IHA) and complemenf fixafion (CF) tests to screen sera for antibodies to T. cruzi. Comparison of the resulfs provided by fhe blood banksw ith the referencela boratory’s resulfs indicated a relative sensifivity of 77%, which ranged from 50% to 100% depending on the blood bank studied. The comparison, which found 12 false negative resulfs, indicated that transfusions of infected blood might have occurred despite the serologic screening performed by the blood banks. Relative fo the standard of posifivity established for the study, the enzyme-linked immunosorbenf assay (ELISA) technique was found to have a sensifivify of 96.3%. Considering as pbsitive only those seray ielding positive IHA and indirect immunofluorescence(I IF) test results, the ELISA techniquey ielded 2 false negative and 41 false posifive responsesA. mong the 41 were 34 sera found positive by the ELISA technique alone. These results point to a need for assessing the costs and benefits of introducing the ELISA technique in parallel with blood banks’ serologic fesfing and underscore the desirability of establishing a coherent overall system for moniforing the screening of donated blood for T. cruzi infecfion.
Restaurações complexas de amalgama retidas por amalgapins. Parte I: revisão da literatura
(1992) Rocha, Luisa Isabel Taveira; Taveira, Willian Sebastião; Costa, Luciane Ribeiro de Rezende Sucasas da; Gonçalves, Alberto Magno; Esber, Mahassen Hanna
Aneurismas dissecantes intracranianos da circulação posterior: relato de três casos e revisão da literatura
(1992) Diniz, Denise Sisterolli; Portela, L. A. P.; Melo-Souza, Sebastiao Eurico de
Dissecting aneurysms of cerebral arteries are unusual causes of stroke. The carotid system is the commonest site of this pathology, the vertebral arteries are less involved and dissection of the basilar artery is rtare. The authors report three cases of arterial dis- section of the vertebrobasilar system, two of the vertebral arteries and one of the basilar artery. An extensive review of the literature is presented. The clinical picture of dissection of vertebrobasilar system was inespecific but pain was a prominente symptom, though had not occurred in the site of the arteries involved. The pain was suggestive of subarachnoid hemorrhage. Associated or risk factors were mild trauma, migraine and high blood pressure. The angiographic findings were suggestive, however just the «double lumen» has been con- sidered pathognomonic. The prognosis is variable. It was benign in case 3, left sequela in case 2, and case 1 rebleed fatally.
Análise comparativa "in vitro" do selamento marginal, da obturação de canais radiculares, quando da utilização de cones de guta-percha de dupla procedência
(1993) Pesce, Hildeberto Francisco; Medeiros, João Marcelo Ferreira de; Estrela, Carlos
Trypanosoma cruzi and experimental Chagas´disease: characterization stock isolated from a patient with associated digestive and cardiac form
(1993-03) Oliveira, Enio Chaves de; Stefani, Mariane Martins de Araújo; Ostermayer, Alejandro Luquetti; Vêncio, Eneida Franco; Moreira, Marise Amaral Rebouças; Souza, C.; Rezende, Joffre Marcondes
Importância do método de obtenção das dejeções dos triatomíneos na avaliação da suscetibilidade triatomínica para Trypanosoma cruzi
(1993-03) Silva, Ionizete Garcia da; Ostermayer, Alejandro Luquetti; Silva, Heloisa Helena Garcia da
Apical leakage using various sealers and root canal filling techniques
(1994) Estrela, Carlos; Pesce, Hildeberto Francisco; Sydney, Gilson Blitzkow; Figueiredo, Jose Antonio Poli de
Radicular grooves in maxillary lateral incisor: case report
(1995) Estrela, Carlos; Lopes, Hélio Pereira; Pecora, Jesus Djalma
The authors present a case report of a radicular groove in a maxillary lateral incisor in a 58-year old male patient. Endodontic and periodontal treatment was not carried out and therefore, avulsion of the tooth was indicated. The radicular grooves were very deep with pulp chamber communication.
Avaliação da dor e de testes de vitalidade para o diagnóstico da inflamação pulpar
(1995) Estrela, Carlos; Lopes, Hélio Pereira; Resende, Edison Vivas de; Alencar, Ana Helena Gonçalves de
Mechanism of the action of calcium and hydroxy ions of calcium hydroxide on tissue and bacteria
(1995) Estrela, Carlos; Sydney, Gilson Blitzkow; Bammann, Lili Luescke; Felippe Junior, Oswaldo
The biological and bacteriological action of calcium hydroxide confer to it its current success as an intracanal dressing. For this reason the mechanism of action of calcium and hydroxyl ions on tissue and bacteria deserves further study. The objective of the present paper is to analyze and discuss the mechanism of action of calcium and hydroxyl ions on anaerobic bacteria, starting from the isolated study of the influence of pH on these bacteria, as well as the mechanism of action of calcium hydroxide on tissue.
Diferentes terapias no tratamento da obesidade em pacientes hipertensos
(1996) Monego, Estelamaris Tronco; Peixoto, Maria do Rosário Gondim; Jardim, Paulo Cesar Brandao Veiga; Sousa, Ana Luiza Lima; Braga, Vera Lúcia N.; Moura, Milton Cesar Ferlin
Purpose - To evaluate the efficacy and tolerability of different therapies associated with diet in the treatment of hypertensive obese patients. Methods - In a clinical study we randomly evaluated 39 hypertensive obese patients (body mass index (BMI) >30kg/m 2 ). After 45 days of diet the patients were again randomly distributed in 3 groups and received in double blind way: group 1 - hypocaloric diet + placebo; group 2 - hypocaloric diet + dexfenfluramine; group 3 - hypocaloric diet + spiruline+fucus+gelatine. We followed their progress during 12 weeks under medication and further 24 weeks without. We evaluated the groups comparing: weight, BMI, blood pressure and side-effects. Results - Twenty seven patients completed the obser- vation. In those patients from groups 1 and 3 no changes in any of parameters were observed. In group 2 we observed a clear loss of weight (-3.8Kg) and a fall in BMI. Blood Pressure changes were only observed in group 2 (-9.6%). The only patients to maintain weight loss after the termination of use of medicines were those from group 2. We did not observe any side-effects. Conclusion - In hypertensive obese patients, when isolated diet is not enough to control weight loss, dexfenflu- ramine could be useful in association with a controlled diet. The drug assists in weight loss, does not promote side- effects and does not interfere in the treatment of blood pres- sure.
Influência da substância química, do cimento obturador e do número de sessões na incidência de pericementite traumática
(1996) Estrela, Carlos; Siqueira, Regis Murilo Gomes; Resende, Edison Vivas de; Silva, Simone Almeida da; Silva, Flavio Augusto Cardoso da
Influência do acesso endodôntico na localização dos canais radicluares nos molares superiores
(1996) Savioli, Ricardo Novak; Silva, Ricardo Gariba; Cruz Filho, Antonio Miranda da; Estrela, Carlos; Pécora, Jesus Djalma
Retratamento endodôntico - técnicas e meios auxiliares
(1996) Tanomaru Filho, Mario; Alencar, Ana Helena Gonçalves de; Araújo, Claudia Guimarães

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